A939572

Manufactured by Merck Group

A939572 is a type of laboratory equipment manufactured by Merck Group. It is designed to perform specific functions in a laboratory setting. The core function of this product is to [insert brief, factual description of the equipment's primary purpose or capability, without interpretation or extrapolation].

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Lab products found in correlation

Oleate, by Merck Group (2 mentions) Myriocin, by Merck Group (1 mentions) Glucose uptake fluorometric assay kit, by Merck Group (1 mentions) Ceranib 2, by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Ab81484, by Abcam (1 mentions) Dual luciferase reporter dlr assay system, by Promega (1 mentions) Protein lysis buffer, by Promega (1 mentions) Glomax 20 20 luminometer, by Promega (1 mentions)

2 protocols using a939572

Biochemical Analysis of Insulin Signaling

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All reagent-grade chemicals including insulin, TOFA, FB1, myriocin, ceranib-2, A939572, oleate, and stearate were obtained from Sigma-Aldrich. C2-cer was obtained from Merck Chemicals. C-75 was from Tocris Bioscience. Antibodies against ⁴⁷³Ser-Akt, ³⁰⁸Thr-Akt, native PKB/Akt, GLUT4, and α1 NA-K-ATPase were from Cell Signaling Technology, and antibody against ceramide (MID 15B4 clone) was from Enzo Life Sciences. Horseradish peroxidase anti-rabbit and horseradish peroxidase anti-mouse were from Jackson ImmunoResearch Laboratories, and the enhancer chemiluminescent Supersignal was from Thermo Fisher Scientific. Ceramide secondary antibody (Alexa fluor 488 goat anti-mouse) was from Thermo Fisher Scientific. Glucose uptake fluorometric assay kit was from Sigma-Aldrich.

Bandet C.L., Tan-Chen S., Ali-Berrada S., Campana M., Poirier M., Blachnio-Zabielska A., Pais-de-Barros J.P., Rouch C., Ferré P., Foufelle F., Le Stunff H, & Hajduch E. (2023). Ceramide analog C2-cer induces a loss in insulin sensitivity in muscle cells through the salvage/recycling pathway. The Journal of Biological Chemistry, 299(6), 104815.

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Inhibition of Wnt Signaling Pathway

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Transfection cocktail was made using TransIT-Keratinocyte reagents according to the manufacturer's instruction (Mirus, WI, USA). After 16 hours, media was refreshed with inhibitors, A939572: 1 µM, TMP-153 sc-200649 : 0.5 µM, Oleate: 12.5 µM (Sigma). After 16 hours of inhibitor pretreatment, cells were refreshed with media containing inhibitors with or without Wnt3a (50 ng/mL) (ab81484, Abcam, MA, USA). After 24 hours, we harvested cells with 50 µL protein lysis buffer (Promega, WI, USA), freeze-thawed 3 cycles, and determined TOP Flash activity by dual-luciferase™ reporter (DLR™) assay systems (Promega) using spectrophotometer (Glomax 20/20 Luminometer, Promega).

Jain P., Nattakom M., Holowka D., Wang D.H., Brenna J.T., Ku A.T., Nguyen H., Ibrahim S, & Tumbar T. (2018). Runx1 role in epithelial and cancer cell proliferation implicates lipid metabolism and Scd1 and Soat1 activity. Stem cells (Dayton, Ohio), 36(10), 1603-1616.

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