The CIA model was established according to a previously described protocol (19 (link)). Briefly, 8 mg type II collagen (CII) (Chondrex, Redmond, WA, USA) was dissolved in 0.1 mol/l acetic acid and vortexed at 4°C; the concentration of CII reached 2 mg/ml. A total of 100 µg CII was emulsified thoroughly with the same volume of complete Freund's adjuvant (Chondrex) in an ice bath; the final concentration of CII reached 1 mg/ml. A total of 50 rats were injected subcutaneously at the tail base with 200 µl CII emulsion for the first immunization. After 7 days, 100 µg CII was dissolved and emulsified at the same concentration using incomplete Freund's adjuvant (Chondrex), and 100 µl emulsion was subcutaneously administered into the tail as a booster injection. Clinical arthritis was measured and the Arthritis Index (AI) was analyzed, as presented in Table I . The AI for each rat was expressed as the sum of the scores for all four limbs; therefore, the maximum AI was 16.
Type 2 collagen cii
Manufactured by Chondrex
Sourced in United States
Type II collagen (CII) is a key structural component of articular cartilage. It is a fibrillar collagen that provides tensile strength and contributes to the unique biomechanical properties of cartilage.
Automatically generated - may contain errors
Lab products found in correlation
Incomplete freund s adjuvant, by Chondrex (1 mentions)
Complete freund s adjuvant, by Chondrex (1 mentions)
Ionomycin, by Thermo Fisher Scientific (1 mentions)
Icosl, by BioLegend (1 mentions)
Phorbol 12 myristate 13 acetate, by Thermo Fisher Scientific (1 mentions)
Cd62l, by Thermo Fisher Scientific (1 mentions)
Interleukin 4 (il 4), by BioLegend (1 mentions)
Il 22, by BioLegend (1 mentions)
Hyaluronidase, by Merck Group (1 mentions)
Ifn γ, by BioLegend (1 mentions)
3 protocols using type 2 collagen cii
1
Collagen-Induced Arthritis (CIA) Model
2
Characterization of Immune Cell Populations
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Type II collagen (CII) was purchased from Chondrex (Redmond, WA, USA). Hyaluronidase was obtained from Sigma-Aldrich (St. Louis, MO, USA). Phorbol 12-myristate 13-acetate (PMA) and ionomycin were all products from eBioscience (San Diego, CA, USA). Fluorochrome-conjugated anti-human monoclonal antibodies (mAbs) including CD4 (clone: RPA-T4), CD14 (clone:M5E2), CD19 (clone: HIB19), ICOS (clone:C398.4A), ICOSL (clone:2D3) were from Biolegend (San Diego, CA, USA). Fluorochrome-conjugated anti-mouse mAbs including CD3 (clone:145-2C11), CD19 (clone:6D5), NK1.1 (clone:PK136), ICOS (clone:15F9), ICOSL (clone:HK5.3), CD44 (clone:IM7), B220 (clone:RA3-6B2), CD138 (clone:281-2), GL7 (clone:GL7), CD25 (clone:3C7), Foxp3 (clone:150D), CXCR5 (clone:L138D7), IFN-γ (clone:XMG1.2), TNF-α (clone:MP6-XT22), IL-4 (clone:11B11), IL-9 (clone:RM94A), IL-17 (clone:TC11-18H10.1) and IL-22 (clone:poly5164) were from Biolegend. Fluorochrome-conjugated anti-mouse mAbs CD4 (clone: GK1.5), CD38 (clone:90), CD95 (clone:15A7), CD62L (clone: MEL-14), IL-6 (clone:MP5-20F3), IL-21 (clone: FFA21) were from eBioscience. Anti-mouse CD11b mAb was from Mitenyi Biotec (Bergisch Gladbach, Germany). The anti-mouse ICOSL blocking antibody (clone HK5.3) was from Biolegend.
3
Mesenchymal Stem Cells for Collagen-Induced Arthritis
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A total of 24 DBA/1 mice were randomly assigned to four experimental groups: collagen-induced arthritis (CIA) mice (CIA), CIA mice infused with UCs (UC), CIA mice infused with BMSCs (BMSC), and CIA mice infused with SHED (SHED). CIA model was induced as described previously (Brand, Latham & Rosloniec, 2007 (link)). Briefly, DBA/1 mice received an injection into the base of the tail with 100 μg of type II collagen (CII) (Chondrex, Redmond, WA, USA) emulsified in Freund’s complete adjuvant. A total of 15 days later, they received booster injections of 100 μg of type II collagen in Freund’s incomplete adjuvant. On day 30, 1 × 106 MSCs dissolved in 200 μl PBS per mouse were administered via tail vein injection to three groups (UC, BMSC, and SHED) and an equal volume of PBS was administered to the control group (CIA).
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