Bsm 52396r

The sections from cardiac tissues were examined using the ultravision quanto detection system procedure (TL-125-QHD, Thermo Fisher Scientific). The cardiac tissue sections were incubated with anti-THBS2 (1:200, A8561, ABclonal), α-SMA (1:400, bsm-52396R, Bioss Antibodies Inc), β-catenin (1:400, GTX632676, Gene Tex), fibronectin (1:200, ab2413, Abcam) and vimentin (1:400, SC-7557, Santa Cruz Biotechnology) antibodies at 4 °C overnight. Then, the sections were treated with a biotinylated anti-mouse secondary antibody for 1 h. The slides were counterstained with Mayer’s hematoxylin for 2 min and then mounted. The images were acquired using Nikon’s Eclipse E600 research microscope (Nikon, Tokyo, Japan).

Citronellal (CT, Beijing Yuke Biotechnology Co., Ltd) was purchased form Beijing, China. streptozotocin (STZ), metformin (DMBG), acetylcholine (Ach) and sodium nitroprusside (SNP) were purchased from Signal-Aldrich, St. Louis, MO, USA. The kits for MDA (A003-1-2), NO (C009-2-1), ROS (E004-1-1), and SOD (A001-3-2) were obtained from Nanjing Jiancheng, Nanjing, China. Dihydroethidium (DHE, S0063, Beyotime), ROS assay kit (ROS, S0033S, Beyotime), and JC-1-mitochondrial membrane potential assay kit (C2003S, Beyotime) were obtained from Beyotime, shanghai, China. The anti-NOX2 antibody (ab129068, Abcam), anti-NOX4 antibody (ab154244, Abcam), anti-DRP1 antibody (ab56788, Abcam), anti-TRPM2 antibody (ab11168, Abcam) and anti-VDAC (ab154856, Abcam) were obtained from Abcam, Cambridge, MA, USA. The anti-NHE1 antibody (bs-0505R, Bioss) and anti-α-SMA antibody (bsm-52396R, Bioss) were obtained from Bioss, Beijing, China. The anti-GAPDH antibody (AB0036, Abways) was acquired from Shanghai, China.