Recombinant human TGF-β1 was obtained from R&D Systems (Minneapolis, MN). The following antibodies were used for the study: mouse anti-human TGF-β1 monoclonal antibody (mAb) (1 : 50, Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-human Smad2/3 polyclonal Ab (pAb) (1 : 100, R&D Systems, Minneapolis, MN, USA), goat anti-human p-SMAD2/3 (Ser 423/425) pAb (1 : 100, Santa Cruz Biotechnology), mouse anti-human SMAD4 mAb (1 : 50, Santa Cruz Biotechnology), rabbit anti-human Snail mAb (1 : 50, Cell Signaling Technology, Danvers, MA), mouse anti-human β-actin mAb clone AC-15 (1 : 100, Sigma-Aldrich, St. Louis, MO), mouse anti-human E-cadherin mAb (1 : 100, Dako, Santa Clara, CA, USA), mouse anti-human vimentin mAb (1 : 100, Thermo Fisher Scientific, Waltham, MA, USA), and rabbit anti-human collagen type I pAb (1 : 500, Thermo Fisher Scientific, Waltham, MA, USA).
Mouse anti human e cadherin mab
Manufactured by Agilent Technologies
Sourced in Macao
The mouse anti-human E-cadherin monoclonal antibody (mAb) is a laboratory reagent used for the detection and analysis of the E-cadherin protein in human cell and tissue samples. E-cadherin is a cell-cell adhesion molecule that plays a crucial role in the maintenance of epithelial cell-cell contacts and tissue integrity.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti human tgf β1 monoclonal antibody mab, by Santa Cruz Biotechnology (3 mentions)
Goat anti human smad2 3 polyclonal ab pab, by R&D Systems (3 mentions)
Goat anti human p smad2 3 ser 423 425 pab, by Santa Cruz Biotechnology (3 mentions)
Mouse anti human β actin mab clone ac 15, by Merck Group (3 mentions)
Mouse anti human vimentin mab, by Thermo Fisher Scientific (3 mentions)
Recombinant human tgf β1, by R&D Systems (2 mentions)
Erk inhibitor u0126, by Merck Group (1 mentions)
Sb431542, by R&D Systems (1 mentions)
3 protocols using mouse anti human e cadherin mab
1
TGF-β1 Signaling Pathway Evaluation
2
TGF-β1 Signaling Pathway Analysis
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Recombinant human TGF-β1 was purchased from R&D Systems (Minneapolis, MN, USA). Mouse anti-human TGF-β1 monoclonal antibody (mAb) (1:50, Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-human SMAD2/3 polyclonal Ab (pAb) (1:100, R&D Systems, Minneapolis, MN, USA), goat anti-humanp-SMAD2/3 (Ser 423/425) pAb (1:100, Santa Cruz Biotechnology), mouse anti-human β-actin mAb clone AC-15 (1:100, Sigma-Aldrich, St. Louis, MO, USA), mouse anti-human E-cadherin mAb (1:100, Dako, Santa Clara, CA, USA), mouse anti-human vimentin mAb (1:100, Thermo Fisher Scientific, Waltham, MA, USA), human Follistatin-like 1/FSTL1 polyclonal Antibody (pAb) (1 μg/mL, R&D Systems) were used in the experimental procedure.
3
Interleukin-17 Signaling Pathway Modulation
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Recombinant human Interleukin-17 (IL-17) (50 ng/ml) was purchased from Sigma-Aldrich (St. Louis, MO). The following antibodies were used for the study: mouse antihuman TGF-β1 monoclonal antibody (mAb) (1:50, Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat antihuman Smad2/3 polyclonal Ab (pAb) (1:100, R&D Systems, Minneapolis, MN, USA); goat anti-human p-Smad2/3 (Ser 423/425) pAb (1:100, Santa Cruz Biotechnology), mouse anti-human Erk1/Erk2 mAb or mouse anti-human phospho-Erk1/Erk2 mAb (both from R&D systems); mouse anti-human β-actin mAb clone AC-15 (1:100, Sigma-Aldrich, St. Louis, MO); mouse anti-human E-cadherin mAb (1:100, Dako, Santa Clara, CA, USA), mouse anti-humanvimentin mAb (1:100, Thermo Fisher Scientific, Waltham, MA, USA); TGF-β receptor type I kinase inhibitor SB-431542 (10 mM in DMSO) (R&D systems, Minneapolis, MN, USA); Erk inhibitor U0126 (Sigma-Aldrich) at 10 μM.
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