Log In Sign up
The largest database of trusted experimental protocols

Succinate acid disodium

Manufactured by Merck Group
Visit Supplier

Succinate acid disodium is a chemical compound with the formula C4H4Na2O4. It is a salt of succinic acid and is commonly used as a pH regulator, buffer, and intermediate in various chemical reactions and processes.

Automatically generated - may contain errors

Lab products found in correlation

N6876, by Merck Group (2 mentions) H 1000, by Vector Laboratories (2 mentions) Evos fl light microscope, by Thermo Fisher Scientific (2 mentions) Nitroblue tetrazolium, by Merck Group (1 mentions)

3 protocols using succinate acid disodium

1

Visualizing SDH Content in Muscle Fibers

Check if the same lab product or an alternative is used in the 5 most similar protocols
SDH content within muscle fibers was visualized following 1-h incubation at 37 °C in the following solution: nitro blue tetrazolium (Sigma) and succinate acid disodium (Sigma) and dissolved in 0.2 M of phosphate-buffered saline (PBS). The reaction was performed in a light protected coplin jar, and sections were then sequentially rinsed with 30 and 60 % acetone before being mounted with aqueous mounting media. Images were captured at ×20 magnification, were manually assessed for SDH staining intensity, and were categorized as positive, weakly positive, or negative fibers (++, +, −). The assessor was blinded to the treatment of the animals.
Jackson J.R., Kirby T.J., Fry C.S., Cooper R.L., McCarthy J.J., Peterson C.A, & Dupont-Versteegden E.E. (2015). Reduced voluntary running performance is associated with impaired coordination as a result of muscle satellite cell depletion in adult mice. Skeletal Muscle, 5, 41.
+ Open protocol
+ Expand
2

Muscle Fiber Oxidative Capacity Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Muscle sections (see Muscle fiber-typing) were incubated in freshly-made SDH staining solution [0.367 mM nitrotetrazolium blue (N6876, Sigma) and 60 mM succinate acid disodium (224731, Sigma) in 1x PBS pH 7.4 (10010, Gibco)]. Sections were incubated at 37°C for 1 hour. Sections were rinsed in 30%, 60%, and 30% Acetone for 1 minute, followed by a three rinses with distilled H2O. Sections were then mounted (H-1000, Vector Laboratories) and imaged at 10x using Evos FL light microscope (Life Technologies Corp., CA, USA).
Verkerke A.R., Ferrara P.J., Lin C.T., Johnson J.M., Ryan T.E., Maschek J.A., Eshima H., Paran C.W., Laing B.T., Siripoksup P., Tippetts T.S., Wentzler E.J., Huang H., Spangenburg E.E., Brault J.J., Villanueva C.J., Summers S.A., Holland W.L., Cox J.E., Vance D.E., Neufer P.D, & Funai K. (2019). Phospholipid methylation regulates muscle metabolic rate through Ca2+ transport efficiency. Nature metabolism, 1(9), 876-885.
+ Open protocol
+ Expand
3

Muscle Fiber Oxidative Capacity Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Muscle sections (see Muscle fiber-typing) were incubated in freshly-made SDH staining solution [0.367 mM nitrotetrazolium blue (N6876, Sigma) and 60 mM succinate acid disodium (224731, Sigma) in 1x PBS pH 7.4 (10010, Gibco)]. Sections were incubated at 37°C for 1 hour. Sections were rinsed in 30%, 60%, and 30% Acetone for 1 minute, followed by a three rinses with distilled H2O. Sections were then mounted (H-1000, Vector Laboratories) and imaged at 10x using Evos FL light microscope (Life Technologies Corp., CA, USA).
Verkerke A.R., Ferrara P.J., Lin C.T., Johnson J.M., Ryan T.E., Maschek J.A., Eshima H., Paran C.W., Laing B.T., Siripoksup P., Tippetts T.S., Wentzler E.J., Huang H., Spangenburg E.E., Brault J.J., Villanueva C.J., Summers S.A., Holland W.L., Cox J.E., Vance D.E., Neufer P.D, & Funai K. (2019). Phospholipid methylation regulates muscle metabolic rate through Ca2+ transport efficiency. Nature metabolism, 1(9), 876-885.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!