Tritiated glycine

Manufactured by PerkinElmer

Tritiated glycine is a radiolabeled amino acid used as a research tool in biological and biochemical studies. It serves as a tracer to investigate metabolic pathways, protein synthesis, and other cellular processes. The tritium label allows for the detection and quantification of the compound within experimental systems.

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Lab products found in correlation

Solvable, by PerkinElmer (1 mentions) Ultima gold, by PerkinElmer (1 mentions) Ethyl chloroformate ecf, by Merck Group (1 mentions) 2 4 chlorophenyl ethylamine, by Merck Group (1 mentions) Thymidine 5 monophosphate disodium salt, by Merck Group (1 mentions) 2 3h glycine, by PerkinElmer (1 mentions)

2 protocols using tritiated glycine

Radiolabeled PolySTAT Biodistribution in Rats

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Radiolabeled PolySTAT was synthesized by mixing in tritiated glycine (Perkin Elmer) to fibrin-binding peptides for conjugation to p(HEMA-co-NHSMA) copolymers (1 glycine for every 200 PolySTAT). Rats were anesthetized using isoflurane and ketamine-xylazine cocktail injection in the hindlimb. 15 mg/kg radiolabeled PolySTAT was administered via tail-vein injection. At the desired timepoints (5 min, 10 min, 20 min, 1 hr, and 1 day), the thoracic cavity was opened and blood was collected from the right atrium of the heart using a syringe. Rats were then perfused, and organs were rinsed, weighed, and homogenized. Tissue homogenates were solubilized using Solvable (Perkin Elmer) for 2 hours at 50°C and decolorized for 4–6 hours by addition of 30% (v/v) hydrogen peroxide and 100 mM EDTA solution to prevent bubbling. 20 µL 10 N HCl and 10 mL Ultima Gold (Perkin Elmer) was then added and samples were shaken vigorously and allowed to sit overnight before reading in a scintillation counter.

Chan L.W., Wang X., Wei H., Pozzo L.D., White N.J, & Pun S.H. (2015). A Synthetic Fibrin-Crosslinking Polymer for Modulating Clot Properties and Inducing Hemostasis. Science translational medicine, 7(277), 277ra29.

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Enzymatic Assay for Folate Metabolism

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Chemicals and reagents 2-(4-chlorophenyl)ethylamine, ethyl chloroformate (ECF), diethyl ether, ethyl acetate, dichloromethane and thymidine 5′-monophosphate disodium salt were obtained from Sigma-Aldrich. Tetrahydrofolate and (6S)-5-formyl tetrahydrofolate were kindly provided by Merck & Cie (Schaffhausen, Switzerland). Tritiated glycine used in the radioisotopic assay ([2−3H]glycine) was purchased from Perkin Elmer. (6R,S)-5-Formyl-5,6,7,8-tetrahydropteroylpenta-γ-L-glutamic acid lithium salt (5-CHO-THF-Glu₅) was purchased from Schircks Laboratories, Bauma, Switzerland. All other reagents used in the enzymatic assays were obtained from Sigma-Aldrich.

Guiducci G., Paone A., Tramonti A., Giardina G., Rinaldo S., Bouzidi A., Magnifico M.C., Marani M., Menendez J.A., Fatica A., Macone A., Armaos A., Tartaglia G.G., Contestabile R., Paiardini A, & Cutruzzolà F. (2019). The moonlighting RNA-binding activity of cytosolic serine hydroxymethyltransferase contributes to control compartmentalization of serine metabolism. Nucleic Acids Research, 47(8), 4240-4254.

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