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Flag tag (flag)

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The Flag is a laboratory equipment used for filtering liquids. It consists of a perforated surface that allows the passage of the liquid while retaining solid particles. The specific design and materials used in the Flag can vary depending on the intended application and requirements of the user.

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Lab products found in correlation

C myc, by GeneTex (2 mentions) C myc, by Abcam (2 mentions) C myc, by Cell Signaling Technology (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Cycloheximide (chx), by Yeasen (2 mentions) Usp16, by Merck Group (2 mentions) Mg132, by Yeasen (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Gfp 50430 2 ap, by Proteintech (1 mentions) Ikzf1, by Cell Signaling Technology (1 mentions) Anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Lamin b, by Cell Signaling Technology (1 mentions) Uhrf1, by Cell Signaling Technology (1 mentions) Plvx vector, by Takara Bio (1 mentions) Puromycin, by Merck Group (1 mentions) C myc, by Takara Bio (1 mentions) Pei 25k, by Polysciences (1 mentions)

Spelling variants of this product

Anti-FLAG-tag (3 citations)

3 protocols using flag tag (flag)

1

Protein Interaction Analysis Techniques

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MG132(52801ES08) and CHX (40325ES03) were purchased from Yeason (Shanghai, China). The following antibodies were used for western blotting: USP16 (A5861; Abclonal), c-Myc (GTX103436; GeneTex), β-actin (sc-47,778; Santa Cruz Biotechnology). Antibodies used for immunohistochemistry: USP16 (HPA021140; Sigma-Aldrich), Ki67 (sc-15,402; Santa Cruz), c-Myc (#ab32072, Abcam). Antibodies used for immunoprecipitation and immunofluorescence: Flag (#30503ES60, Yeason), USP16 (HPA021140; Sigma-Aldrich), c-Myc (13987S, Cell Signaling Technology).
Ge J., Yu W., Li J., Ma H., Wang P., Zhou Y., Wang Y., Zhang J, & Shi G. (2021). USP16 regulates castration-resistant prostate cancer cell proliferation by deubiquitinating and stablizing c-Myc. Journal of Experimental & Clinical Cancer Research : CR, 40, 59.
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2

Antibody Source Catalog for Diverse Protein Studies

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The sources of antibodies against the following proteins were as follows: IKZF1(14859, Cell Signaling Technology); USP7 (A700-072, Bethyl Laboratories); Lamin B (12586, Cell Signaling Technology); GFP (50430-2-AP, Proteintech); FLAG (30503ES20, Yeasen); UHRF1 (12387, Cell Signaling Technology); P5091 (882257-11-6, ChemShuttle); His (66005-1-Ig, Proteintech); Myc (2276, Cell Signaling Technology); Ub (10201-2-AP, Proteintech); VP1 (633419-42-0, Sigma); CPT (CSN16581, CSNpharm); Olaparib (CSN12345, CSNpharm); 4-OHT (CSN22105, CSNpharm); CtIP (GTX70264, GeneTex); RPA2 (A2189, abclonal); Phospho-RPA2-S4/S8 Rabbit pAb (AP1102, abclonal); RAD51 (ab133534, Abcam); lenalidomide (CSN11280, CSNpharm); HA (AE008, abclonal); Rabbit mAb (9532, Cell Signaling Technology); β -Actin-HRP (conjugated) (AB2001, Abways); Anti-rabbit IgG, HRP-linked Antibody (7074, Cell Signaling Technology); γH2AX (80312, Cell Signaling Technology); Anti-Alexa Fluor 555 (P0190, Beyotime Biotechnology).
Liu M., Zhang Y., Wu Y., Jin J., Cao Y., Fang Z., Geng L., Yang L., Yu M., Bu Z., Ji Y., Shan H., Zou Z., Liu L., Wang Y., Zhang Y., Tong Y., Xu H., Lei H., Liu W., Gao F, & Wu Y. (2022). IKZF1 selectively enhances homologous recombination repair by interacting with CtIP and USP7 in multiple myeloma. International Journal of Biological Sciences, 18(6), 2515-2526.
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3

Identifying USP16 and c-Myc Interactions

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Short hairpin RNA (shRNA) sequences are listed in Additional le 1: Table S1. These sequences were cloned into the pLKO.1 vector, while Flag-tagged USP16 and c-Myc were cloned into the pLVX vector (Clontech 632187). Plasmids were transfected into HEK293FT cells using PEI 25K (23966-1; Polysciences, Warrington, PA, USA) according to the manufacturer's instructions. Stable transformants of PC3 and DU145 cells were isolated in standard medium supplemented with puromycin (5 μg/mL) (Sigma-Aldrich, St. Louis, MO, USA) for 7 days.
Reagents and primary antibodies MG132(52801ES08) and CHX (40325ES03) were purchased from Yeason (Shanghai, China). The following antibodies were used for western blotting: USP16 (A5861; Abclonal), c-Myc (GTX103436; GeneTex), β-actin (sc-47778; Santa Cruz Biotechnology). Antibodies used for immunohistochemistry: USP16 (HPA021140; Sigma-Aldrich), Ki67 (sc-15402; Santa Cruz), c-Myc (#ab32072, Abcam). Antibodies used for immunoprecipitation and immuno uorescence: Flag (#30503ES60, Yeason), USP16 (HPA021140; Sigma-Aldrich), c-Myc (13987S, Cell Signaling Technology).
Ge J., Yu W., Li J., Ma H., Wang P., Zhou Y., Wang Y., Zhang J., & Li X. (2020). USP16 Regulates Castration-Resistant Prostate Cancer Cell Proliferation by Deubiquitinating and Stablizing c-Myc.
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