Primary antibody against ha

Manufactured by Roche

The primary antibody against HA is a laboratory reagent used for the detection and identification of proteins tagged with the influenza hemagglutinin (HA) epitope. This antibody specifically binds to the HA tag, allowing researchers to track the expression and localization of HA-tagged proteins in various experimental systems.

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Lab products found in correlation

Alexa fluor 488 conjugated anti mouse secondary antibody, by Thermo Fisher Scientific (2 mentions) Alexa fluor 568 conjugated anti rabbit secondary antibody, by Thermo Fisher Scientific (2 mentions) Aurora b, by Cell Signaling Technology (1 mentions) Fluorescence microscope, by Leica (1 mentions) Fluorescence microscope, by Nikon (1 mentions)

Close spelling variants of this product

An antibody against HA (2 citations)

2 protocols using primary antibody against ha

Visualization of HPV16 E6 and E7 Localization

Cited 1 time

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This assay is carried out as described previously [30 (link)]. Approximately 2 × 10⁵ U-2 OS cells were plated onto coverslips. The cells were either mock-transfected or transfected with HA-tagged 16 E6 or E7. Cells were fixed with ice-cold absolute methanol 24 h after transfection and incubated with primary antibody against HA (Roche) and Aurora B (Cell Signaling), followed by Alexa Fluor^® 568-conjugated anti-rabbit secondary antibody and Alexa Fluor^®488-conjugated anti-mouse secondary antibodies (ThermoFisher Scientific), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI). The subcellular location of HPV16 E6 or E7 and AurB were examined under a fluorescence microscope (Leica, Wetzlar, Germany).

Boon S.S., Lee Y.C., Yip K.L., Luk H.Y., Xiao C., Yim M.K., Chen Z, & Chan P.K. (2023). Interaction between Human Papillomavirus-Encoded E6 Protein and AurB Induces Cell Immortalization and Proliferation—A Potential Target of Intervention. Cancers, 15(9), 2465.

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Subcellular Localization of HPV16 E6 and AurA

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This assay is carried out as described previously (29 (link)). Approximately 2 × 10⁵ U-2 OS cells were plated onto coverslips. The cells were transfected with HA-tagged 16 E6 and Flag-tagged AurA. Cells were fixed with ice-cold absolute methanol 24 h after transfection and incubated with primary antibody against HA (Roche), Flag (Santa Cruz Biotechnology), and AurA (Cell Signaling), followed by Alexa Fluor 568-conjugated anti-rabbit secondary antibody and Alexa Fluor 488-conjugated anti-mouse secondary antibodies (Thermo Fisher Scientific), and counterstained in 4′,6-diamidino-2-phenylindole (DAPI). The subcellular location of HPV16 E6 and AurA were examined under a fluorescence microscope (Nikon).

Li S., Yim M.K., Yip K.L., Xiao C., Luk H.Y., Xiao S., Chen Z., Chan P.K, & Boon S.S. (2023). E6-Encoded by Cancer-Causing Human Papillomavirus Interacts with Aurora Kinase A To Promote HPV-Mediated Carcinogenesis. Journal of Virology, 97(2), e01872-22.

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