Prism v5

Manufactured by GraphPad

Sourced in United States, United Kingdom, Canada, Belgium

GraphPad Prism v5.0 is a data analysis and graphing software used for scientific research. It provides tools for statistical analysis, curve fitting, and creating publication-quality graphs and charts.

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Lab products found in correlation

Spss v22, by IBM (17 mentions) Spss v17, by IBM (14 mentions) Spss v20, by IBM (12 mentions) Sas version 9, by SAS Institute (10 mentions) Spss v21, by IBM (10 mentions) Spss v16, by IBM (9 mentions) Spss v23, by IBM (8 mentions) Spss v18, by IBM (8 mentions) Spss 22, by IBM (8 mentions) Spss 19, by IBM (7 mentions) Spss statistics v21, by IBM (6 mentions) Dnase 1, by Thermo Fisher Scientific (5 mentions) Sas 9, by SAS Institute (5 mentions) Sas v9, by SAS Institute (5 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (5 mentions) Spss v15, by IBM (5 mentions) Prism 5, by GraphPad (5 mentions) Spss version 22, by IBM (5 mentions) Cfx96 system, by Bio-Rad (4 mentions) Spectramax id3, by Molecular Devices (4 mentions)

Close spelling variants of this product

Prism Software v5 (120 citations) Prism v5.0a (34 citations) Prism v5.0d (30 citations) Prism 5 V5.01 (26 citations) Prism v5.0b (23 citations) GraphPad Prism v5.0c (19 citations) GraphPad Prism v5.00 for Windows (19 citations) GraphPad Prism v5.0f (5 citations) GraphPad Prism v5.02 for Windows (3 citations) Prism v5.0c software (3 citations)

2 615 protocols using prism v5

Pathological Response to Neo-adjuvant Chemotherapy

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Response to Neo-adjuvant Chemotherapy (NACT) was calculated by comparing clinical tumor size (cT) and lymph node status (cN) to pathological tumor size (ypT) and pathological lymph node status (ypN). Post-NACT pathological status if yPT0 (no residual tumor) or ypTis (residual DCIS) and no lymph node involvement (ypN0), the response was defined as pathological Complete Response (pCR). For patients with residual tumor (ypT1-ypT4) and/or lymph node metastasis (ypN1–3) in a post-NACT setting, the response was noted as Residual Disease (RD).
Response to NACT across the subtypes is then analyzed using a 3 × 4 Chi-square contingency test. Box plot for percent sTILs scores according to the response to NACT, i.e., pCR and RD, is plotted using GraphPad Prism v.5. Mean sTILs with S.E. according to the response to NACT for each subtype is computed and plotted by using GraphPad Prism v.5.
sTILs scores of primary biopsy and post-treatment surgery tissues were plotted using a before-after graph using GraphPad Prism v.5. Paired t-test was performed to assess the significance of the difference in mean sTILs scores between primary and post-NACT tumor tissue.

Vaid P.M., Puntambekar A.K., Jumle N.S., Banale R.A., Ansari D., Reddy R.R., Unde R.R., Namewar N.P., Kelkar D.A., Shashidhara L.S., Koppiker C.B, & Kulkarni M.D. (2022). Evaluation of tumor-infiltrating lymphocytes (TILs) in molecular subtypes of an Indian cohort of breast cancer patients. Diagnostic Pathology, 17, 91.

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Survival Analysis of Infected Larvae

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Survival data were plotted using the Kaplan–Meier method and comparisons between groups were made using the log-rank test using GraphPad Prism v 5.03. In all comparisons with the negative control, it was the uninfected control (rather than the un-manipulated control) that was used. In all tests, P ≤ 0.05 was considered significant. The lethal dose leading to 50% mortality (LD50) at different time points was determined by plotting the percentage of surviving larvae following infection with different inocula, and non-linear curve fitting using GraphPad Prism v 5.03.

Velikova N., Kavanagh K, & Wells J.M. (2016). Evaluation of Galleria mellonella larvae for studying the virulence of Streptococcus suis. BMC Microbiology, 16, 291.

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Experimental Autoimmune Encephalomyelitis and Collagen-Induced Arthritis Analysis

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The data were analyzed with Microsoft Excel (descriptive statistics) and Statistica v.7.1 for Windows. The data on EAE were confirmed with the Kruskal–Wallis and Kholmogorov–Smirnov tests at p < 0.05 and then further analyzed with one-way ANOVA (HSD) at p < 0.05. A post hoc Tukey’s honestly significant difference procedure performed pairwise comparisons using the ANOVA data set. The F statistic accounted for the overall differences among the sample (n) means (Mn). Tukey’s HSD test determined if a significant difference between the various pairs of means was possible at the chosen significant level. The data on the CIA model were analyzed for two sexes separately. The repeated results were analyzed with a single-factor dispersion analysis (ANOVA). The quantitative results of the disability test were evaluated with non-parameter Mann–Whitney criteria (U-test). Illustrations were produced with GraphPad «Prism v.5 and Prism v.5.04» software. The data values displayed mean ± SEM values; the volume of each group of animals used in the EAE experiment was n = 6; the volume of each group used in the CIA experiment was n = 8.

Danilkovich A.V., Turobov V.I., Palikov V.A., Palikova Y.A., Shepelyakovskaya A.O., Mikhaylov E.S., Slashcheva G.A., Shadrina T.E., Shaykhutdinova E.R., Rasskazova E.A., Tukhovskaya E.A., Khokhlova O.N., Dyachenko I.A., Ismailova A.M., Zinchenko D.V., Navolotskaya E.V., Lipkin V.M., Murashev A.N, & Udovichenko I.P. (2023). C-Terminal Region of Caveolin-3 Contains a Stretch of Amino Acid Residues Capable of Diminishing Symptoms of Experimental Autoimmune Encephalomyelitis but Not Rheumatoid Arthritis Modeled in Rats. Biomedicines, 11(10), 2855.

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Survival Analysis of Experimental Treatments

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Statistical analysis was performed using GraphPad Prism v5.04. Survival analysis was conducted using Kaplan-Meier plots with log-rank tests. Other analyses were performed using unpaired Student's t-test (except where results were normalized to an internal control, in which case a paired t-test was used) or 1-way ANOVA as appropriate. Bonferroni post-tests were used to compare individual groups. For data with a non-normal distribution, Mann-Whitney and Kruskal-Wallis tests with Dunn's post-test were used instead. Results with p < 0.05 were considered significant, and are indicated on graphs according to the reporting summary in Prism v5.04, whereby * = p < 0.05 but > 0.01; ** = p < 0.01 but > 0.001 and *** = p < 0.001.

Tan L.Y., Mintoff C., Johan M.Z., Ebert B.W., Fedele C., Zhang Y.F., Szeto P., Sheppard K.E., McArthur G.A., Foster-Smith E., Ruszkiewicz A., Brown M.P., Bonder C.S., Shackleton M, & Ebert L.M. (2016). Desmoglein 2 promotes vasculogenic mimicry in melanoma and is associated with poor clinical outcome. Oncotarget, 7(29), 46492-46508.

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Analyzing EZH2 Regulatory Targets

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Throughout the current study, two tailed paired Student t-test and One-way ANOVA was performed to test the statistical difference between the experimental groups using the software GraphPad Prism v5.01. In addition, correlation coefficient (r) between EZH2 and its targets genes was calculated with the raw matrix data provided in MERAV database using GraphPad Prism v5.01.

Kumari K., Das B., Adhya A.K., Rath A.K, & Mishra S.K. (2019). Genome-wide expression analysis reveals six contravened targets of EZH2 associated with breast cancer patient survival. Scientific Reports, 9, 1974.

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Saturation Binding of CRF1 Receptor Variants

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For saturation binding experiments, membranes were prepared from HEK293T cells transiently expressing wild-type CRF₁R, CRF₁R StaR, CRF₁R StaR with T4L fusion, or CRF₁R-#105 as described36 (link) and incubated in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% (w/v) polyethylinimine (PEI) with [³H]CP-376395 (0–60 nM) in the presence or absence of 30 μM cold CP-376395. Final DMSO concentration in each reaction was 5% (v/v). Membranes were incubated for 18 hours at room temperature prior to rapid filtration through 96-well GF/C UniFilter plates pre-soaked in 0.3% (w/v) PEI, followed by washing with PBS containing 0.15% (w/v) CHAPS. Plates were dried, 50 μl Ultima Gold-F scintillation fluid added per well and bound ligand measured using a Packard Microbeta counter. To obtain K_d, data were analysed using a global fitted one-site binding hyperbola in GraphPad Prism v5. Heterologous competition analysis was conducted as above, incubating each construct with K_d concentrations of radioligand and a dilution series of competing ligand. To obtain K_i, data were analysed using a globally fitted one-site fit K_i analysis in GraphPad Prism v5.01.

Kean J., Bortolato A., Hollenstein K., Marshall F.H, & Jazayeri A. (2015). Conformational thermostabilisation of corticotropin releasing factor receptor 1. Scientific Reports, 5, 11954.

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ELISA-based ERBB3 Serum Quantification

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The ELISA standard curve was plotted using Prism v5 (GraphPad, CA, USA) and results for each serum interpolated and corrected for dilution. The significance of differences in serum ERBB3 between groups was determined using a Kruskal-Wallis test with Dunn’s Multiple Comparison utilizing Prism v5 (GraphPad, CA, USA).

Hayes D.A., Kunde D.A., Taylor R.L., Pyecroft S.B., Sohal S.S, & Snow E.T. (2017). ERBB3: A potential serum biomarker for early detection and therapeutic target for devil facial tumour 1 (DFT1). PLoS ONE, 12(6), e0177919.

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Analyzing Malaria Antibody and Cytokine Responses

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Figures and statistical analyses were generated in R version 3.5.1 and GraphPad Software (Prism V5). Wilcoxon rank sum test or Mann–Whitney test were used to compare continuous variables. Chi-square test was used to compare categorical variables. Absolute values for antibody titers and concentrations of cytokines and chemokines were plotted. Data were log transformed only when investigating the anti-PfCSP antibody titres and viremia levels. Spearman correlation was used to investigate the potential effect of HPgV-1 infection status and viremia with antibody titres and cytokine levels. Data for cytokines, chemokines and growth factors were not analysed for multiple correction as we considered this question as exploratory. P value ≤ 0.05 was considered significant. Differences in viral diversity, abundance and prevalence were assessed using Linear discriminant analysis effect size [45 (link)] and GraphPad Software (Prism V5), respectively.

Tumbo A.M., Schindler T., Dangy J.P., Orlova-Fink N., Bieri J.R., Mpina M., Milando F.A., Juma O., Hamad A., Nyakarungu E., Chemba M., Mtoro A., Ramadhan K., Olotu A., Makweba D., Mgaya S., Stuart K., Perreau M., Stapleton J.T., Jongo S., Hoffman S.L., Tanner M., Abdulla S, & Daubenberger C. (2021). Role of human Pegivirus infections in whole Plasmodium falciparum sporozoite vaccination and controlled human malaria infection in African volunteers. Virology Journal, 18, 28.

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Statistical Analysis of Box-and-Whisker Plots

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Box-and-whisker graphs were plotted with Prism v5.0 (GraphPad Software). For all graphs, the top and bottom of the box corresponds to the 25th and 75th percentile (the lower and upper quartiles, respectively) and the line near the middle of the box marks the median (50th percentile).Whiskers correspond to the 5–95 percentiles. Data not included between the whiskers are plotted as outliers (dots). Statistical analysis was performed in Prismv5.0 (GraphPad Software) using the non-parametric Mann–Whitney rank sum test.

Chaib-Mezrag H., Lemaçon D., Fontaine H., Bellon M., Bai X.T., Drac M., Coquelle A, & Nicot C. (2014). Tax impairs DNA replication forks and increases DNA breaks in specific oncogenic genome regions. Molecular Cancer, 13, 205.

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Plasma Lipid and Inflammatory Biomarker Profiling

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Blood samples were collected after an overnight fast and processed. Plasma were stored at −80 °C until further analyses. Total cholesterol, LDL-c, HDL-c, Lp(a), triglycerides, and transaminases were measures by certified enzymatic techniques. PCSK9 plasma levels were evaluated through a commercial ELISA kit (Human Proprotein Convertase 9/PCSK9, Quantikine® ELISA SixPak, R&D System, MN, USA, cod. SPC900), according to manufacturer's instructions. The assay sensitivity was 0.219 ng/mL, while intra- and inter-precision assay were 5.4 ± 1,2% and 4.8 ± 1.0%, respectively. PCSK9 sample concentrations were obtained by generating a four parametric logistic curve-fit (GraphPad Prism v5.0). High-sensitivity CRP was measured by a commercial ELISA kit (hsCRP ELISA, apDia, Belgium, cod. 740011), according to manufacturer's instructions. The minimal detectable concentration is approximately 0.02 μg/mL. Intra-assay variability was 5.1 ± 1.6%, inter-assay variability was 6.1 ± 0.3%. A linear curve-fit (GraphPad Prism v5.0) was used to obtain hsCRP concentrations. A panel of pro and anti-inflammatory cytokines (ProcartaPlex Human Cytokine Panel 1B 25plex, ThermoFisher, MA, USA, cod. EPX250-12166-901) were determined by Luminex-xMAP® technology from plasma samples, according to manufacturers' instructions.

Lupo M.G., Arcidiacono D., Zaramella A., Fimiani F., Calabrò P., Cefalù A.B., Averna M., D'Erasmo L., Arca M., De Martin S., Zambon A, & Ferri N. (2021). Lomitapide does not alter PCSK9 and Lp(a) levels in homozygous familial hypercholesterolemia patients: Analysis on cytokines and lipid profile. Atherosclerosis Plus, 43, 7-9.

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