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Anti cd3 bv711

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Anti-CD3 BV711 is a fluorescently-labeled antibody that binds to the CD3 protein expressed on the surface of T cells. It can be used for the identification and analysis of T cells in flow cytometry applications.

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Lab products found in correlation

Anti cd69 pe, by BioLegend (2 mentions) Anti cd28 apc, by BioLegend (2 mentions) Anti cd45ro bv711, by BioLegend (2 mentions) Anti cxcr3 fitc, by BioLegend (2 mentions) Anti ccr4 bv421, by BioLegend (2 mentions) Anti ccr6 bv605, by BioLegend (2 mentions) Anti ccr7 bv650, by BioLegend (2 mentions) Anti cd14 pe, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Anti cd56 alexafluor700, by BioLegend (1 mentions) Anti cd4 pe cy5, by BioLegend (1 mentions) Anti cd8 bv605, by BioLegend (1 mentions) Anti cd19 percp cy5, by BioLegend (1 mentions) Anti cd16 bv785, by BioLegend (1 mentions) Anti cd45ra pe cy7, by BioLegend (1 mentions) Anti ccr7 alexa fluor 647, by BD (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Anti cd19 pe, by BioLegend (1 mentions) Anti cd27 pe, by BioLegend (1 mentions) Anti cd45 bv711, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-CD3 (468 citations) CD3-BV711 (11 citations)

4 protocols using anti cd3 bv711

1

Multiparametric Flow Cytometry Analysis of Immune Cell Subsets

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The subsets of T lymphocytes14 (link),15 (link) and monocytes12 (link),13 (link) at baseline and at following timepoints during PU-H71 treatment were monitored by flow cytometry. Peripheral blood (PB) cells from healthy donor was used as a control. Cells were isolated by the Ficoll-Paque method from PB and bone marrow (BM) aspirates. Cells were labeled with anti-CD45 APC-H7, anti-CD33 BV650 (BD Biosciences, clone WM53, cat. 303430), anti-CD56 AlexaFluor700 (BioLegend, clone HCD56, cat. 318316), anti-CD3 BV711 (BioLegend, clone SK7, cat. 344838), anti-CD4 PE-Cy5 (BioLegend, clone OKT4, cat. 317412), anti-CD8 BV605 (BioLegend, clone SK1, cat. 344742), anti-CD19 PerCP/Cy5.5 (BioLegend, clone HIB19, cat. 302230), anti-CD14 PE (BD Biosciences, clone M5E2, cat. 555398), anti-CD16 BV785 (BioLegend, clone 3G8, cat. 302046), anti-CD45RA PE-Cy7 (BioLegend, clone HI100, cat. 304126) and anti-CCR7 Alexa Fluor 647 (BD Biosciences, clone 150503, cat. 560816). After washing, flow cytometry evaluation was performed on BD LSRFortessa. Data was analyzed using FlowJo software.
Sugita M., Wilkes D.C., Bareja R., Eng K.W., Nataraj S., Jimenez-Flores R.A., Yan L., De Leon J.P., Croyle J.A., Kaner J., Merugu S., Sharma S., MacDonald T.Y., Noorzad Z., Panchal P., Pancirer D., Cheng S., Xiang J.Z., Olson L., Van Besien K., Rickman D.S., Mathew S., Tam W., Rubin M.A., Beltran H., Sboner A., Hassane D.C., Chiosis G., Elemento O., Roboz G.J., Mosquera J.M, & Guzman M.L. (2021). Targeting the epichaperome as an effective precision medicine approach in a novel PML-SYK fusion acute myeloid leukemia. NPJ Precision Oncology, 5, 44.
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2

Jurkat T Cell Phenotyping by Flow

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Jurkat T cells were stimulated under indicated conditions. About half a million cells per condition were harvested, washed and incubated with fluorophore-conjugated antibodies for 20 min at room temperature in MACS buffer (DBPS without calcium and magnesium, supplemented with 1% FBS). Cells were washed and resuspended in MACS buffer and analyzed by flow cytometry in corresponding fluorescent channels relative to unstained control. Antibodies used: anti-CD69 PE, anti-CD28 APC, anti-CD3 BV711, anti-CD45RO BV711, anti-CXCR3 FITC, anti-CCR4 BV421, anti-CCR6 BV605, and/or anti-CCR7 BV650 antibodies (all diluted 1:100 and from Biolegend).
Agosto L.M., Mallory M.J., Ferretti M.B., Blake D., Krick K.S., Gazzara M.R., Garcia B.A, & Lynch K.W. (2023). Alternative splicing of HDAC7 regulates its interaction with 14-3-3 proteins to alter histone marks and target gene expression. Cell reports, 42(3), 112273.
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3

Jurkat T Cell Phenotyping by Flow

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Jurkat T cells were stimulated under indicated conditions. About half a million cells per condition were harvested, washed and incubated with fluorophore-conjugated antibodies for 20 min at room temperature in MACS buffer (DBPS without calcium and magnesium, supplemented with 1% FBS). Cells were washed and resuspended in MACS buffer and analyzed by flow cytometry in corresponding fluorescent channels relative to unstained control. Antibodies used: anti-CD69 PE, anti-CD28 APC, anti-CD3 BV711, anti-CD45RO BV711, anti-CXCR3 FITC, anti-CCR4 BV421, anti-CCR6 BV605, and/or anti-CCR7 BV650 antibodies (all diluted 1:100 and from Biolegend).
Agosto L.M., Mallory M.J., Ferretti M.B., Blake D., Krick K.S., Gazzara M.R., Garcia B.A, & Lynch K.W. (2023). Alternative splicing of HDAC7 regulates its interaction with 14-3-3 proteins to alter histone marks and target gene expression. Cell reports, 42(3), 112273.
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4

Comprehensive CAR Expression Analysis

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For evaluation of CAR expression, cells were stained with a goat anti-mouse Fab antibody conjugated with Alexa Fluor 647 (Jackson ImmunoResearch) or biotinylated CD19-Fc (ACRO Biosystems) for 15 min at room temperature. Cells were thoroughly washed before staining with antibodies for additional surface markers. Anti-CD3-BB515 and anti-CD62L-BB515 were purchased from BD Biosciences. Anti-CD4-BV785, anti-CD8-BV711, anti-CD45RA-BV421, anti-CD45RO-PECy7, streptavidin-PE, anti-CD27-PE, anti-CD95-allophycocyanin (APC)Cy7, anti-CCR7-APC, anti-CD45-BV711, and anti-CD19-PE, anti-CD3-BV711 were purchased from BioLegend and used to stain surface antigens. Cell Trace Violet was purchased from Thermo Fisher Scientific and used at a concentration of 1 μM to label cells for 10 min. All data were acquired using a BD Fortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software.
MacLeod D.T., Antony J., Martin A.J., Moser R.J., Hekele A., Wetzel K.J., Brown A.E., Triggiano M.A., Hux J.A., Pham C.D., Bartsevich V.V., Turner C.A., Lape J., Kirkland S., Beard C.W., Smith J., Hirsch M.L., Nicholson M.G., Jantz D, & McCreedy B. (2017). Integration of a CD19 CAR into the TCR Alpha Chain Locus Streamlines Production of Allogeneic Gene-Edited CAR T Cells. Molecular Therapy, 25(4), 949-961.
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