Vacutainer blood collection tube

Secondary outcomes of the study were high-risk human papillomavirus (HPV) positivity after the 3-month follow-up visit and the biomarkers of inflammation (high-sensitive C-reactive protein, fibrinogen and sedimentation). Detection of the presence of high-risk HPV in cervical smears was made using the Hybrid Capture II HPV Test (Qiagen, Germantown, MD, USA). The samples were tested for the presence of HRHPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68. For the assessment of other secondary outcomes blood samples were collected before and after the treatment at three months in the experimental group by venipuncture after a 10 h fasting period. Blood was placed in siliconized test tubes without anticoagulant for biochemical determinations. The blood sedimentation rate was measured according to standard protocol in BD Vacutainer^® blood collection tubes (Becton, Dickinson and Company, New York, USA). Fibrinogen was determined by standard methodology, using an automated hemostasis analyzer (BCS^® XP System, Siemens, Marburg, Germany). High-sensitive CRP was determined by the immunoturbidimetric method using Multigent CRP Vario^® reagent on Architect ci8200 integrated system (Abbott, Chicago, IL, USA)

The rats were fasted overnight prior to termination. On the day of termination 15 mg/kg of sodium pentobarbital was injected intraperitoneally. An incision was performed, and the rats were killed by exsanguination. Blood samples were collected in BD vacutainer blood collection tubes (Becton Dickinson Pty Ltd, Johannesburg, South Africa) for clinical biochemistry analysis; and the livers were collected, washed in saline solution, weighed, and fixed in 10% formalin for histology. Parts of the liver were snap frozen and stored at − 80 °C for further analysis.

Peripheral venous blood samples were collected before biopsy in 6 mL EDTA-containing BD™ Vacutainer^® Blood Collection Tubes (Becton Dickinson, NJ, USA) and immediately processed into plasma by dual centrifugation (at 1400× g and 4500× g, both for 10 min at room temperature). All patients included in the study provided blood samples.
Semen samples were obtained by masturbation after 3–5 days of sexual abstinence, one day before the biopsy, and processed after 30–60 min liquefaction at room temperature into seminal plasma by dual centrifugation (at 400× g and 12000× g, both for 10 min at room temperature). All fractions were subsequently stored at −80 °C until analysis. All further analysis was performed on the Department of Medical Biology at the University of Zagreb School of Medicine.