Precision xtra glucose and ketone monitoring system

To investigate the effect of KSMCT on blood βHB and glucose levels we measured them on the last (third) control day (control), on the days of the KSMCT gavage (fourth day of experiments) and on the PTC day (fifth day of experiments) after EEG measurements (group 7) by the Precision Xtra™ glucose and ketone monitoring system (Abbott Laboratories, Abbott Park, IL, USA). As the device is able to detect only R-βHB level, the measured βHB level would be lower than the total blood ketone body level (R-βHB + L-βHB + AcAc + acetone). Although the Precision Xtra™ is R-βHB specific, it remains to be determined in a separate, later study whether the use of racemic mixture exogenous ketones is inferior to enantiomerically pure substances (e.g., competition between R and L forms is plausible). Blood, which was used for detection of R-βHB (mmol/L) and glucose (mg/dl) levels, was taken from the tail veins [25 (link),41 (link)] (group 7).

Blood β-hydroxybutyrate levels were measured using a Precision Xtra glucose and ketone monitoring system (Abbott, Chicago, IL). Blood was obtained via a tail nick in both the fasted (immediately prior to AM or PM meal) or fed (3 hours post-prandial) animals. Measurements were taken 2, 4 and 6 months after initiation of diet from TgF344-AD animals in the 12-month-old cohorts.