A8481

Manufactured by Merck Group

A8481 is a piece of laboratory equipment produced by Merck Group. It serves as a tool for scientific research and analysis, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. The available information is limited, and any further interpretation or extrapolation would go beyond the scope of this request.

Automatically generated - may contain errors

Lab products found in correlation

Anti β actin, by Merck Group (1 mentions) Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Ab56416, by Abcam (1 mentions) Ab108327, by Abcam (1 mentions) Ab48394, by Abcam (1 mentions) Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (1 mentions) Ipvh00010, by Merck Group (1 mentions) Ab75273, by Abcam (1 mentions) Ab38560, by Abcam (1 mentions) Ab185230, by Abcam (1 mentions)

2 protocols using a8481

Western Blot Analysis of Autophagy Markers

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Tissue samples and cultured cells were lysed with RIPA lysis buffer containing protease inhibitor. SDS-PAGE was used to detect the proteins of interest. Proteins were resolved on SDS gels and were electro-transferred to polyvinylidene fluoride membranes (Millipore, Boston, MA, USA). Then, the membranes were blocked with 5% non-fat milk and incubated overnight at 4°C with primary antibodies, including anti-LC3B (#ab48394, 1:500; Abcam), anti-p62 (#ab56416, 1:500; Abcam), anti-ATG5 (#ab108327, 1:500; Abcam), anti-BECLIN1 (#ab55877, 1:500; Abcam), and anti-β-ACTIN (#A8481, 1:4000; Sigma-Aldrich). After washing, the membranes were incubated with a secondary antibody and imaged with Odyssey SA (Gene Company Limited, Hong Kong, China).

Yu X., Ye X., Lin H., Feng N., Gao S., Zhang X., Wang Y., Yu H., Deng X, & Qian B. (2018). Knockdown of long non-coding RNA LCPAT1 inhibits autophagy in lung cancer. Cancer Biology & Medicine, 15(3), 228-237.

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Protein Expression in Achilles Tendon Tissues

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Achilles tendon tissues of mice post surgery were obtained and grinded with glass pestles in RIPA buffer on ice before ultrasonication for tissue protein extraction. Cell lysates were collected as described above. Samples were fractionated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (IPVH00010, Millipore). Immunoblotting was performed with primary antibodies against following proteins: Sox9 (1:2000; ab185230, Abcam), Runx2 (1:2000; NBP1-77461, Novusbio), FGFR3 (1:2000; BS90509, bioworld), BMPR1a (1:1000; ab38560, Abcam), pSmad1/5 (1:500; 700047, Thermo), Smad1/5 (1:1000; ab75273, Abcam), β-actin (1:5000; A8481, Sigma-Aldrich). Chemiluminescence was performed with SuperSignal West Dura Extended Duration Substrate (Thermo).

Zhang D., Huang J., Sun X., Chen H., Huang S., Yang J., Du X., Tan Q., Luo F., Zhang R., Zhou S., Jiang W., Ni Z., Wang Z., Jin M., Xu M., Li F., Chen L., Liu M., Su N., Luo X., Yin L., Zhu Y., Feng J.Q., Chen D., Qi H., Chen L, & Xie Y. (2021). Targeting local lymphatics to ameliorate heterotopic ossification via FGFR3-BMPR1a pathway. Nature Communications, 12, 4391.

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