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Rt2 qpcr primer assays for human gapdh and abat

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The RT2 qPCR Primer Assays for Human GAPDH and ABAT are pre-designed, gene-specific primer assays for use in real-time quantitative PCR (qPCR) experiments. The GAPDH assay targets the Glyceraldehyde-3-Phosphate Dehydrogenase gene, and the ABAT assay targets the 4-Aminobutyrate Aminotransferase gene. These assays are intended for research use only and provide a convenient solution for gene expression analysis.

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Lab products found in correlation

Steponeplus rt pcr system, by Thermo Fisher Scientific (2 mentions) Perfecta sybr green fastmix rox, by Quanta Biosciences (2 mentions)

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RT2 qPCR Primer Assays (110 citations) GAPDH primers (17 citations) QPCR primers (12 citations) RT2 qPCR assays (6 citations) RT2 Primer Assays (3 citations) RT2 qPCR Primer Assay for Human GAPDH (2 citations) RT2 qPCR Primer Assay primers (2 citations) QPCR Primer Assays (2 citations) Primers for GAPDH (2 citations) Human GAPDH (2 citations)

2 protocols using rt2 qpcr primer assays for human gapdh and abat

1

Quantitative RT-PCR Analysis of GAPDH and ABAT

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RNA was extracted and cDNA synthesized using standard protocols. Quantitative realtime PCR experiments were performed using a StepOne Plus RT-PCR system (Applied Biosystems), RT2 qPCR Primer Assays for Human GAPDH and ABAT (SABiosciences) and PerfeCTa SYBR Green FastMix ROX (Quanta Biosciences). The cycle of threshold value (Ct) was normalized to the transcripts for the housekeeping gene GAPDH. All assays were conducted in triplicate and the results are shown as the average and standard deviation.
Besse A., Wu P., Bruni F., Donti T., Graham B.H., Craigen W.J., McFarland R., Moretti P., Lalani S., Scott K.L., Taylor R.W, & Bonnen P.E. (2015). The GABA transaminase, ABAT, is essential for mitochondrial nucleoside metabolism. Cell metabolism, 21(3), 417-427.
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2

Quantitative Real-Time PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted and cDNA synthesized using standard protocols. Quantitative real-time PCR experiments were performed using a StepOne Plus RT-PCR system (Applied Biosystems), RT2 qPCR Primer Assays for Human GAPDH and ABAT (SABiosciences) and PerfeCTa SYBR Green FastMix ROX (Quanta Biosciences). All assays were conducted in triplicate and the results are shown as the average and standard deviation.
Besse A., Petersen A.K., Hunter J.V., Appadurai V., Lalani S.R, & Bonnen P.E. (2016). Personalized medicine approach confirms a milder case of ABAT deficiency. Molecular Brain, 9, 93.
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