RosaNotchICD floxed mice (Murtaugh et al., 2003 (link)) purchased from Jackson Laboratories (Bar Harbor, ME, USA) and Rbpj floxed mice (Dr. Tasuku Honjo, Han et al., 2002 (link)) were bred to Nkx2.1-cre mice (Lazzaro et al., 1991 (link), Xu et al., 2008 (link)) purchased from Jackson Laboratories. Hes1 mutant mice were previously generated by replacing the first 3 exons with a neomycin-resistance cassette (Ishibashi, et al. 1994; gift from Dr. Kageyama). Breeding colonies were generated at the University of Illinois at Urbana-Champaign (UIUC) and all animal procedures were approved by the UIUC Institutional Animal Care and Use Committee. Genotyping was performed as described previously (Lazzaro et al., 1991 (link), Han et al., 2002 (link), Murtaugh et al., 2003 (link)).
Nkx2.1 cre mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
Nkx2.1-Cre mice are a type of genetically modified mouse that express the Cre recombinase enzyme under the control of the Nkx2.1 gene promoter. The Nkx2.1 gene is expressed in specific regions of the developing brain, thyroid, and lung. These mice can be used to study the role of genes or cellular processes in the development and function of these tissues.
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Lab products found in correlation
6 protocols using nkx2.1 cre mice
1
Genetic Mouse Models for Notch Signaling
2
Generating Pde3b Knockout Mice
3
Mouse Models of Lung Injury
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C57BL/6J mice were purchased from Beijing Huafukang Bioscience. Nkx2.1-Cre mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Atg5f/f mice were originally obtained from RIKEN BRC through the National Bio-Resource Project of the MEXT in Japan. Nkx2.1-Cre mice were crossed with Atg5f/f mice to generate Nkx2.1Cre;Atg5f/f mice. SftpcCreER mice were purchased from The Jackson Laboratory (stock no. 028054). Atg5f/f mice were crossed with SftpcCreER mice to generate SftpcCreER;Atg5f/f mice. Glut1f/f mice, generated as described previously (Young et al., 2011 (link)), were crossed with SftpcCreER mice to generate SftpcCreER;Glut1f/f mice. Tamoxifen was injected intraperitoneally at 50 mg/kg once daily for 5 days to induce knockdown of Atg5 or Glut1 in mouse AT2 cells. All mice were housed in a specific pathogen-free facility at Tianjin University Haihe Hospital. All mice were exposed to an alternating 12-h light/dark cycle and had free access to food and water. Age- and sex-matched mice were randomly grouped. Eight- to 12-week-old male mice were used in BLM-induced lung injury experiments. All mice were treated with strict adherence to the protocol (no. 2015HHLL06) approved by the Tianjin University Haihe Hospital Animal Care and Use Committee.
4
Diet and Genetic Manipulation in Mice
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Mice were housed in a specific pathogen–free animal facility, fed Picolab diet 5053 (Purina, Richmond, IN) or D124921 60% high-fat diet (HFD) (Research Diets, New Brunswick, NJ), and kept under a 12-h light/dark cycle. Nkx2.1-Cre mice were obtained from The Jackson Laboratory (Bar Harbor, ME) and backcrossed with mice having a C57BL/6J background for eight generations. Mice with loxP-flanked FoxO1 alleles have been previously described (22 (link)). All procedures were approved by the institutional animal care and utilization committee at Columbia University.
5
Mapping Nkx2.1+ Progenitor Fate
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The following mice were used: ICR wild type (Nihon SLC), Nkx2.1Cre mice (Stock no. 008661, Jackson Laboratory) and Ai9 mice (Stock no. 007905, Jackson Laboratory). These transgenic mice were backcrossed onto the ICR background. Nkx2.1Cre mice were crossed with Ai9 mice to fate map Nkx2.1+ progenitors. The noon of vaginal plug detection was designated as embryonic day 0.5 (E0.5). The day of birth was designated as postnatal day 0 (P0).
EdU or BrdU was intraperitoneally injected at E10.5, E12.5, E14.5 or E16.5 in some of the animals. Although these injections were made for another purpose, data from these animals were also included in the analysis. We found no difference in gross anatomy or in the distribution of labeled cells between injected and un-injected animals.
All experiments involving mouse care, surgery and sample preparation were approved by the Animal Experimental Committee of Osaka University Graduate School of Frontier Biosciences and performed in accordance with the NIH guidelines and the Osaka University Guidelines for the Welfare and Use of Laboratory Animals.
EdU or BrdU was intraperitoneally injected at E10.5, E12.5, E14.5 or E16.5 in some of the animals. Although these injections were made for another purpose, data from these animals were also included in the analysis. We found no difference in gross anatomy or in the distribution of labeled cells between injected and un-injected animals.
All experiments involving mouse care, surgery and sample preparation were approved by the Animal Experimental Committee of Osaka University Graduate School of Frontier Biosciences and performed in accordance with the NIH guidelines and the Osaka University Guidelines for the Welfare and Use of Laboratory Animals.
6
VGluT3+ and Nkx2.1+ Interneuron Targeting
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All experiments were conducted in accordance with animal protocols approved by the National Institutes of Health (ASP# 17–045). VGluT3+ cells were genetically accessed and targeted using Tg(Slc17a8-icre)1Edw/SealJ mice (referred as VGluT3-Cre mice, Grimes et al., 2011 (link)), The Jackson Laboratory, stock #018147). Medial ganglionic eminence derived interneurons were targeted using Tg(Nkx2-1-cre)2Sand/J (referred to as Nkx2.1-Cre mice, The Jackson Laboratory, stock #008661). Male and female offspring from crosses with Cre-activated reporter lines conditionally expressing either tdTomato (Ai14 mice, The Jackson Laboratory, stock #007908) or ChR2-YFP (Ai32, The Jackson Laboratory, stock #012569) were used from P5 to 1 year as indicated. In addition, we used C57BL/6J wild-type mice for ISH and IHC studies that did not require transgenic reporting. Mice were housed and bred in a conventional vivarium with standard laboratory chow (or Teklad TD.01270 Pyridoxine Deficient Diet (B6-,~0.1 ppm) or Teklad TD. TD.01271 control diet with Pyridoxine HCl added back in (B6+,~10 ppm) where indicated, Envigo, Madison, WI) and water in standard animal cages under a 12 hr circadian cycle.
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