Leaf thickness variation was measured with a miniature displacement transducer (DF-5.0, Solartron Metrology, Leicester, England; sensor range 5 mm, resolution rated “infinite,” limited only by the controlling hardware). The sensor was placed on the adaxial surface of one young- or middle-aged leaf; the dendrometer was held by a custom-built support (
The dendrometer data were logged and stored every 5 min (hypocotyl: HOBO U12 4-Channel External Data Logger – U12-006, Onset, MA, USA; leaf: CR1000, Campbell Scientific, Logan, UH, USA). The dendrometer data were analyzed using SAS v9.4 for Windows (Statistical Analysis System, SAS Institute, Cary, NC, USA). Hourly measurements were extracted as described by Deslauriers et al. (2011) (link). A smoothing degree of 2 on a scale from 0 to 10 was used to avoid the loss of significant daily variation.