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Guanidine thiocyanate guscn

Manufactured by Merck Group
Sourced in United States

Guanidine thiocyanate (GuSCN) is a chemical compound used in various laboratory applications. It is a chaotropic agent that can effectively disrupt the hydrogen bonds in nucleic acids, proteins, and other biomolecules. GuSCN is commonly used in the extraction and purification of nucleic acids, such as DNA and RNA, from biological samples.

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2 protocols using guanidine thiocyanate guscn

1

Nucleic Acid Extraction and Purification

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Glacial acetic acid, hydrochloric acid, DMSO, titanium (IV) isopropoxide, guanidine HCl (Gu-HCl), and potassium chloride (KCl) were obtained from Fisher Scientific (Waltham, MA, USA). Polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), tetraethyl orthosilicate (TEOS), and guanidine thiocyanate (GuSCN) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Tris base, EDTA, and ethanol were obtained from Acros Organics (part of Thermo Fisher), Promega (Madison, WI, USA), and Decon Labs (King of Prussia, PA, USA), respectively. The Multiscribe Reverse Transcription kit, TaqMan probes, PureLink™ DNA, and miRNA isolation kits were from Life Technologies (Carlsbad, CA, USA). The 5× Taq polymerase and the 25-mM magnesium chloride (MgCl2) solution were from New England Biolabs (Ipswich, MA). All nucleic acids used in the work were purchased from Integrated DNA Technologies, Inc. (IA, USA) with their sequences listed in Table S1 (see Electronic Supplementary Material, ESM).
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2

Synthesis and Characterization of Titanium Dioxide-Graphene Composite Materials

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Titanium tetraisopropoxide (C12H28O4Ti, Sigma-Aldrich), acetic acid (CH3COOH, Sigma-Aldrich), graphite powder (Merck), potassium permanganate (KMnO4, Sigma-Aldrich), phosphoric acid (H3PO4, Sigma-Aldrich), sulfuric acid (H2SO4, Sigma-Aldrich), and hydrogen peroxide (H2O2, Sigma-Aldrich) were used for the synthesis of titanium dioxide, graphene oxide and the composite material. Furthermore, for electron transport electrode fabrication, fluorine-doped tin oxide (FTO)-coated glass was sourced from Sigma-Aldrich and N3 (cis-bis(isothiocyanato)bis(2,2′-bipyridyl-4,4′-dicarboxylato) ruthenium(ii)) dye was acquired from Sigma-Aldrich for the sensitization of the photoanode. A Pt-based electrode was used as a counter electrode of DSSCs.
Iodine (I2, Sigma-Aldrich), guanidine thiocyanate (GuSCN, Sigma-Aldrich), 1,2-dimethyl-3-propylimidazolium iodide (DMPII, Sigma-Aldrich), and 4-tert-butylpyridine (TBP, Sigma-Aldrich) were used for the preparation of the electrolyte that was used in the fabrication of devices. Last, lithium iodide (LiI, Sigma-Aldrich), lithium perchlorate (LiClO4, Sigma-Aldrich) and acetonitrile (CH3CN, Sigma-Aldrich) were used for electrochemical testing of the photoanode.
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