Membrane protein was solubilised in 1× NuPAGE® LDS sample buffer (0.025 mol/L Tris pH 8.5, 0.5% LDS, 2.5% glycerol, 0.05 mol/L dithiothreitol (DTT), 0.05 mol/L SERVA Blue G250, 0.04 mmol/L phenol red). Fifty μg of protein from each sheep was loaded in duplicate lanes on Bolt 4–12% Bis-Tris gels. Proteins were separated in a NuPAGE® MOPS running buffer system (Invitrogen, CA, USA) at a constant 200 V using a mini gel tank (Life technologies, CA, USA). Gels were stained with 0.1% coomassie brilliant blue (CBB R250) in 50% methanol (Chem–supply, SA, Australia) and 10% glacial acetic acid (Chem–supply, SA, Australia), then destained in 40% methanol and 10% glacial acetic acid. Gel images were obtained using a gel doc system (BioRad, CA, USA). Each gel lane was then cut into 9 pieces prior to protein digestion with trypsin and elution from gel piece.
Methanol
Manufactured by Chem-Supply
Sourced in Australia
Methanol is a colorless, flammable liquid chemical compound with the formula CH3OH. It is commonly used as a solvent, fuel, and raw material in the production of other chemicals.
Automatically generated - may contain errors
Lab products found in correlation
Caffeic acid, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Chem-Supply (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Acetonitrile, by Chem-Supply (2 mentions)
Dimethyl sulfoxide (dmso), by Chem-Supply (2 mentions)
Dichloromethane dcm, by Chem-Supply (2 mentions)
Mini gel tank, by Thermo Fisher Scientific (1 mentions)
Gel doc system, by Bio-Rad (1 mentions)
Glacial acetic acid, by Chem-Supply (1 mentions)
Lc ms grade formic acid, by Merck Group (1 mentions)
Porcine pepsin, by Merck Group (1 mentions)
Yeast extract, by Merck Group (1 mentions)
Mucin, by Merck Group (1 mentions)
Bile salt, by Merck Group (1 mentions)
Pectin, by Merck Group (1 mentions)
Pancreatin, by Thermo Fisher Scientific (1 mentions)
Casein, by Merck Group (1 mentions)
Pyrogallol, by Merck Group (1 mentions)
Mrs agar, by Merck Group (1 mentions)
4 methylvaleric acid, by Merck Group (1 mentions)
13 protocols using methanol
1
Membrane Protein Solubilization and Separation
2
Yoghurt Starter Culture and Probiotics
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A commercial yoghurt starter culture (YOFLEX®—consisting of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) and three probiotic strains (Lacticaseibacillus rhamnosus LGG, Lacticaseibacillus casei 431, Bifidobacterium animalis subsp. lactis BB-12) were kindly supplied by Chr. Hansen, Bayswater, VIC, Australia. Powdered skim milk (Australian instant skim milk powder, Coles) was obtained from a local supermarket in Melbourne, VIC, Australia. Pancreatin was purchased from Alfa Aesar (Ward Hill, MA, USA). Mangiferin, pyrogallol, gallic acid, caffeic acid, 3-hydroxy phenyl acetic acid, 3-(2-hydroxyphenyl) propionic acid, porcine pepsin, α-amylase from aspergillus oryzae, p-nitrophenyl-α-d-glucopyranoside, bile salts, LC-MS grade formic acid, acetic acid, 4-methyl valeric acid, guar, casein, tryptone, cysteine HCl, peptone, mucin, pectin, potato starch, yeast extract, and MRS agar were acquired from Sigma–Aldrich (Castle Hill, NSW, Australia). Sodium carbonate (anhydrous), HCl, NaHCO3, NaOH, (NH4)2CO3, KCl, NaCl, KH2PO4, MgCl2(H2O)6, CaCl2, K2HPO4, Na3PO4, MgSO4.7H2O Tween 80, orthophosphoric acid, acetonitrile, and methanol of HPLC grade were acquired from Chem-supply Pty Ltd. (Melbourne, VIC, Australia).
3
Synthesis and Characterization of Platinum(II) Complexes
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All reagents were used as received unless otherwise specified, all organic solvents utilised were of analytical grade, and MilliQTM water (Merck, Melbourne, Australia) was used. Potassium tetrachloroplatinate (K2PtCl4) was acquired from Precious Metals Online. 1S,2S-Diaminocyclohexane (SSDACH, 98%), 1,10-phenanthroline (Phen), 5,6-dimethyl-1,10-phenanthroline (56Me2Phen), silver nitrate, N,N′-dicyclohexylcarbodiimide (DCC), N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC-HCl), N-hydroxysuccinimide (NHS), indomethacin and aspirin were purchased from Sigma-Aldrich, Sydney, NSW. Sep-Pak® C18 columns were obtained from Waters, Australia. Methanol, diethyl ether, ethyl acetate, dichloromethane (DCM), dimethyl formamide (DMF), dimethyl sulfoxide (DMSO) and acetonitrile (ACN) were acquired from Chem-Supply, Adelaide, Australia. Hydrogen peroxide (H2O2) (30%) was purchased from VWR chemicals, Philadelphia, United States. Potassium iodide (KI) was obtained from Merck, Melbourne, Australia. Ultra-pure HNO3 (69%) was acquired from Choice Analytical, Sydney, Australia. The certified reference standard that was used for the ICP-MS experiments was purchased from High-Purity Standards, North Charleston, United States. The deuterated solvents deuterium oxide (D2O, 99.9%) and d6-dimethyl sulfoxide (DMSO-d6, 99.9%) were obtained from Novachem, Melbourne, Australia.
4
Methanol-Carbonate Solution Preparation
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Methanol and sodium carbonate were obtained from Chem Supply Australia; all other reagents were obtained from Sigma Aldrich Australia. All reagents were of analytical grade or higher. Prepared solutions were stored in darkness in a refrigerator (4°C).
5
Synthesis and Characterization of Kiteplatin
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[Pt(1,4-dach)Cl 2 ] (Kiteplatin) was synthesised as per reported previously. 22 All purchased reagents were used as received and all solvents used were of analytical grade or higher. Silver chloride was obtained from BDH chemicals. Dimethyl formamide (DMF) was obtained from Merck. Phen, 5Mephen, 56Me 2 phen, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride and CT-DNA were obtained from Sigma-Aldrich. Methanol was obtained from Chem Supply. Deuterium oxide was obtained from Cambridge Isotope Laboratories.
6
Immunohistochemical Analysis of GFAP in Intestinal Tissues
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Sections of jejuna and colon were cut from paraffin blocks at 4 μm thickness and mounted onto silane-coated slides (Lomb Scientific Pty Ltd). Tissue samples were dewaxed in xylene (Merck Pty Ltd) and rehydrated through a graded series of ethanol (Chem Supply). Sections were immersed in 10 mM citrate buffer (pH 6.0, Sigma-Aldrich Pty Ltd) and antigen retrieval performed by heating sections in a microwave (LG) on high (800 W) until boiling and then on low (160 W) for 10 min. Sections were allowed to cool and endogenous peroxidise activity subsequently blocked with 3 % H 2 O 2 in methanol (Chem Supply). Non-specific antibody binding was blocked with 20 % normal horse serum (NHS) (Sigma-Aldrich Pty Ltd) in PBS for 30 min at room temperature (RT). Avidin-Biotin Blocking Kit (Vector Laboratories) was used to block endogenous avidin-biotin activity. Sections were incubated overnight with a primary goat polyclonal antibody (diluted in 10 % NHS) directed at GFAP at 1:400 (Santa Cruz Biotechnology, #SC-6171) at 4 °C. Sections were then incubated with an appropriate secondary antibody (20 min at RT), ABC labelling reagent (30 min at RT) and developed with 3,3′-Diaminobenzidine. Sections were counterstained with Lillie-Mayer's hematoxylin (HDS Scientific Supplies Pty Ltd), dehydrated and cleared in xylene (Merck Pty Ltd) before being mounted.
7
Photocatalytic Degradation of Organic Pollutants
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Humic acid was purchased from Fluka AG Cheische Fabrik., Buchs, Switzerland and used as a representative natural organic matter compound. Titanium dioxide P25 with 99.8% purity and composed of 80% anatase and 20% rutile phases was acquired from Evonik. The TiO2 had an average particle size of 30 nm and a specific surface area of 50 m2/g). Bovine Serum Albumin (BSA) of molecular weight 66 kDA was purchased from Sigma Aldrich (St. Louis, MO, USA). Screened methyl orange used as a model dye was purchased from Ajax Chemicals, Australia. Methanol was acquired from Chem-Supply, Australia. Nitric acid was purchased from Merck Pty Limited, Kilsyth, Australia. Acetone (99.9%) was purchased from Sigma-Aldrich, Australia. Sintered glass used as the membrane substrate was acquired from Ningbo Ja-Hely Technology Co., Ltd., Ningbo, China. This was in the form of flat circular discs of 25 mm diameter, 2 mm thickness and G5 porosity grade.
8
Synthesis and Characterization of Lactide-Glycolide Copolymer
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3,6-Dimethyl-1,4-dioxane-2,5-dione (lactide, 95%) and 1,4-dioxane-2,5-dione (glycolide, 97%) were purchased from BLDpharm (BLD Pharmatech Ltd., Shanghai, China). Tin (II) 2-ethylhexanoate (92.5–100%), tartrazine trisodium salt (95%), anhydrous dioxane and CDCl3 were obtained from Sigma-Aldrich, St. Louis, MO, USA. Alendronate sodium trihydrate (ALN·3H2O), fluorescamine (>98%) and EDTA were obtained from Carbosynth Ltd., Berkshire, United Kingdom. Methanol, dichloromethane (DCM), tetrahydrofuran (THF), acetonitrile (ACN) and 1-octanol were purchased from ChemSupply, Gillman, Australia. GPC PEG standards were purchased from PSS Polymer Standards Service GmbH, Mainz, Germany. Natureworks 4043D PLA pellets (Mw(GPC) = 200 kDa) were purchased from Filabot, Barre, VT, USA. All chemicals were used as received unless otherwise specified.
9
Analytical Reagent Preparation for Drug Analysis
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Solid analytical reagent grade (AR) atropine sulfate monohydrate and AR 98% sulfuric acid (H2SO4) were purchased from Sigma-Aldrich (Sydney, NSW, Australia). Cocaine hydrochloride (Cocaine.HCl) was obtained from the National Measurement Institute (North Ryde, NSW, Australia). Solid AR KSCN from BDH (Tingalpa, QLD, Australia) was used as internal standard. The impregnation solvent AR methanol was obtained from Chem-Supply (Gillman, SA, Australia).
Stock solutions were prepared at concentrations of 20% (w/v) atropine in 0.5M H2SO4, 20% (w/v) atropine in methanol, 10% (w/v) KSCN in H2O, 20% (w/v) cocaine in 0.5M H2SO4 and 20% (w/v) cocaine in methanol.
Stock solutions were prepared at concentrations of 20% (w/v) atropine in 0.5M H2SO4, 20% (w/v) atropine in methanol, 10% (w/v) KSCN in H2O, 20% (w/v) cocaine in 0.5M H2SO4 and 20% (w/v) cocaine in methanol.
10
Synthesis of Rhodamine B-Doped Silica Nanoparticles
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Cetyltrimethylammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), L-lysine, phosphate buffer solution (PBS) tablets, sodium chloride (NaCl), sodium acetate and rhodamine B were all purchased from Sigma-Aldrich (Castle Hill, NSW Australia). N-hexane, dimethyl sulfoxide (DMSO), methanol (MeOH), hydrochloric acid (HCl) and acetic acid were sourced from ChemSupply (Gillman, SA Australia).
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