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Legend max mouse il 33 elisa kit

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The LEGEND MAX™ Mouse IL-33 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interleukin-33 (IL-33) levels in cell culture supernatants, serum, plasma, and other biological fluids.

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Lab products found in correlation

Mouse il 5 quantikine elisa kit, by R&D Systems (1 mentions) Amicon ultra centrifugal filter unit, by Merck Group (1 mentions) Radioimmunoprecipitation assay buffer, by Thermo Fisher Scientific (1 mentions) Bradford assay kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Mouse IL-33 (3 citations) ELISA MAX kits (48 citations) IL-33 (78 citations) Mouse ELISA kits (37 citations) ELISAs (15 citations)

2 protocols using legend max mouse il 33 elisa kit

1

Quantifying IL-33 and IL-5 in PDAC

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ELISA was done using cell culture conditioned media from PDAC mouse cell lines and ascites fluid from PDAC tumor bearing mice. Cell culture media was concentrated using Amicon Ultra centrifugal filter units (Millipore, Z717185) before conducting ELISA. IL-33 ELISA was performed using LEGEND MAX™ Mouse IL-33 ELISA Kit (Biolegend) using manufacturer’s standard protocol. IL-5 ELISA was performed using Mouse IL-5 Quantikine ELISA Kit (R&D systems) using manufacturer’s standard protocol.
Alam A., Levanduski E., Denz P., Villavicencio H.S., Bhatta M., Alhorebi L., Zhang Y., Gomez E.C., Morreale B., Senchanthisai S., Li J., Turowski S.G., Sexton S., Sait S.J., Singh P.K., Wang J., Maitra A., Kalinski P., DePinho R.A., Wang H., Liao W., Abrams S.I., Segal B.H, & Dey P. (2022). Fungal mycobiome drives IL-33 secretion and type 2 immunity in pancreatic cancer. Cancer cell, 40(2), 153-167.e11.
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2

Quantifying IL-33 in Organ Lysates

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Approximately 20 mg of tissue from kidney, spleen, lung, and liver was homogenized in radioimmunoprecipitation assay buffer (Thermo Fisher Scientific) and then incubated at 4°C for 2 h. Lysates were cleared by centrifugation, and the protein concentration was determined using a detergent-compatible Bradford assay kit (Thermo Fisher Scientific). The concentration of IL-33 in the lysates was determined using the Legend Max Mouse IL-33 ELISA kit (BioLegend) according to the manufacturer’s instructions.
Tuncel J., Benoist C, & Mathis D. (2019). T cell anergy in perinatal mice is promoted by T reg cells and prevented by IL-33. The Journal of Experimental Medicine, 216(6), 1328-1344.
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