Ki 67 antigen

Manufactured by Agilent Technologies

Sourced in China

The Ki-67 Antigen is a protein that is expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). It is widely used as a marker of cell proliferation and is commonly used in the evaluation of tumor cell growth fraction.

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Lab products found in correlation

Rodent block m, by Biocare Medical (1 mentions) Dako autostainer, by Agilent Technologies (1 mentions) Benchmark xt, by Roche (1 mentions) Nat105, by Abcam (1 mentions) Bx51 microscope, by Olympus (1 mentions) Glial fibrillary acidic protein (gfap), by BD (1 mentions) S 100, by BioGenex (1 mentions) Cdc2 p34, by Santa Cruz Biotechnology (1 mentions) Alkaline phosphatase red detection system, by Agilent Technologies (1 mentions) Cyclin b1, by Santa Cruz Biotechnology (1 mentions) Histone h3k36me3 antibody, by Active Motif (1 mentions)

Spelling variants of this product

Ki-67 (5 citations) Monoclonal mouse anti-human Ki-67 antigen (3 citations) Ki-67 nuclear antigen (3 citations) Mouse anti-human Ki-67 antigen monoclonal antibody (3 citations)

4 protocols using ki 67 antigen

Comprehensive Tumor Histology Analysis

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Tumor histology was evaluated through H&E staining on a Dako autostainer (Agilent). Three micrometer sections from formalin-fixed, paraffin-embedded (FFPE) HT29 tumor were incubated with the appropriate serum designed for blocking endogenous mouse IgG and non-specific background in mouse tissues (Rodent Block M; Biocare Medical), and then incubated overnight at 4ºC using primary antibodies: anti-Ki-67 (1:75, Ki-67 Antigen (Dako Omnis) Clone MIB-1); cleaved caspase-3 antibody (1:250, Monoclonal Rabbit IgG Clone #269518 anti-human cleaved caspase-3 (Asp175) antibody); mouse monoclonal anti-human PD-1 (1:50 [NAT105] Abcam), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (1:400, D13.14.4E, XP Rabbit mAb #4370-CST), phospho-p38 MAPK (Thr180/Tyr182) (1:400, D3F9, XP Rabbit mAb #4511-CST). Anti-PD-1, predilute, NAT105 (Cell marque) in a Benchmark XT (Ventana Medical Systems) was used for section from patients with colon cancer. The IHC staining was evaluated in at least 10 consecutive not overlapping high-power field (HPF) ×400 magnification (0.237 mm²/field) in at least five areas using an Olympus BX51 microscope (Olympus, Tokyo, Japan). Stained sections were independently evaluated by expert pathologist/researchers blinded to initial assessments.

Ieranò C., Righelli D., D'Alterio C., Napolitano M., Portella L., Rea G., Auletta F., Santagata S., Trotta A.M., Guardascione G., Liotti F., Prevete N., Maiolino P., Luciano A., Barbieri A., Di Mauro A., Roma C., Esposito Abate R., Tatangelo F., Pacelli R., Normanno N., Melillo R.M, & Scala S. (2022). In PD-1+ human colon cancer cells NIVOLUMAB promotes survival and could protect tumor cells from conventional therapies. Journal for Immunotherapy of Cancer, 10(3), e004032.

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Immunohistochemical Analysis of Glioma Biomarkers

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Microscopic sections were cut at 4–6 microns for hematoxylin and eosin-stained sections and immunohistochemical (IHC) studies. IHC studies were performed on an automatic Leica immunostainer (Leica Biosystems, Buffalo Grove, IL), and included Ki-67 antigen (Dako, clone MIB-1, 1:100 dilution), GFAP (BD Biosciences, 4A11, clone 1B4, 2E1, 1:7000), S-100 (BioGenex, clone 15E2E2, 1:900), IDH1 R132H mutant protein (Dianova, clone H09, 1:40), p53 (mouse monoclonal, Dako, Cat#M7001, dilution 1:100, clone DO-7), EGFR (mouse monoclonal, Source: Calbiochem, cat#GR01, dilution 1:150, clone 528) and PTEN (mouse monoclonal, Source: Dako, cat#M3627, dilution 1:100, clone 6H2.1). All controls were appropriate.

Cachia D., Kamiya-Matsuoka C., Mandel J.J., Olar A., Cykowski M.D., Armstrong T.S., Fuller G.N., Gilbert M.R, & De Groot J.F. (2015). Primary and secondary gliosarcomas: clinical, molecular and survival characteristics. Journal of neuro-oncology, 125(2), 401-410.

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Immunohistochemical Detection of Cell Cycle Markers

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The alkaline phosphatase/RED detection system (Dako, cat. #: K5005) was used for immunohistochemistry and immunochemistry on formalin-fixed and paraffin-embedded tissue or cell pellets via the avidin-biotin-complex-method. The following antibodies were used: Anti-Histone H3.3 G34W (RevMAb Biosciences, cat. #: 31-1145-00, 1:400), Wee1 (Santa Cruz Biotechnology, cat. #: sc-037, 1:25), Cdc2 p34 (Santa Cruz Biotechnology, cat. #: sc-54, 1:200), Cyclin B1 (Santa Cruz Biotechnology, cat. #: sc-245, 1:25), Histone H3K36me3 Antibody (Active Motif, cat. #: 61021, 1:8000), R2 (Santa Cruz Biotechnology, cat. #: sc-10844, 1:100), and Ki-67 Antigen (Dako, cat. #: M7240, 1:200). As retrieval methodes all sides were pretreated by a steamer in EDTA pH 8. Consensus evaluation was done on multihead microscope (CL, TFB, PM).

Lübbehüsen C., Lüke J., Seeling C., Mellert K., Marienfeld R., von Baer A., Schultheiss M., Möller P, & Barth T.F. (2019). Characterization of Three Novel H3F3A-mutated Giant Cell Tumor Cell Lines and Targeting of Their Wee1 Pathway. Scientific Reports, 9, 6458.

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Immunohistochemical Evaluation of Tumor Proliferation

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Paraffin sections (4-μm thick) were routinely stained with hematoxylin and eosin. To assess tumor cell proliferation, immunohistochemical staining for Ki67 antigen (DakoCytomation, Hangzhou, China) was performed. Briefly, after deparaffinization and rehydration, the sections were immersed in 0.3% hydrogen peroxide to quench intrinsic peroxidase activity. The diluted antibodies were then added to the sections and incubated at 37°C for 1 h. The labeled antigen was visualized with the Histofine kit (Bosde, Wuhan, China), followed by reaction with 3,3′-diaminobenzidine. Finally, the sections were counterstained with hematoxylin. Ki67-positive cells were determined by counting about 500 nuclei in randomly selected microscopic fields, and the Ki67 labeling index was expressed as the ratio of Ki67-positive cells to total cells.

Yin Q., Liu S., Dong A., Mi X., Hao F, & Zhang K. (2016). Targeting Transforming Growth Factor-Beta1 (TGF-β1) Inhibits Tumorigenesis of Anaplastic Thyroid Carcinoma Cells Through ERK1/2-NF-κB-PUMA Signaling. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 2267-2277.

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