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2 protocols using prostaglandin e1 pge1

1

SARS-CoV-2 Spike Protein Interaction Assays

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SARS-CoV-2 (2019-nCoV) spike protein was purchased from Sino Biological. Coenzyme Q10 (CoQ10), 2′,7′-Dichlorofluorescin diacetate (DCFH-DA), ADP, thrombin from human plasma, and N-acetylcysteine (NAC) were obtained from sigma-Aldrich (St. Louis, MO, USA). Collagen was obtained from Chrono-Log Corp. (Havertown, PA, USA). FITC mouse anti-human CD62P and FITC Mouse Anti-Human PAC-1 were purchased from BD bioscience (San Jose, CA, USA). Alexa FluorTM 488-conjugated fibrinogen was purchased from Thermo Fisher Scientific (Waltham, MA, USA). TMRM assay kit (mitochondrial membrane potential) was purchased from Abcam (Cambridge, UK). Prostaglandin E1 (PGE1) was obtained from MedChemExpress (Monmouth Junction, NJ, USA). MDA, SOD, and T-AOC assay kits were purchased from Nanjing Jiancheng (Nanjing, China) and the 8-iso-PGF-2α assay kit was obtained from Andy gene (Beijing, China).
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2

Lipid-Based Nanocarrier Characterization

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1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) and egg yolk lecithin (EPC, PL-100M) were purchased from AVT Pharmaceutical Tech Co., Ltd. (China). Prostaglandin E1 (PGE1) was purchased from MedChemExpress (USA). Protease inhibitor mini-tablets, Dulbecco's modified Eagle medium (DMEM), Roswell Park Memorial Institute (RPMI) 1640 medium, and penicillin–streptomycin (10,000 U/mL) were purchased from Thermo Fisher (USA). CD41/CD61 antibody (362802) was from BioLegend (USA). GPVI antibody (STJ192229) was from StJohnsLabs (UK). CD29 antibody (UUY0319031), CD49b antibody (CBDM011909A), and CD42b antibody (CANBO41909A) were from BD Biosciences (USA). CD62P antibody (AB6631) and CD63 antibody (ab134045) were from Abcam (UK). Fluorescence dyes 3,3′-dioctadecy-loxacarbocyanine perchlorate salt (DiO), 1,1′-dioctadecyl-3,3,3′,3′-tetra-methylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD), 4ʹ,6-diamidino-2-phenylindole (DAPI) were purchased from Meilunbio (China), and CFSE was from eBioscience (USA). Coumarin 6 and Rhodamine B were purchased from Sigma–Aldrich (USA). Rhodamine-conjugated dextran was purchased from Shanxi Xinyan Bomei Biotechnology Co., Ltd. (China). Omni-Easy™ Instant BCA protein assay kit was from EpiZyme (USA). EDTA, cholesterol, chloroform, and ethanol were purchased from Sinopharm (China).
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