UV–visible spectra were recorded using an Agilent Technologies Evolution 300 spectrophotometer. The fluorescence experiments were performed on a Horiba Fluorolog-3 spectrometer. Fluorescence decay profiles were recorded on a FLS920 instrument. The experimental quantum yields were determined by recording the emission signals within an integrating light sphere on a FLS980 Photoluminescence Spectrometer (Edinburgh Instruments) equipped with an ozone-free Xenon Arc Lamp (450 W), photomultiplier R928P and double grating excitation and emission monochromators (Czerny-Turner type).
Fls980 photoluminescence spectrometer
Manufactured by Edinburgh Instruments
Sourced in United Kingdom
The FLS980 Photoluminescence Spectrometer is a laboratory instrument designed for the measurement of photoluminescence (PL) spectra. It features a high-performance monochromator, sensitive detectors, and comprehensive software control for accurate and reliable PL data acquisition and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Xe1 xenon arc lamp, by Edinburgh Instruments (2 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Fluorolog 3 spectrometer, by Horiba (1 mentions)
Eos 550, by Canon (1 mentions)
F 4600 spectrophotometer, by Hitachi (1 mentions)
Tecnai f20 microscope, by Thermo Fisher Scientific (1 mentions)
Ihr550 spectrometer, by Horiba (1 mentions)
7 protocols using fls980 photoluminescence spectrometer
1
UV-Vis and Fluorescence Spectroscopy
2
Gentamicin Accumulation Assay in A. baumannii
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The gentamicin accumulation assay was performed using the method described previously (43 (link)). Briefly, A. baumannii AB5075_UW wild-type and its dksA::Tn26 mutant were grown to OD = 0.6 in MH broth. Culture aliquots (500 μl) were transferred to 2 ml sterile Eppendorf tubes, and gentamicin-Texas Red conjugate was added to each sample at a final gentamicin concentration of 500 μg/ml. Reactions were protected from light and incubated for 30 min at 37°C with shaking at 200 rpm. Cells were then pelleted by centrifugation at 8000 g for 1 min, washed with 400 μl PBS, and the pellet resuspended in 1 ml DMSO and stored at −20°C prior to measurement.
Photophysical measurements were performed with a FLS980 photoluminescence spectrometer (Edinburgh Instruments) equipped with a Xe1 Xenon Arc Lamp (450 W ozone free, excitation range 230–1000 nm) for steady-state measurements. Excitation (lex) was performed at 550 nm, and emission spectra were recorded in DMSO at 28°C with 1 nm step-size, 0.1s integration time, and slit-width of Δlex = Δlem = 1.5 nm for both strains.
Photophysical measurements were performed with a FLS980 photoluminescence spectrometer (Edinburgh Instruments) equipped with a Xe1 Xenon Arc Lamp (450 W ozone free, excitation range 230–1000 nm) for steady-state measurements. Excitation (lex) was performed at 550 nm, and emission spectra were recorded in DMSO at 28°C with 1 nm step-size, 0.1s integration time, and slit-width of Δlex = Δlem = 1.5 nm for both strains.
3
Gentamicin Uptake Assay in Acinetobacter
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Gentamicin uptake assays were performed as follows: A. baumannii ATCC17978 was grown to OD = 0.6 in Mueller-Hinton II medium. 500 µl culture aliquots were transferred to 2 ml sterile Eppendorf tubes, with or without BAC added at 4 µg.ml−1, and gentamicin-TR was added to each reaction at a final gentamicin concentration of 1 µg.ml−1. Reactions were protected from light and incubated for 30 minutes at 37 °C 200 RPM. Cells were then pelleted by centrifugation at 8000 × g for 1 minute, washed with 400 µl PBS, and the pellet resuspended in 1 ml DMSO and stored at -20 °C prior to measurement. Gentamicin uptake assays were performed in the same way with A. baumannii AB5075, except that cells were incubated with 4 µg.ml−1 BAC and 500 µg.ml−1 gentamicin-TR.
Photophysical measurements were performed with a FLS980 photoluminescence spectrometer (Edinburgh Instruments) equipped with a Xe1 Xenon Arc Lamp (450 W ozone free, excitation range 230 nm – 1000 nm) for steady-state measurements. Excitation (λex) was performed at 550 nm and emission spectra were recorded in DMSO at 28 °C with 1 nm step-size, 0.1s integration time, and slit-width of ∆λex = ∆λem = 1.5 nm for AB5075 or ∆λex = ∆λem = 5 nm for ATCC17978.
Photophysical measurements were performed with a FLS980 photoluminescence spectrometer (Edinburgh Instruments) equipped with a Xe1 Xenon Arc Lamp (450 W ozone free, excitation range 230 nm – 1000 nm) for steady-state measurements. Excitation (λex) was performed at 550 nm and emission spectra were recorded in DMSO at 28 °C with 1 nm step-size, 0.1s integration time, and slit-width of ∆λex = ∆λem = 1.5 nm for AB5075 or ∆λex = ∆λem = 5 nm for ATCC17978.
4
Characterizing Photophysical Properties of Metal-Organic Frameworks
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Photoluminescence spectra were recorded on an Edinburgh FLS980 Photoluminescence Spectrometer. Before the fluorescence emission test, the equivalent solid-state Pd-PCN-222(Hf) and Pt@Pd-PCN-222(Hf) samples were finely ground to avoid error. The photoluminescence lifetime experiments were performed by the time-correlated single photo counting (TCSPC) method by using a 406.5 nm picosecond pulsed diode laser, or by using the same spectrometer but with a μF900 Xe lamp.
For ultrafast transient absorption (TA) measurements, the samples were dispersed in CH3CN in a 0.7 mL sealed quartz cuvette, and then measurements were carried out using a femtosecond pump-probe setup consisting of Helios (Ultrafast Systems LLC) spectrometers and a regeneratively amplified Ti:sapphire laser source (Coherent Legend, 800 nm, 150 fs, 5 mJ per pulse, and 1 kHz repetition rate) in the transmission mode. A pump light of 400 nm was generated by the frequency doubling portion of the 800 nm output (75%) pulse in a BaB2O4 (BBO) crystal, and the power intensity can be adjusted by a range of neutral-density filters, which were fixed at 125 μJ cm–2 in this experiment. The delay time window between the pump and probe pulse from 0 to 7.7 ns was controlled by a motorized delay time stage.
For ultrafast transient absorption (TA) measurements, the samples were dispersed in CH3CN in a 0.7 mL sealed quartz cuvette, and then measurements were carried out using a femtosecond pump-probe setup consisting of Helios (Ultrafast Systems LLC) spectrometers and a regeneratively amplified Ti:sapphire laser source (Coherent Legend, 800 nm, 150 fs, 5 mJ per pulse, and 1 kHz repetition rate) in the transmission mode. A pump light of 400 nm was generated by the frequency doubling portion of the 800 nm output (75%) pulse in a BaB2O4 (BBO) crystal, and the power intensity can be adjusted by a range of neutral-density filters, which were fixed at 125 μJ cm–2 in this experiment. The delay time window between the pump and probe pulse from 0 to 7.7 ns was controlled by a motorized delay time stage.
5
Comprehensive Characterization of Luminescent Materials
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Transmission electron microscopy (TEM) observations were performed on a Tecnai F20 microscope (FEI, Hillsboro, OR, USA). X-ray photoelectron spectroscopy (XPS) was carried out with ESCALAB 250Xi (Thermo Scientific, Waltham, MA, USA). Fourier transform infrared (FT-IR) spectra were obtained on a Nicolet 6700 FT-IR spectrometer (Thermo Nicolet Corp., Madison, WI, USA). Photoluminescence, phosphorescence emission and excitation spectra were measured on a Hitachi F-4600 spectrophotometer (Hitachi, Tokyo, Japan) at ambient conditions. UV-Vis absorption spectra were recorded on a PERSEE T10CS UV-Vis spectrophotometer (Persee, Beijing, China). FL and Phos lifetimes were measured using an EDINBURGH FLS 980photoluminescence spectrometer (Edinburgh Instruments, Wales, UK). Photographs of FL and Phos images were taken using a Canon camera (EOS 550, Tokyo, Japan) under a hand-held blue LED lamp (420 nm). PL QY measurements were carried out with a QE-2100 quantum efficiency measurement system (Otsuka Electronics, Tokyo, Japan).All the FL and RTP spectra and lifetimes were measured under aerobic atmosphere in this study unless otherwise noted.
6
Variable-excitation Energy PL Measurements
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Variable-excitation
energy PL
measurements were acquired with an Edinburgh Instruments FLS980 photoluminescence
spectrometer at wavelengths of 250 and 275 nm. Confocal PL data was
acquired with a Horiba-Jobin-Yvon iHR550 spectrometer with an excitation
wavelength of 405 nm.
energy PL
measurements were acquired with an Edinburgh Instruments FLS980 photoluminescence
spectrometer at wavelengths of 250 and 275 nm. Confocal PL data was
acquired with a Horiba-Jobin-Yvon iHR550 spectrometer with an excitation
wavelength of 405 nm.
7
Photoluminescence of InP/ZnS Quantum Dots
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Steady-state photoluminescence measurements on InP/ZnS QDs in the range 500–800 nm (excitation wavelength λex = 480 nm) were acquired using the FLS-980 Photoluminescence spectrometer (Edinburgh Instruments Ltd., Livingston, UK).
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