Dfc 295 camera

Manufactured by Nikon

The Nikon DFC 295 is a digital camera designed for laboratory and scientific applications. It captures high-quality images with a resolution of 2.9 megapixels. The camera features a C-mount lens adapter, allowing it to be used with a variety of microscope and optical systems. The DFC 295 supports various image formats and provides connectivity options for data transfer and integration with scientific workflows.

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Lab products found in correlation

Tms f light microscope, by Nikon (1 mentions) μ slide 8 well, by Ibidi (1 mentions) Cm1860, by Leica (1 mentions) Eclipse e200 microscope, by Nikon (1 mentions)

2 protocols using dfc 295 camera

Quantifying Spheroid Size and Volume

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After the last 4^th passage single cells were plated at a density of 3 × 10⁴ cells/ml or after irradiation at a density of 5 × 10⁴ cells/ml in an uncoated μ-Slide 8 well (iBidi, GmbH, Martinsried, Germany). Representative pictures for each n were taken in 4 consistent regions of each well using a Leica DFC 295 camera and a Nikon TMS-F light microscope. For the irradiation experiment a Nikon ECLIPSE TE20 microscope with an INFINITY1–2C camera and Infinite analyze software were used to capture the pictures.
The diameter of each sphere was measured and the corresponding volume (in μm³) of the sphere was calculated offline for the 24 and 48 hour time using Image J 1.45s (Wayne Rasband, NIH, USA).

Zanni G., Di Martino E., Omelyanenko A., Andäng M., Delle U., Elmroth K, & Blomgren K. (2015). Lithium increases proliferation of hippocampal neural stem/progenitor cells and rescues irradiation-induced cell cycle arrest in vitro. Oncotarget, 6(35), 37083-37097.

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Cardiac Tissue Histological Analysis

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Hearts were thawed, dissected, submerged in embedding medium (O.C.T. Compound, 23-730-57; Thermo Fisher Scientific), and flash-frozen in isopentane at −140°C for 30 s and stored at −80°C until sectioned in a cryostat (model CM1860; Leica). Samples were sectioned at 10 µm thickness, placed on slides, and stored at −20°C until stained. Slides were fixed with 4% paraformaldehyde, stained with hematoxylin and eosin as previously described (Fenwick et al., 2019 (link)), and covered with glass coverslips. Images were acquired using LAS X imagine software, Leica DFC295 camera, and Nikon Eclipse E200 microscope at 0.4 numerical aperture and 200× total magnification. Image processing was performed using ImageJ.

Turner K.L., Morris H.S., Awinda P.O., Fitzsimons D.P, & Tanner B.C. (2023). RLC phosphorylation amplifies Ca2+ sensitivity of force in myocardium from cMyBP-C knockout mice. The Journal of General Physiology, 155(4), e202213250.

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