Pgl3 promoter reporter plasmids

Manufactured by Promega

Sourced in United States

PGL3-promoter reporter plasmids are laboratory tools that contain a luciferase reporter gene under the control of a specific promoter sequence. These plasmids are used to study the activity and regulation of the promoter of interest in various cell lines and experimental conditions.

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Lab products found in correlation

Pcdna3, by Thermo Fisher Scientific (1 mentions) Tianamp genomic dna kit, by Tiangen Biotech (1 mentions) Kod plus mutagenesis kit, by Toyobo (1 mentions)

Close spelling variants of this product

PGL3-promoter plasmid PGL3 reporter plasmid PGL3-promoter PGL3 plasmid Reporter plasmids

2 protocols using pgl3 promoter reporter plasmids

Exploring ALYREF and MYC regulation

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The ALYREF promoter and MYC 3ʹ-UTR sequences were inserted into the pGL3-promoter reporter plasmids (Promega, USA). GBM cells were transfected with ALYREF siRNA, GFP, MYC overexpression plasmids and reporter plasmids and were lysed with PLB buffer. Luciferase values were measured using BioTek (USA).

Wang J., Li Y., Xu B., Dong J., Zhao H., Zhao D, & Wu Y. (2021). ALYREF Drives Cancer Cell Proliferation Through an ALYREF-MYC Positive Feedback Loop in Glioblastoma. OncoTargets and therapy, 14, 145-155.

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Cloning and Mutating Mouse Htra1 Enhancers

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Genomic DNA was extracted from wild-type mouse livers using a TIANamp Genomic DNA Kit (TIANGEN, Beijing, China). Enhancer fragments, including Htra1-E0 (mm9, chr7: 138079707-138079807), Htra1-E1 (mm9, chr7: 138081131-138081254), Htra1-E2 (mm9, chr7: 138086759-138086863), Htra1-E3 (mm9, chr7: 138089914-138090014), Htra1-E4 (mm9, chr7: 138093068-138093168), Htra1-E5 (mm9, chr7: 138105019-138105146), Htra1-E6 (mm9, chr7: 138108952-138109052), Htra1-E7 (mm9, chr7: 138109623-138109723), Htra1-E8 (mm9, chr7: 138111903-138112003), and Htra1-E9 (mm9, chr7: 138112161-138112270) were cloned from genomic DNA and inserted into pGL3-promoter reporter plasmids (Promega, Madison, WI). The mouse Egr1 sequence was cloned from cDNA templates and inserted into pcDNA3.1(−) (Invitrogen). The primers sequences are shown in Table S1. The deletion mutations of RUNX2-binding sites on Htra1 enhancers were performed using a KOD-Plus Mutagenesis Kit (Toyobo, Osaka, Japan), following the manufacturer’s instructions. Mutant vectors carrying the RUNX2- or EGR1-binding sites were defined as MRUNX2-E0 to MRUNX2-E9 and MEGR1-E0 to MEGR1-E9, respectively. The primers sequences for mutation are shown in Table S3.

Zhang Q., Zuo H., Yu S., Lin Y., Chen S., Liu H, & Chen Z. (2020). RUNX2 co-operates with EGR1 to regulate osteogenic differentiation through Htra1 enhancers. Journal of cellular physiology, 235(11), 8601-8612.

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