The developing tongue was isolated from E15.5 mouse and cultured on 1.0 μm Nucleapore Track-Etch Membrane (Whatman, United States) in medium at 37°C and 5% CO2 for 72 h using a slight modification of the culture method reported by Trowell (Kim et al., 2009 (link)). The culture medium (DMEM/F12, Invitrogen, United States) was supplemented with 2 mM GlutaMAX (Invitrogen, United States), 10 mM HEPES buffer (Sigma-Aldrich, United States), 2% B27 (Invitrogen, United States) and 1% penicillin/streptomycin and was renewed every 24 h.
Nucleapore track etch membrane
Manufactured by Cytiva
Sourced in United States
The Nucleapore Track-Etch Membrane is a laboratory filtration product. It is a thin, porous membrane made from polycarbonate material. The membrane contains a high density of uniform, precisely sized pores that are created through a controlled track-etching process.
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Lab products found in correlation
2 protocols using nucleapore track etch membrane
1
Culturing Developing Mouse Tongue
2
Embryonic Mouse Tongue Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The tongue was isolated from E12.5 mouse embryos and cultured on a 1.0 μm Nucleapore Track-Etch Membrane (Whatman, Pittsburg, PA, United States) in the medium at 37°C and 5%CO2 for 48 h using culture method reported by Trowell. The culture medium (DMEM, Invitrogen, United States) was supplemented with 10% fetal bovine serum (FBS) (Invitrogen, United States) and 1% penicillin/streptomycin and was renewed every 24 h.
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