First, conventional flexible bronchoscopy (BF-260 Bronchovideoscope; Olympus; Tokyo, Japan) was used for observation, using a siliconized, uncuffed tracheal tube with an inside diameter of 7.5 mm (Portex; Smiths Medical, St. Paul, MN, USA). Then, EBUS-TBNA was performed using a convex probe EBUS bronchoscope (BF-UC260F-OL8, Olympus; Tokyo, Japan). In pretreatment, 25 mg hydroxyzine pamoate were used by intramuscular injection. 5 ml of 2% lidocaine was sprayed into the pharynx and 5 ml of 2% lidocaine was administered through the channel during the procedures. The bronchoscope was inserted orally during midazolam induced conscious sedation. Patients were monitored by electrocardiogram, pulse oximetry and blood pressure without the presence of an anesthesiologist. The examination of the enlarged mediastinal lymph node stations accessible by EBUS (stations 2, 4 and 7) as well as the hilar lymph nodes (stations 10 and 11) was performed.
Bf uc260f ol8
Manufactured by Olympus
Sourced in Japan
The BF-UC260F-OL8 is a high-performance laboratory microscope designed for a variety of applications. It features a binocular observation tube, a UIS2 optical system, and a focus adjustment knob. The microscope is equipped with a built-in LED illumination system. Technical specifications and detailed information about the intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
Na 201sx 4022, by Olympus (10 mentions)
Portex, by Smith & Nephew (3 mentions)
Bf 260 bronchovideoscope, by Olympus (2 mentions)
Eu c2000, by Olympus (2 mentions)
Bf uc260fw, by Olympus (2 mentions)
Model hdi 5000, by Philips (1 mentions)
Echo hd 22 ebus o, by Cook Medical (1 mentions)
Bact alert mp, by bioMérieux (1 mentions)
Na 201sx 4021, by Olympus (1 mentions)
Eb 530us, by Fujifilm (1 mentions)
Na 2015x 4022, by Olympus (1 mentions)
Eu c60, by Olympus (1 mentions)
18 protocols using bf uc260f ol8
1
Mediastinal Lymph Node Examination via EBUS-TBNA
2
EBUS-TBNA Procedure for Lymph Node Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EBUS‐TBNA procedures were performed by trained operators using a convex probe‐EBUS bronchoscope (BF‐UC260F‐OL8; Olympus) and a dedicated 22‐gauge needle (NA‐201SX‐4022; Olympus). The patients were under moderate sedation, achieved with intravenous midazolam and fentanyl.12 , 17 Lidocaine was used for local anesthesia. Hilar, interlobar, and lobar LNs recognized by real‐time ultrasound were examined by the operator. When possible, we conducted three passes per node. When core tissue was obtained, at least two passes were conducted when possible. However, when we obtained enough core tissue at the first pass and the patient's condition was unstable, we prematurely terminated the procedure after the first pass.
Once the tissue core had been secured, it was blotted in filter paper to remove excess blood, fixed in formalin, and then the tissue coagulum clot was sent for histological examination.18 Aspirate specimens were expelled onto glass slides, smeared, immediately fixed, and sent for cytological and/or histological examination.17 Rapid on‐site cytopathological evaluation was not performed.
Information on EBUS‐TBNA‐related adverse events (e.g., bleeding, hypoxemia, etc) was also collected.
Once the tissue core had been secured, it was blotted in filter paper to remove excess blood, fixed in formalin, and then the tissue coagulum clot was sent for histological examination.
Information on EBUS‐TBNA‐related adverse events (e.g., bleeding, hypoxemia, etc) was also collected.
3
Transbronchial Lymph Node Biopsy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Patients had no contraindication for bronchoscopy, the puncture was performed in the bronchoscopy examination room of the outpatient department. The patients fasted before the operation. A total of 2% lidocaine was used to achieve anaesthesia by thyrocricoid puncture, and 2 mg of midazolam was used intravenously for sedation. First, routine bronchoscopy was performed and then BF-UC260F-OL8 (Olympus Ltd. Tokyo) was used to enter the airway through the mouth to the check and the target lymph nodes and surrounding blood vessels. The diameter of the target lymph nodes was recorded by the ultrasonic image processing device, a 22G special puncture needle was used to enter the lesion with the penetration method, and the negative pressure syringe was connected to the end of the biopsy needle. Each lymph node was punctured 3 times; if the lump was near the trachea, it could also be punctured, and the method is described above.
4
EBUS-TBNA for Lymph Node Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EBUS-TBNA was performed using a dedicated bronchoscope with a linear ultrasound transducer (BF-UC260F-OL8; Olympus, Tokyo, Japan). The target LN were punctured with a 21-gauge needle (NA-201SX-4022; Olympus, Tokyo, Japan). All patients provided written informed consent before undergoing the bronchoscopy. Two or more punctures were performed on each target lymph node to obtain at least two tissue core specimens. One of those specimens was prepared for histological examination, while the other specimens were utilized for AFB, Xpert, and MGIT960 culture. Ultrasonography was conducted with model HDI 5000, 7–12 MHz (Philips Medical Systems, Bothell, WA, USA) to evaluate the sizes (in cm) of superficial LN by measuring the largest diameters. Nodal masses with size > 5 mm were identified as bulky lesions. The size of LN was assessed by measuring the largest and smallest diameters on the ultrasound screen, and the long axis/short axis (L/S) ratio was calculated.
5
EBUS-TBNA for Biopsy of Lung Masses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Food and water were forbidden for at least 6 hours before EBUS-TBNA. EBUS-TBNA was performed using an ultrasound system and a linear ultrasonic bronchoscope (BF-UC260F-OL8, Olympus Ltd, Tokyo, Japan). All patients received local anesthesia with lidocaine. EBUS-TBNA was performed by senior doctors in all cases. White light bronchoscopy was performed prior to examination of the target mass and peripheral vessels with EBUS. A 22-gauge needle was used to biopsy the target mass under real-time ultrasound guidance. Two or three aspirations were typically required in order to obtain enough histology specimens. Cytology and histology were performed by two independent pathologists. No onsite cytologic evaluation was performed.18 (link),19 (link)
6
EBUS-TBNA for Lung Cancer Molecular Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EBUS-TBNA was performed using an ultrasound bronchoscope and a dedicated ultrasound processor (convex probeEBUS, BF-UC260F-OL8 and EU-C2000, Olympus, Tokyo, Japan). Needle aspiration was performed with a 22-gauge needle (NA-201SX-4022, Olympus). The procedure was performed with the patient under conscious sedation (midazolam) and local anesthesia (lidocaine). One or more target lesions were selected at the bronchoscopists’ discretion. When the main lesion that was suspicious for recurrence or progression was not accessible by EBUS-TBNA, the bronchoscopists selected targets that were still suspicious for malignancy and accessible by EBUS-TBNA as the second option. Thansbronchial needle aspirations were performed on target lesions. We allowed additional aspirations by endoscopic ultrasound with bronchoscope-guided fine needle aspiration (EUS-B-FNA) when we encountered difficulties with EBUS-TBNA. The aspirate was placed into a solution of 10% neutral-buffered formalin. Cell-blocks were prepared and stained with hematoxylin and eosin. EGFR mutation analysis was performed with direct sequencing as described in the literature [20 (link)] or peptide nucleic acid–mediated polymerase chain reaction clamping. A pathologist (G.K.L.) performed the cytopathologic and molecular examinations.
7
EBUS-TBNA Procedure for Mediastinal Lymph Node Sampling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Before EBUS-TBNA, the target lymph nodes for sampling were selected according to enlarged mediastinal or hilar lymph node with a short axis > 10 mm in thorax CT or maximum standardized uptake (SUVmax) value > 2.5 in PET-CT. The lymph node map was determined according to the classification proposed by the International Association for the Study of Lung Cancer[15 (link)].
EBUS-TBNA was performed in an outpatient setting using a flexible bronchoscope (BF-UC260F-OL8; Olympus, Tokyo, Japan) by 1 of 2 experienced endoscopists (QW or SJL). First, local anesthesia was applied via aerosol inhalation and intratracheal spray of 2% lidocaine. Then, the EBUS scope was introduced, and all reachable lymph node stations were examined. Sampling was performed from mediastinal and hilar lymph nodes that had been previously identified as suspicious by imaging and were able to be accessed by EBUS-TBNA. For each target lesion, real-time punctures were made using a standard 22-gauge needle (ECHO-HD-22-EBUS-O; Cook Medical, Bloomington, IN, United States). Attempts were made to acquire both cytological and histological specimens during sampling, if possible (Figure1 ). Major complications (e.g., serious hemorrhage > 100 mL, pneumothorax, and post-procedure infection) that occurred during and/or after surgery were recorded.
EBUS-TBNA was performed in an outpatient setting using a flexible bronchoscope (BF-UC260F-OL8; Olympus, Tokyo, Japan) by 1 of 2 experienced endoscopists (QW or SJL). First, local anesthesia was applied via aerosol inhalation and intratracheal spray of 2% lidocaine. Then, the EBUS scope was introduced, and all reachable lymph node stations were examined. Sampling was performed from mediastinal and hilar lymph nodes that had been previously identified as suspicious by imaging and were able to be accessed by EBUS-TBNA. For each target lesion, real-time punctures were made using a standard 22-gauge needle (ECHO-HD-22-EBUS-O; Cook Medical, Bloomington, IN, United States). Attempts were made to acquire both cytological and histological specimens during sampling, if possible (Figure
8
EBUS-TBNA for Mediastinal Lymph Node Sampling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All bronchoscopic procedures were performed under conscious sedation with local anesthesia by three pulmonary physicians (Eom JS, Mok JH, and Lee K). Before EBUS-TBNA, conventional bronchoscopy was conducted in a standard fashion for airway inspection and administration of lidocaine into the tracheobronchial tree via the working channel of the bronchoscope. Following conventional bronchoscopy, EBUS-TBNA was performed using a dedicated bronchoscope with a linear ultrasound transducer (BF-UC260F-OL8; Olympus, Tokyo, Japan). Systemic assessment of the mediastinal, hilar, and interlobar lymph nodes was made based on computed tomography (CT) findings [12 (link)], and target lymph nodes were punctured with a 22-gauge needle (NA-201SX-4022; Olympus, Tokyo, Japan) under real-time ultrasound guidance. Two or more punctures were performed on each target lymph node until at least two tissue core specimens were obtained. One of the tissue core specimens was placed in formalin for histological examination. Other tissue core specimens that were placed in sterile saline were analyzed by fluorescence microscopy using auramine-rhodamine staining. Additionally, solid (3 % Ogawa medium) and liquid media (BacT/ALERT MP; bioMérieux, Durham, NC, USA) were used to culture for mycobacteria from the specimens in sterile saline.
9
EBUS-TBNA for Mediastinal Lymph Node Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All bronchoscopic procedures were performed on an in-patient basis. Conventional flexible bronchoscopy (BF-260 Bronchovideoscope, Olympus; Tokyo, Japan) was performed for observational purposes, using a siliconized, uncuffed tracheal tube with an inner diameter of 7.5 mm (Portex; Smiths Medical, St. Paul, MN, USA). EBUS-TBNA was then performed using a convex-probe EBUS bronchoscope (BF-UC260F-OL8, Olympus; Tokyo, Japan). Prior to the latter procedure, the patients were administered 25 mg of hydroxyzine pamoate by intramuscular injection. Of note, 5 ml of 2% lidocaine was first sprayed into the pharynx and then administered again through the working channel during the examination. The bronchoscope was inserted orally in patients under conscious sedation induced by midazolam or a combination of midazolam and fentanyl. Patients were monitored by electrocardiogram, pulse oximetry and blood pressure without the presence of an anesthesiologist. Mediastinal lymph nodes accessible by EBUS (stations 2, 4 and 7) and hilar lymph nodes (stations 10 and 11) were examined to identify those with a SUVmax >2.5.
10
Endoscopic Ultrasound-Guided Lymph Node Biopsy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In the respiratory endoscopy center, patients signed the informed consent, and then underwent analgesia for the performance of EBUS-TBNA. CP-EBUS was only used with Endoscopic system: Olympus BF-UC260F-OL8, BF-UC260FW (Olympus Corporation, Japan), puncture needle, Olympus (21G, NA-201SX-4022, NA-201SX-4021)(Olympus Corporation, Japan). EBUS-TBNA were performed by experienced doctors, mediastinal and hilar lymph nodes, as well as centrally located parenchymal lesions and visible with endobronchial ultrasound were punctured by needles for 3–5 times, the sample obtained using EBUS -TBNA needle were tissue or cells by aspiration, which were fixed with formalin and sent to the pathology department for histopathological examinations.
The final diagnosis final diagnosis were confirmed by surgical biopsy and or by long-term follow-up and evaluation of treatment effects. In this study, all of the the patients were followed-up for 1 year.
The final diagnosis final diagnosis were confirmed by surgical biopsy and or by long-term follow-up and evaluation of treatment effects. In this study, all of the the patients were followed-up for 1 year.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!