RNA was isolated from NHEKs using Purelink RNA isolation columns (ThermoFisher Scientific) according to manufacturer’s instructions. RNA was quantified using a Nanodrop spectrophotometer (ThermoFisher Scientific), and 500ng of RNA was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad, Irvine, CA). qPCR reactions were ran on a CFX96 Real-Time Detection System (Bio-Rad) using gene-specific primers and TaqMan probes (ThermoFisher Scientific).
Purelink rna isolation columns
Manufactured by Thermo Fisher Scientific
The Purelink RNA isolation columns are designed for the rapid and efficient extraction of total RNA from a variety of biological samples. The columns utilize a silica-based membrane to selectively bind RNA, allowing for the removal of contaminants and the subsequent elution of high-quality RNA.
Automatically generated - may contain errors
2 protocols using purelink rna isolation columns
1
RNA Extraction and qPCR Analysis in NHEKs
2
NHEK RNA Isolation and qPCR Protocol
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RNA was isolated from NHEKs using Purelink RNA isolation columns (ThermoFisher Scientific) according to the manufacturer’s instructions. RNA was quantified using a Nanodrop spectrophotometer (ThermoFisher Scientific), and up to 500 ng of RNA was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad, Irvine, CA). Quantitative real-time PCR reactions were run on a CFX96 real-time detection system (Bio-Rad) using gene-specific primers and TaqMan probes (ThermoFisher Scientific). PCR primers and probes are shown on Table S2 .
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