Leucine

Manufactured by Thermo Fisher Scientific

Sourced in United States

Leucine is an essential amino acid that plays a crucial role in protein synthesis and various metabolic processes. It is a colorless, crystalline solid that is commonly used in laboratory settings for analytical and research purposes.

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Lab products found in correlation

7 protocols using leucine

Remdesivir Dry Powder Inhaler Formulation

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The Remdesivir for formulation was purchased from Medkoo Biosciences (Research Triangle Park, NC, USA). Remdesivir, GS-441524, and its heavy isotope internal standards were purchased from Alsachim (Illkirch-Graffenstaden, France). Lactose monohydrate, leucine, polysorbate 20, acetonitrile (HPLC grade), and trifluoracetic acid were purchased from Fisher Scientific (Pittsburgh, PA, USA). D-Mannitol was bought from Acros Organic (Fair lawn, NJ, USA). Dipalmitoylphosphotidylcholine (DPPC) was purchased from Avanti Polar Lipid, Inc. (Alabaster, AL, USA). Cholesterol, albumin, transferrin, ascorbic acid and Hanks’ Balanced Salt solution (HBBS) were purchased from Sigma-Aldrich (St.Louis, MO, USA). Sulfobutylether-beta-cyclodextrin (SBECD, Captisol^®) was kindly provided by CyDex Pharmaceuticals, Inc. (Lawrence, KS, USA) High-resistance Monodose RS00 dry powder inhalers were kindly provided by Plastiape S.p.A. (Osnago, Italy).

Sahakijpijarn S., Moon C., Koleng J.J., Christensen D.J, & Williams RO I.I.I. (2020). Development of Remdesivir as a Dry Powder for Inhalation by Thin Film Freezing. Pharmaceutics, 12(11), 1002.

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Remdesivir Sample Preparation Protocol

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Remdesivir for sample preparation was purchased from Medkoo Biosciences (Research Triangle Park, NC, USA). Remdesivir, GS-441524, and its heavy isotope internal standards were purchased from Alsachim (Illkirch-Graffenstaden, France). Leucine was purchased from Fisher Scientific (Pittsburgh, PA, USA). Sulfobutylether-beta-cyclodextrin (SBECD, Captisol®) was kindly provided by Ligand Pharmaceuticals, Inc. (San Diego, CA).

Sahakijpijarn S., Moon C., Warnken Z.N., Maier E.Y., DeVore J.E., Christensen D.J., Koleng J.J, & Williams RO I.I.I. (2021). In vivo pharmacokinetic study of remdesivir dry powder for inhalation in hamsters. International Journal of Pharmaceutics: X, 3, 100073.

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Potassium Ferrate(VI) Preparation and Characterization

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Carbamazepine (CBZ, >98% purity),
sulfadimethoxine (SDM, >98% purity), trimethoprim (TMP, >98%
purity),
all test amino acids (i.e., glycine, alanine, serine, leucine, proline,
phenylalanine, glutamic acid, asparagine, and histidine), phenyl methyl
sulfoxide (PMSO), phenyl methyl sulfone (PMSO₂), sodium
thiosulfate, and sodium borate were purchased from Fisher Scientific
(Austin, Texas, USA). A wet chemical procedure was applied to prepare
solid potassium ferrate(VI) (K₂FeO₄),^{59 (link)} which had a purity of more than 90%. The solid
K₂FeO₄ was dissolved in a 0.01 M sodium borate
buffer solution. The concentration of Fe(VI) solution was determined
by measuring the absorbance at 510 nm using a 1.0 cm path length and
a molar absorption coefficient of ε_510nm = 1150 M^–1 cm^–1,^{59 (link)} with a UV–visible spectrometer (Thermo Scientific Co., USA).
All reaction solutions were prepared using Milli-Q ultrapure water
(>18 MΩ cm^–1 resistivity, Millipore, Milford,
USA).

Sharma V.K., Wang J., Feng M, & Huang C.H. (2023). Oxidation of Pharmaceuticals by Ferrate(VI)–Amino Acid Systems: Enhancement by Proline. The Journal of Physical Chemistry. a, 127(10), 2314-2321.

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Yeast Cell Culture Media Compositions

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Standard culture media were used [50 ]. The YPD medium consists of 1% yeast extract (Becton, Dickson, and Co., Sparks, MD), 2% Peptone (Becton, Dickson, and Co.), and 2% dextrose (Fisher Scientific, Fair Lawn, NJ). The synthetic complete (SC) medium consists of 0.67% yeast nitrogen base without amino acids (Becton, Dickson, and Co.), 0.0086% of each of the 20 amino acids except 0.0171% leucine (Fisher Scientific), 0.0021% adenine (Fisher Scientific), 0.0009% 4-aminobenzoic acid (Fisher Scientific), 0.0086% uracil (Sigma, St. Louis, MO), 0.0086% myo-inositol (Sigma), and 2% dextrose (Fisher Scientific). The SC-Dropout medium is the same as SC, except that specific amino acid(s) were omitted. The YM-1, a buffered rich liquid medium [51 (link)], consists of 0.5% yeast extract (Becton, Dickson, and Co.), 1% Peptone (Becton, Dickson, and Co.), 0.7% yeast nitrogen base without amino acids (Becton, Dickson, and Co.), 0.6% sodium hydroxide (Fisher Scientific), 1% succinic acid (Fisher Scientific), and 2% dextrose (Fisher Scientific). All yeast strains were grown at 25°C, unless otherwise indicated.

Oh Y., Schreiter J.H., Okada H., Wloka C., Okada S., Yan D., Duan X, & Bi E. (2017). Hof1 and Chs4 interact via F-BAR domain and Sel1-like repeats to control extracellular matrix deposition during cytokinesis. Current biology : CB, 27(18), 2878-2886.e5.

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Niclosamide Formulation Development

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Niclosamide was purchased from Shenzhen Nexconn Pharmatechs LTD. (Shenzhen, China). Tert-butanol (TBA), acetone, acetonitrile, leucine, polysorbate 20, trifluoracetic acid were acquired from Fisher Scientific (Pittsburgh, PA, USA). Pearlitol® PF-mannitol was purchased from Roquette America (Keokuk, IA, USA).

Jara M.O., Warnken Z.N., Sahakijpijarn S., Moon C., Maier E.Y., Christensen D.J., Koleng J.J., Peters J.I., Hackman Maier S.D, & Williams III R.O. (2021). Niclosamide inhalation powder made by thin-film freezing: Multi-dose tolerability and exposure in rats and pharmacokinetics in hamsters. International Journal of Pharmaceutics, 603, 120701.

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Defined Minimal Medium for Yeast

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Defined minimal medium contained 6.7 g/L of yeast nitrogen base without amino acids (catalog no. Y0626; Sigma-Aldrich, MO, USA), 20 g/L of glucose, 380 mg/L leucine (catalog no. 172130250; Acros Organics, CA, USA), 76 mg/L uracil (catalog no. 157301000; Acros Organics, CA, USA), and various concentrations of [EMIM][OAc] (>95% purity) (IoLiTec, AL, USA). Synthetic complete medium without leucine and uracil (SC-Leu-Ura) was prepared with 6.7 g/L of yeast nitrogen base without amino acids; 1.46 g/L of yeast synthetic dropout medium supplement without uracil, leucine, and tryptophan (catalog no. Y1771; Sigma-Aldrich, MO, USA); 76 mg/L tryptophan (catalog no. 172110250; Acros Organics, CA, USA), 20 g/L glucose, and various concentrations of [EMIM][OAc]. SC without leucine (SC-Leu) was prepared by adding 76 mg/L uracil to SC-Leu-Ura medium.

Walker C., Ryu S., Garcia S., Dooley D., Mendoza B, & Trinh C.T. (2022). Gene Coexpression Connectivity Predicts Gene Targets Underlying High Ionic-Liquid Tolerance in Yarrowia lipolytica. mSystems, 7(4), e00348-22.

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Defined Minimal Media for Yeast Culture

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Growth medium. Defined minimal media contained 6.7 g/L of yeast nitrogen base without amino acids (cat# Y0626, Sigma-Aldrich, MO, USA), 20 g/L of glucose, 380 mg/L leucine (cat# 172130250, Acros Organics, CA, USA), 76 mg/L uracil (cat# 157301000, Acros Organics, CA,

Walker C., Ryu S., García S., Dooley D., Mendoza B., & Trinh C.T. (2022). Gene Co-expression Connectivity Predicts Gene Targets Underlying High Ionic Liquid Tolerance in Yarrowia lipolytica.

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