Nrf2 sirna

Manufactured by Santa Cruz Biotechnology

Sourced in United States, China

Nrf2 siRNA is a laboratory tool designed to selectively target and reduce the expression of the Nrf2 (Nuclear factor erythroid 2-related factor 2) gene. Nrf2 is a transcription factor that plays a crucial role in the regulation of cellular responses to oxidative stress. The Nrf2 siRNA product provides researchers with a reliable means to investigate the function and signaling pathways associated with Nrf2 in various experimental models.

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Lab products found in correlation

Control sirna, by Santa Cruz Biotechnology (11 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (8 mentions) Scrambled sirna, by Santa Cruz Biotechnology (6 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (5 mentions) Silentfect lipid reagent, by Bio-Rad (3 mentions) Sirna transfection reagent, by Santa Cruz Biotechnology (2 mentions) Rnaimax, by Thermo Fisher Scientific (2 mentions) Sirnas, by Qiagen (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (2 mentions) Control sirna, by Qiagen (2 mentions) Scrambled control sirna, by Santa Cruz Biotechnology (2 mentions) Lipofectamine 2000 sirna transfection reagent, by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000, by Addgene (2 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Sirna transfection medium, by Santa Cruz Biotechnology (2 mentions) Ho 1 sirna, by Santa Cruz Biotechnology (2 mentions) Dharmafect 4 transfection reagent, by GE Healthcare (1 mentions) Rpmi 1640 cell culture medium, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

SiRNAs (95 citations) Nrf2 specific siRNA (4 citations) Nrf2 small interfering RNA (siRNA) (4 citations) Control siRNA and Nrf2 siRNA (2 citations)

54 protocols using nrf2 sirna

Silencing Grx1, Grx2 and Nrf2 in Human RPE

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Scramble, Grx1, Grx2 and Nrf2 siRNA used in this study were chemically synthesized by Santa Cruz Biotechnology (Santa Cruz, CA, USA). SiRNA duplexes (scrambled siRNA, sc-37007; Grx1 siRNA, sc-72089; Grx2 siRNA, sc-72090; Nrf2 siRNA, sc-37030) were transfected into primary human RPE cell with siRNA transfection reagent (sc-29528; Santa Cruz, CA, USA) according to the manufacturer’s instructions.

Liu X., Xavier C., Jann J, & Wu H. (2016). Salvianolic Acid B (Sal B) Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Cell Death by Activating Glutaredoxin 1 (Grx1). International Journal of Molecular Sciences, 17(11), 1835.

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Nrf2 Silencing in Human RPE Cells

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Control and Nrf2 siRNA used in this study were chemically synthesized by Santa Cruz Biotechnology (Santa Cruz, CA, USA). SiRNA duplexes (scrambled siRNA, sc-37007; Nrf2 siRNA, sc-72089) were transfected into primary human RPE cell with siRNA transfection reagent (sc-29528; Santa Cruz, CA, USA) according to the manufacturer’s instructions.

Liu X., Ward K., Xavier C., Jann J., Clark A.F., Pang I.H, & Wu H. (2015). The novel triterpenoid RTA 408 protects human retinal pigment epithelial cells against H2O2-induced cell injury via NF-E2-related factor 2 (Nrf2) activation. Redox Biology, 8, 98-109.

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Nrf2 Knockdown and Lipoxin A4 Effects

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IEC-6 cells were maintained in Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin antibiotics (5% CO₂) at 37°C. Commercial Nrf2 siRNA (Santa Cruz Biotechnology) was used for inhibition of Nrf2 expression according to the manufacturer's protocol. After transfection with nonsilencing control siRNA and Nrf2 siRNA, cells were incubated in Dulbecco's Modified Eagle's Medium for 48 hours. Each experiment was performed at least in triplicate. IEC-6 cells were collected and mRNA expression of Nrf2 level was detected by real time Polymerase Chain Reaction using the following primers: 5′-GGTGATGAATTTTACTCTGC-3′ (sense) and 5′-TTTCCGAGTCACTGATGAACC-3′ (anti-sense) for Nrf2 and 5′-GTCGGTGTGAACGGATTT-3′ (sense) and 5′-ACTCCACGACGTACTCAGC-3′ (anti-sense) for GAPDH. Before H/R injury, the cells in some of the subgroups were exposed to Lipoxin A4 (10 nM) for 12 hours [27 (link)].

Han X., Yao W., Liu Z., Li H., Zhang Z.J., Hei Z, & Xia Z. (2016). Lipoxin A4 Preconditioning Attenuates Intestinal Ischemia Reperfusion Injury through Keap1/Nrf2 Pathway in a Lipoxin A4 Receptor Independent Manner. Oxidative Medicine and Cellular Longevity, 2016, 9303606.

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Nrf2 siRNA Transfection in HUVECs

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HUVECs were cultured in medium containing 20 µg of Nrf2 siRNA or scrambled siRNA
(Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 6 hours per day on 2
consecutive days according to the manufacturer’s instructions.

Wang D., Hou J., Wan J., Yang Y., Liu S., Li X., Li W., Dai X., Zhou P., Liu W, & Wang P. (2021). Dietary chlorogenic acid ameliorates oxidative stress and improves endothelial function in diabetic mice via Nrf2 activation. The Journal of International Medical Research, 49(1), 0300060520985363.

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Nrf2 Silencing in bEnd.3 Cells

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bEnd.3 cells were seeded at 30,000 cells/well and transfected with 40 pmol/well of either scrambled siRNA or Nrf2 siRNA (Santa Cruz, USA) [28 (link)] for 24 h using Dharmafect 4 transfection reagent (GE Healthcare, USA), as previously described [29 (link)].

Warpsinski G., Smith M.J., Srivastava S., Keeley T.P., Siow R.C., Fraser P.A, & Mann G.E. (2020). Nrf2-regulated redox signaling in brain endothelial cells adapted to physiological oxygen levels: Consequences for sulforaphane mediated protection against hypoxia-reoxygenation. Redox Biology, 37, 101708.

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Nrf2 and NF-κB Signaling Pathway Analysis

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Unless otherwise noted, analytical chemicals were from Sigma (St. Louis, MO). Antibodies to Nrf2, NF-κB p65, and lamin B1, and β-tubulin were from Cell Signaling Technology, Inc. (Danvers, MA). TriZol reagent, NE-PER Nuclear and Cytoplasmic Extraction Reagents, Reverse Transcription kit, SYBR Green PCR master mixture, and RPMI 1640 cell culture medium were from Thermal Fisher Scientific Inc. (Thermal Fisher, Rockford, IL). NF-κB SN50 inhibitor was from EMD Millipore (Billerica, MA). Nrf2 siRNA, negative control siRNA and Nrf2 primers were from Santa Cruz Biotechnology (Dallas, TX). Nucleofactor^™ Kits for THP-1 was purchased from (Lonza, Rockland, ME).

Zhang H., Zhou L., Yuen J., Birkner N., Leppert V., O’Day P.A, & Forman H.J. (2017). Delayed Nrf2-regulated antioxidant gene induction in response to silica nanoparticles. Free radical biology & medicine, 108, 311-319.

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Investigating Nrf2 in α-Synuclein Overexpression

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PC12 cells transfected with A53T α-syn genes were stimulated with Dox to achieve the overexpression of α-syn (inducible PC12/α-syn cells), and then these cells were seeded in 6-well plates at a density of 2 × 10⁵ cells/well and allowed to adhere for 12 h. Cells were transfected with Nrf2 siRNA or scrambled siRNA (Santa Cruz, CA, USA) for 48 h using SureFECT transfection reagent according to the manufacturer’s instructions. After induction of Dox (1 µg/mL) for 24 h to induce the expression of α-syn, cells were treated with SDA for another 24 h and then collected for Western blot assay.

Sheng X., Yang S., Wen X., Zhang X., Ye Y., Zhao P., Zang L., Peng K., Du E, & Li S. (2021). Neuroprotective effects of Shende’an tablet in the Parkinson’s disease model. Chinese Medicine, 16, 18.

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Silencing of ATF4 and Nrf2 in HT22 Cells

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For siRNA transfection, HT22 cells were plated in 60 mm dishes at 5 × 10⁵ cells/dish and 20 pmol ATF4 siRNA (#sc-35113), Nrf2 siRNA (#sc-37049) or control siRNA (#sc-37007), all from Santa Cruz Biotechnology were used along with RNAi max (Invitrogen) according to the manufacturer's instructions.

Fischer W., Currais A., Liang Z., Pinto A, & Maher P. (2018). Old age-associated phenotypic screening for Alzheimer's disease drug candidates identifies sterubin as a potent neuroprotective compound from Yerba santa. Redox Biology, 21, 101089.

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Nrf2 Silencing in Mesencephalic Cells

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Nrf2 siRNA (sc-37049, Santa Cruz, CA, USA) and control siRNA (sc-37007, Santa Cruz, CA, USA) were dissolved separately in Optimem I (Invitrogen, CA, USA). After 10 min of equilibration at room temperature, each RNA solution was combined with the respective volume of the Lipofectamine 2000 solution (Invitrogen, CA, USA), mixed gently and allowed to form siRNA liposomes for 20 min. The primary cultured mesencephalic cells were transfected with the transfection mixture in antibiotic-free cell culture medium for 72 h before MPP⁺ or LPS treatment, and subjected to various measurements.

Zhou J., Qu X.D., Li Z.Y., W.e.i.-.J.i., Liu Q., Ma Y.H, & He J.J. (2014). Salvianolic Acid B Attenuates Toxin-Induced Neuronal Damage via Nrf2-Dependent Glial Cells-Mediated Protective Activity in Parkinson’s Disease Models. PLoS ONE, 9(7), e101668.

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Nrf2 Regulation by Farrerol

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Cells were inoculated into a 6-well plate and grown until the number of cells reached approximately 50% confluence. Cells were then transfected with the control siRNA or Nrf2 siRNA (Santa Cruz, CA, USA) using Lipofectamine 2000 (Thermo Fisher, Madison, USA) according to the manufacturer's instructions. After transfection, the cells were incubated with farrerol for 24 h. The protective effect of farrerol was evaluated using western blot analysis.

Ma N., Yang X., Qi C., Yu Q., Zhu C, & Ren H. (2021). Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK. Oxidative Medicine and Cellular Longevity, 2021, 8847844.

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