Mouse anti α sma cy3 conjugate

Immunohistochemistry was performed as described previously[21 (link)] on 10 μm frozen sections of cold phosphate-buffered saline perfused brains taken from mice subject to normoxia (control) or hypoxia for 4, 7, and 14 days. Each slide contained mouse brains representing the four different time-points of hypoxia, to ensure consistent antibody incubation times across different time-points. The following rat monoclonal antibodies were obtained from BD Pharmingen (La Jolla, CA, USA): anti-CD31 (clone MEC13.3), anti-β4 integrin (clone 346-11A), and anti-CD151 (clone 455807). Other primary antibodies used included mouse anti-α-SMA-Cy3 conjugate (Sigma, clone 1A4), hamster anti-CD31 (clone 2H8, Abcam, Cambridge, MA, USA), rabbit anti-CD151 (Creative Diagnostics, Shirley, NY, USA), and guinea pig polyclonal anti-plectin (Progen, Heidelberg, Germany). Secondary antibodies used included anti-rat-Cy3, anti-rabbit-Cy3, and anti-guinea pig-Cy3 (Jackson ImmunoResearch, West Grove, PA, USA), anti-Armenian Hamster-Dy-Light 594 (BioLegend, San Diego, CA, USA), and anti-rat Alexa Fluor 488 and anti-guinea pig Alexa Fluor 488 (Invitrogen Corporation, Carlsbad, CA, USA).