Allophycocyanin conjugated rat anti mouse cd25

Humanized mAbs (5A12) against CD147 were generated and identified in our laboratory. A purified human IgG1 isotype control antibody (Clone: ET901) was purchased from BioLegend, Inc. (San Diego, CA, USA). An anti-mouse CD147 functional-grade purified antibody (Clone: RL73) and its isotype control (Clone: eBR2a) were purchased from eBioscience (San Diego, CA, USA). Tacrolimus (FK506) was purchased from KeHao (Wuhan, Hubei, China). Fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD4 and phycoerythrin (PE)-conjugated mouse anti-human CD3 antibodies, Foxp3 staining buffer and a cell activation cocktail used for intracellular staining were purchased from BioLegend. PE-Cy7-conjugated rat anti-mouse IFN-γ, PE-conjugated rat anti-mouse IL-4, Alexa 647-conjugated rat anti-mouse IL-17, FITC-conjugated rat anti-mouse CD4, allophycocynanin-H7-conjugated rat anti-mouse CD8, PE-Cy7-conjugated rat anti-mouse CD44, BUV737-conjugated rat anti-mouse CD62 L, allophycocyanin-conjugated rat anti-mouse CD25, and PE-conjugated rat anti-mouse Foxp3 antibodies were purchased from BD Biosciences (San Diego, CA, USA).