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Prcytomag 40k

Manufactured by Merck Group
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PRCYTOMAG-40K is a magnetic bead-based cytometry reagent kit designed for the quantification of protein and cell surface marker expression. The kit provides a standardized platform for high-throughput analysis of up to 40 parameters simultaneously using flow cytometry technology.

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Lab products found in correlation

Procyte dx hematology analyzer, by IDEXX (2 mentions) Hcmp2mag 19k, by Merck Group (2 mentions) Bio plex 200 system, by Bio-Rad (1 mentions)

7 protocols using prcytomag 40k

1

Non-Human Primate Cytokine Profiling

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In non-human primates blood was collected at 0 and 15 minutes and 6, 12, 24, 28, 48 and 72 hours after infusion. Serum was analyzed with an Olymus AU400 chemistry analyzer. Whole blood samples were also analyzed with an IDEXX procyte DX hematology analyzer. Levels of IL-6, TNFɑ and CCL2 were measured using Milliplex detection kits HCMP2MAG-19k and PRCYTOMAG-40k (Millipore Corporation, St Charles, MO, USA). Samples were run in duplicate.
Lameijer M., Binderup T., van Leent M.M., Senders M.L., Fay F., Malkus J., Sanchez-Gaytan B.L., Teunissen A.J., Karakatsanis N., Robson P., Zhou X., Ye Y., Wojtkiewicz G., Tang J., Seijkens T.T., Kroon J., Stroes E.S., Kjaer A., Ochando J., Reiner T., Pérez-Medina C., Calcagno C., Fischer E.A., Zhang B., Temel R.E., Swirski F.K., Nahrendorf M., Fayad Z.A., Lutgens E., Mulder W.J, & Duivenvoorden R. (2018). Efficacy and safety assessment of a TRAF6-targeted nanoimmunotherapy in atherosclerotic mice and non-human primates. Nature biomedical engineering, 2(5), 279-292.
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2

Non-Human Primate Cytokine Profiling

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In non-human primates blood was collected at 0 and 15 minutes and 6, 12, 24, 28, 48 and 72 hours after infusion. Serum was analyzed with an Olymus AU400 chemistry analyzer. Whole blood samples were also analyzed with an IDEXX procyte DX hematology analyzer. Levels of IL-6, TNFɑ and CCL2 were measured using Milliplex detection kits HCMP2MAG-19k and PRCYTOMAG-40k (Millipore Corporation, St Charles, MO, USA). Samples were run in duplicate.
Lameijer M., Binderup T., van Leent M.M., Senders M.L., Fay F., Malkus J., Sanchez-Gaytan B.L., Teunissen A.J., Karakatsanis N., Robson P., Zhou X., Ye Y., Wojtkiewicz G., Tang J., Seijkens T.T., Kroon J., Stroes E.S., Kjaer A., Ochando J., Reiner T., Pérez-Medina C., Calcagno C., Fischer E.A., Zhang B., Temel R.E., Swirski F.K., Nahrendorf M., Fayad Z.A., Lutgens E., Mulder W.J, & Duivenvoorden R. (2018). Efficacy and safety assessment of a TRAF6-targeted nanoimmunotherapy in atherosclerotic mice and non-human primates. Nature biomedical engineering, 2(5), 279-292.
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3

Multiplexed Serum Cytokine Profiling

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Serum samples were stored at -80°C prior to analysis. Samples were tested by the Cytokine Reference Laboratory (University of Minnesota) using the magnetic bead set PRCYTOMAG-40K (EMD Millipore). Samples were analyzed for Non-Human Primate (NHP)-specific TNFα, IFNγ, IL-6 & IL-2 using the Luminex platform and performed as a multi-plex. Fluorescent color-coded beads coated with a specific capture antibody were added to each sample. After incubation and washing, biotinylated detection antibody was added followed by phycoerythrin-conjugated streptavidin. The beads were read on a Luminex instrument (Bioplex 200). Samples were run in duplicate and values were interpolated from five-parameter fitted standard curves.
Pampusch M.S., Abdelaal H.M., Cartwright E.K., Molden J.S., Davey B.C., Sauve J.D., Sevcik E.N., Rendahl A.K., Rakasz E.G., Connick E., Berger E.A, & Skinner P.J. (2022). CAR/CXCR5-T cell immunotherapy is safe and potentially efficacious in promoting sustained remission of SIV infection. PLoS Pathogens, 18(2), e1009831.
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4

Multiplex Cytokine Profiling in NHP

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Serum samples were stored at -80°C prior to analysis. Samples were tested by the Cytokine Reference Laboratory (University of Minnesota) using the magnetic bead set PRCYTOMAG-40K (EMD Millipore). Samples were analyzed for Non-Human Primate (NHP)-specific TNFα, IFNγ, IL-6, IL-1β and IL-2 using the Luminex platform and performed as a multi-plex. Fluorescent color-coded beads coated with a specific capture antibody were added to each sample. After incubation and washing, biotinylated detection antibody was added followed by phycoerythrin-conjugated streptavidin. The beads were read on a Luminex instrument (Bioplex 200). Samples were run in duplicate and values were interpolated from five-parameter fitted standard curves.
Pampusch M.S., Sevcik E.N., Quinn Z.E., Davey B.C., Berg J.M., Gorrell-Brown I., Abdelaal H.M., Rakasz E.G., Rendahl A, & Skinner P.J. (2023). Assessment of anti-CD20 antibody pre-treatment for augmentation of CAR-T cell therapy in SIV-infected rhesus macaques. Frontiers in Immunology, 14, 1101446.
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5

Cytokine Profile in SIV-Infected Macaques

Cited 1 time
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Concentrations of multiple cytokines and chemokines (GM-CSF, TNF-β, G-CSF, IFN-γ, IL-2, IL-10, IL-15, sCD40L, IL-17a, IL-1Ra, IL-13, IL-1β, IL-4, IL-5, IL-6, IL-8, MIP-1α, MCP-1, TNF-α, MIP-1β, vascular endothelial growth factor [VEGF], IL-18, and IL-12) were measured in the CSF of the infected animals within a few days of each PET scan and just before necropsy, using a bead-based multiplex assay (EMD Millipore; PRcytomag-40K). These were chosen based on literature support for cytokine abnormalities in the setting of HIV/SIV infection and the availability of antibodies that are specific to macaques (14 (link)– (link)16 (link)). The assay was performed according to the manufacturer’s instructions. The assay plates were read on the Bio-Plex 200 System (Bio-Rad).
Hammoud D.A., Sinharay S., Shah S., Schreiber-Stainthorp W., Maric D., Muthusamy S., Lee D.E., Lee C.A., Basuli F., Reid W.C., Wakim P., Matsuda K., Hirsch V., Nath A, & Di Mascio M. (2019). Neuroinflammatory Changes in Relation to Cerebrospinal Fluid Viral Load in Simian Immunodeficiency Virus Encephalitis. mBio, 10(3), e00970-19.
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6

Cytokine Profiling in Transplant Patients

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Serum IL-1β, TNF-α, IL-6, IL-17, IL-12 (P40) and IL-10 levels at 1 week (CIBMT; n=7, CKBMT; n=3) and 4 weeks (CIBMT; n=8, CKBMT; n=7) after transplantation were determined by Luminex (Life Technologies, Carlsbad, CA) using Non-Human Primate Cytokine, PRCYTOMAG-40K (Merck Millipore, Billerica, MA), following the manufacturer's instructions.
Oura T., Ko D.S., Boskovic S., O'Neil J.J., Chipashvili V., Koulmanda M., Hotta K., Kawai K., Nadazdin O., Smith R.N., Cosimi A.B, & Kawai T. (2015). Kidney versus Islet Allograft Survival after Induction of Mixed Chimerism with Combined Donor Bone Marrow Transplantation. Cell transplantation, 25(7), 1331-1341.
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7

Multiplex Serum Cytokine Profiling

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Serum samples were stored at -80°C prior to analysis. Samples were tested by the Cytokine Reference Laboratory (University of Minnesota) using the magnetic bead set PRCYTOMAG-40K (EMD Millipore). Samples were analyzed for Non-Human Primate (NHP)-specific TNF, IFN, IL-6 & IL-2 using the Luminex platform and performed as a multi-plex. Fluorescent colorcoded beads coated with a specific capture antibody were added to each sample. After incubation and washing, biotinylated detection antibody was added followed by phycoerythrin-conjugated streptavidin. The beads were read on a Luminex instrument (Bioplex 200). Samples were run in duplicate and values were interpolated from five-parameter fitted standard curves.
Pampusch M.S., Abdelaal H.M., Cartwright E.K., Molden J., Davey B.C., Sauve J.D., Rendahl A., Rakasz E.G., Connick E., Berger E.A., & Skinner P.J. (2021). CAR/CXCR5-T cell immunotherapy is safe and potentially efficacious in promoting sustained remission of SIV infection.
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