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Cd45 percp cy5.5 clone hi30

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CD45-PerCp-Cy5.5 (clone HI30) is a fluorescent-labeled monoclonal antibody used for the identification and enumeration of cells expressing the CD45 antigen. CD45 is a pan-leukocyte marker expressed on the surface of all hematopoietic cells. The PerCp-Cy5.5 fluorochrome is attached to the anti-CD45 antibody, allowing for the detection and quantification of CD45-positive cells in flow cytometry applications.

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Lab products found in correlation

Tim3 bv510 clone f38 2e2, by Thermo Fisher Scientific (2 mentions) Cd45ro pe cy7 clone uchl1, by BD (2 mentions) Cd45ra fitc or bv711 clone hi100, by BD (2 mentions) Ccr7 bv421 clone 150503, by BD (2 mentions) Cd122 bv510 clone mik β3, by BD (2 mentions) Cd4 buv395 clone sk3, by BD (2 mentions) Cd8 buv805 clone sk1, by BD (2 mentions) Cd22 fc and her2 fc recombinant proteins, by R&D Systems (2 mentions) Dylight488 and or 650 antibody labeling kits, by Thermo Fisher Scientific (2 mentions) Cd3 pacblue clone hit3a, by Thermo Fisher Scientific (2 mentions) Cd62l bv605 clone dreg 56, by BD (2 mentions)

Close spelling variants of this product

PerCP-Cy5.5 (120 citations) CD45-PerCP (82 citations) CD45-PerCP-Cy5.5 (51 citations) CD45 (clone HI30; (10 citations) PerCP-Cy5.5 CD45 (9 citations) CD45 (HI30, (6 citations) Clone HI30 (5 citations)

2 protocols using cd45 percp cy5.5 clone hi30

1

Comprehensive Immunophenotyping of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-CD19 CAR idiotype antibody was kindly provided by Bipulendu Jena and Laurence Cooper48 . The 1A7 anti-14G2a idiotype antibody was obtained from NCI-Frederick. CD22 and Her2 CARs were detected using human CD22-Fc and Her2-Fc recombinant proteins (R&D). The idiotype antibodies and Fc-fusion proteins were conjugated in house with Dylight488 and/or 650 antibody labeling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
From BioLegend: CD4-APC-Cy7 (clone OKT4), CD8-PerCp-Cy5.5 (clone SK1), TIM3-BV510 (clone F38–2E2), CD39-FITC or APC-Cy7 (clone A1), CD95-PE (clone DX2), CD3-PacBlue (clone HIT3a),
From eBioscience: PD1-PE-Cy7 (clone eBio J105), LAG3-PE (clone 3DS223H), CD45RO-PE-Cy7 (clone UCHL1), CD45-PerCp-Cy5.5 (clone HI30),
From BD: CD45RA-FITC or BV711 (clone HI100), CCR7-BV421 (clone 150503), CD122-BV510 (clone Mik-β3), CD62L-BV605 (clone DREG-56), CD4-BUV395 (clone SK3), CD8-BUV805 (clone SK1).
Lynn R.C., Weber E.W., Sotillo E., Gennert D., Xu P., Good Z., Anbunathan H., Lattin J., Jones R., Tieu V., Nagaraja S., Granja J., DeBourcy C., Majzner R., Satpathy A.T., Quake S.R., Monje M., Chang H, & Mackall C.L. (2019). c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature, 576(7786), 293-300.
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2

Comprehensive Immunophenotyping of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-CD19 CAR idiotype antibody was kindly provided by Bipulendu Jena and Laurence Cooper48 . The 1A7 anti-14G2a idiotype antibody was obtained from NCI-Frederick. CD22 and Her2 CARs were detected using human CD22-Fc and Her2-Fc recombinant proteins (R&D). The idiotype antibodies and Fc-fusion proteins were conjugated in house with Dylight488 and/or 650 antibody labeling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
From BioLegend: CD4-APC-Cy7 (clone OKT4), CD8-PerCp-Cy5.5 (clone SK1), TIM3-BV510 (clone F38–2E2), CD39-FITC or APC-Cy7 (clone A1), CD95-PE (clone DX2), CD3-PacBlue (clone HIT3a),
From eBioscience: PD1-PE-Cy7 (clone eBio J105), LAG3-PE (clone 3DS223H), CD45RO-PE-Cy7 (clone UCHL1), CD45-PerCp-Cy5.5 (clone HI30),
From BD: CD45RA-FITC or BV711 (clone HI100), CCR7-BV421 (clone 150503), CD122-BV510 (clone Mik-β3), CD62L-BV605 (clone DREG-56), CD4-BUV395 (clone SK3), CD8-BUV805 (clone SK1).
Lynn R.C., Weber E.W., Sotillo E., Gennert D., Xu P., Good Z., Anbunathan H., Lattin J., Jones R., Tieu V., Nagaraja S., Granja J., DeBourcy C., Majzner R., Satpathy A.T., Quake S.R., Monje M., Chang H, & Mackall C.L. (2019). c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature, 576(7786), 293-300.
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