HSV-1 virus stock (VR-733) was purchased from ATCC (Manassas, VA). PRR agonists CpG (ODN2395), CL264 (cat#: tlrl-c264e), LPS (cat#: tlrl-b5LPS), Pam3CSK4 (cat#: tlrl-pms), PolydA:dT (cat#: tlrl-patc), PolyI:C-LMW (cat#:tlrl-picwlv), polyI:C-TLR3 agonist (cat#: tlrl-picw), and SS40 (cat#: tlrl-lrna40) were purchased from InvivoGen (San Diego, CA). Recombinant human TNFα (cat# 210-TA-020/CF), IL-1β (cat# 201-LB-005), IFNγ, IL-4, IL-22, GM-CSF, monoclonal mouse IgG1 Clone#11711 (Cat# MAB002), human IL-1β/IL-1F2 antibody (MAB601), human TNFαR1/TNFRSF1A antibody (MAB625), and anti-human IFNγR1 antibody (MAB6731) were bought from R&D systems, Inc. (Minneapolis, MN). Recombinant human IFNα (cat#:11101-1) and mouse anti-human IFNα (cat#: 21112-1) were purchased from PBL Biomedical Laboratories (Piscataway, NJ). Myc-DDK1-tagged RELA (RC220780), Myc-DDK1-tagged NFKB1 (RC208384), Myc-DDK1-tagged IRF7 (RC217934), Myc-DDK1-tagged ANKRD1 (RC205609) and Myc-DDK1-tagged STING were purchased from OriGene (Rockville, MD). Dr. Hong-Bing Shu (Wuhan University, China) kindly provided pCMV-flag-IRF3 and pCMV-flag-MyD88. PRK-neo-HA-ANKRD1 was generated in our lab by insertion of an encoding cDNA fragment in frame into PRK-neo-HA vector (a kind gift from Dr. Hong-Bing Shu).
Monoclonal mouse igg1 clone 11711
Manufactured by R&D Systems
Sourced in United States
Monoclonal mouse IgG1 Clone#11711 is a lab equipment product provided by R&D Systems. It is a monoclonal antibody of the IgG1 isotype derived from mouse.
Automatically generated - may contain errors
Lab products found in correlation
Tlr3 agonist poly 1 c, by InvivoGen (1 mentions)
Poly 1 c lmw, by InvivoGen (1 mentions)
Recombinant human tnf α, by R&D Systems (1 mentions)
Poly da dt, by InvivoGen (1 mentions)
Il 22, by R&D Systems (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Pam3csk4, by InvivoGen (1 mentions)
Gm csf, by R&D Systems (1 mentions)
Lipopolysaccharides (lps), by InvivoGen (1 mentions)
Ifn γ, by R&D Systems (1 mentions)
Chloroform, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Sodium bicarbonate, by Merck Group (1 mentions)
Deuterium oxide, by Merck Group (1 mentions)
2 protocols using monoclonal mouse igg1 clone 11711
1
Viral Infection and Immune Response Assay
2
Murine J591 mAb Conjugation with MIRB
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Murine J591 mAb, at a concentration of 5 mg/mL and with known specificity for PSMA, was procured from Dr. Neil H Bander's laboratory (Laboratory of Urological Oncology, Weill-Cornell Medical College, New York, NY, USA) through an institutional material transfer agreement. The isotype control antibody for synthesis and cell culture experiments was a murine IgG1 (Monoclonal Mouse IgG1 Clone# 11711; Cat #: MAB002, R&D systems, Minneapolis, USA) at a concentration of 5 mg/mL in 1× phosphate buffered saline without calcium or magnesium ions (Cat #: SH30028.02, Hyclone Inc, Logan, UT, USA), as per manufacturer's recommendation.
Molday ION Rhodamine Carboxyl (MIRB, Cat #: CL-50Q02-6C-50, BioPAL, Inc, Worcester, MA, USA) was used to prepare the antibody:SPIO complexes. All the reagents required for the conjugation reactions and analysis, including N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), 2-(4-Morpholino)ethanesulphonic acid hydrate (MES), sodium bicarbonate, HPLC-grade water (ChromoSolv Water), chloroform, acetonitrile, and deuterium oxide, were purchased from Sigma Aldrich, Oakville, ON, Canada, unless otherwise stated.
Molday ION Rhodamine Carboxyl (MIRB, Cat #: CL-50Q02-6C-50, BioPAL, Inc, Worcester, MA, USA) was used to prepare the antibody:SPIO complexes. All the reagents required for the conjugation reactions and analysis, including N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), 2-(4-Morpholino)ethanesulphonic acid hydrate (MES), sodium bicarbonate, HPLC-grade water (ChromoSolv Water), chloroform, acetonitrile, and deuterium oxide, were purchased from Sigma Aldrich, Oakville, ON, Canada, unless otherwise stated.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!