Log In Sign up
The largest database of trusted experimental protocols

Polyclonal antibodies

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom, United States

Polyclonal antibodies are a diverse collection of antibodies produced by multiple B-cell lineages in response to an antigen. They recognize multiple epitopes on the target antigen, providing a broad spectrum of reactivity. Polyclonal antibodies are commonly used in various immunoassays and research applications.

Automatically generated - may contain errors

Lab products found in correlation

Colloidal blue staining kit, by Thermo Fisher Scientific (2 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Anti a1at, by Merck Group (1 mentions) Sandwich elisa kit, by R&D Systems (1 mentions) Ecl reagent, by GE Healthcare (1 mentions) Handheld homogenizer, by Omni International (1 mentions) Quantity one software, by Bio-Rad (1 mentions) β actin, by Merck Group (1 mentions) Supersignal west pico, by Thermo Fisher Scientific (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Gapdh, by Abcam (1 mentions) β actin, by Abcam (1 mentions) Erk1 2 137f5, by Cell Signaling Technology (1 mentions) Sc 247, by Santa Cruz Biotechnology (1 mentions) Ecl plus kit, by GE Healthcare (1 mentions) Horseradish peroxidase coupled secondary antibody, by Abcam (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Vimentin, by Merck Group (1 mentions) Ibright fl1000, by Thermo Fisher Scientific (1 mentions) Phosphosafe, by Merck Group (1 mentions)

Close spelling variants of this product

Primary rabbit polyclonal antibodies (4 citations) Goat polyclonal anti‐BubR1 antibodies (3 citations) Rabbit anti-human C3b polyclonal antibodies (2 citations) 6x-His tag-specific polyclonal antibodies (2 citations) Anti‐flagellin polyclonal antibodies (cat no. ab93713; (2 citations) Rabbit anti-FASN polyclonal antibodies (2 citations) Goat polyclonal anti-type I collagen α1 antibodies (2 citations) Mouse polyclonal antibodies (2 citations) Polyclonal rabbit anti-GAP43 antibodies (2 citations) Polyclonal antibodies targeting OGT (2 citations)

15 protocols using polyclonal antibodies

1

Immunohistochemical Analysis of Murine Retinal GST3

Check if the same lab product or an alternative is used in the 5 most similar protocols
The enucleated mouse eyes were embedded whole in O.C.T. (Tissue Tek), frozen at −80°C, cryo-sectioned, and stored at −20°C. Retinal sections were fixed with 4% paraformaldehyde and processed using standard protocol for IHC by probing with polyclonal antibodies (Abcam, Inc.) against murine GST3/GST pi protein (murine homolog of GSTP1), followed by secondary antibodies coupled to Alexa 488 (Invitrogen) showing green fluorescence. DAPI was used to stain nuclei (blue). The immunostaining was detected using a confocal Leica TSP microscope.
Lee W.H., Joshi P, & Wen R. (2014). Glutathione S-Transferase Pi Isoform (GSTP1) Expression in Murine Retina Increases with Developmental Maturity. Advances in experimental medicine and biology, 801, 23-30.
+ Open protocol
+ Expand
2

Serum A1AT Glycoprotein Analysis

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Pooled, HCC serum [11 (link)] was depleted of IgG using protein A/G coated agarose beads as described elsewhere [10 (link)] and serum incubated on 96-well plates coated with A1AT antibody for 2 hours at room temperature. Captured proteins were resolved via SDS-PAGE and either stained with colloidal Coomassie brilliant blue (Colloidal Blue Staining Kit, Thermo Fisher) or proteins transferred using western-blotting method to PVDF membranes for immunoblot analysis. Fucosylation was detected using biotin-conjugated Aleuria aurantia lectin (AAL). A1AT was detected using a polyclonal anti-A1AT (Sigma-Aldrich). IgM or IgG was detected using polyclonal antibodies (Abcam, Cambridge, MA). Bound AAL or antibody was visualized using IRDye®800-conjugated streptavidin or IRDye®700-conjugated anti-rabbit antibody.
Wang M., Comunale M.A., Herrera H., Betesh L., Kono Y, & Mehta A. (2016). Identification of IgM as a contaminant in lectin-FLISA assays for HCC detection. Biochemical and biophysical research communications, 476(3), 140-145.
+ Open protocol
+ Expand
3

Serum Enzyme and Antioxidant Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum activity of enzymes creatine kinase (CK) and lactate dehydrogenase (LDH) was measured with commercial kits (Labtest, São Paulo, Brazil). Total reactive antioxidant potential (TRAP) was determined as described in the literature [37 (link)]. The assay is based on the employment of a peroxyl radical generator (2,2-azo-bis(2-amidinopropane); AAPH) mixed with luminol, and the scavenging activity of samples prevents luminol oxidation by AAPH. The synthetic antioxidant Trolox (Acros Organics BVBA, Geel, Belgium), a vitamin E analog, was applied as a positive control at a concentration of 100 µM [38 (link)]. The antioxidant capacity of samples was recorded through 60 min and results were calculated as area under the curve (AUC). Quantitative analysis of IL-1β and IL-10 was determined by indirect ELISA using polyclonal antibodies (Abcam, Cambridge, UK). TNF-α was quantified using an ELISA sandwich kit following the manufacturer’s instructions (R&D Systems, Inc., Minneapolis, MN, USA).
Petiz L.L., Girardi C.S., Bortolin R.C., Kunzler A., Gasparotto J., Rabelo T.K., Matté C., Moreira J.C, & Gelain D.P. (2017). Vitamin A Oral Supplementation Induces Oxidative Stress and Suppresses IL-10 and HSP70 in Skeletal Muscle of Trained Rats. Nutrients, 9(4), 353.
+ Open protocol
+ Expand
4

Immunological Profiling of Host Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Specimens from host spleen, lung and colon were mechanically disrupted and homogenized using a handheld homogenizer (Omni International, Kennesaw, GA). Supernatants were collected for ELISA of tumor necrosis alpha (TNF-α), interferon gamma (IFN-γ), RANTES/CCL-5 and phosphorylated STAT 5A/B (eBioscience, San Diego, CA). Inducible nitric oxide synthase (iNOS) levels were detected using polyclonal antibodies (Abcam, Cambridge, UK) for western blotting. Total protein concentration in the supernantant was measured using Bradford’s reagent. Supernatants were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were then transferred from the gel to nitrocellulose membranes and ECL reagent was used for visualization of the protein (GE Healthcare, Buckinghamshire, UK).
Robles J.D., Liu Y.P., Cao J., Xiang Z., Cai Y., Manio M., Tang E.H, & Chan G.C. (2015). Immunosuppressive mechanisms of human bone marrow derived mesenchymal stromal cells in BALB/c host graft versus host disease murine models. Experimental Hematology & Oncology, 4, 13.
+ Open protocol
+ Expand
5

Klotho and MnSOD Expression in Kidney

Check if the same lab product or an alternative is used in the 5 most similar protocols
The protein expression of Klotho and manganese superoxide dismutase (MnSOD) in kidney homogenates was determined by western blot analysis. The polyclonal antibodies for Klotho (1:1000) and MnSOD (1:50) were obtained from Abcam (Cambridge, MA) and Cell Signaling Technology (Danvers, MA). Kidney homogenates were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes, and blocked overnight at 4°C in 5% bovine serum albumin. Immunodetection was performed by incubating the membranes with the primary antibodies diluted in blocking buffer for 1 h at room temperature. After washing, a chemiluminescent horseradish peroxidase substrate was diluted in blocking buffer and applied for 60 min. Band intensity was quantified with the Quantity One software (Bio-Rad, Hercules, CA), with β-Actin acting as the normalization protein (1:10,000; Sigma-Aldrich, St. Louis, MO).
Ali M.F., Venkatarayappa S.K., Benny M., Rojas C., Yousefi K., Shehadeh L.A., Kulandavelu S., Sharma M., Da Silva N., Freundlich M., Abitbol C.L., DeFreitas M.J, & Young K.C. (2020). Effects of Klotho supplementation on hyperoxia-induced renal injury in a rodent model of postnatal nephrogenesis. Pediatric research, 88(4), 565-570.
+ Open protocol
+ Expand
6

Immunodetection of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyclonal antibodies against the housekeeping gene β-actin and GAPDH were purchased from Abcam and Santa Cruz Biotechnology, respectively. Histone H3 and phospho-ERK1/2 (Thr 202/Tyr 204) (20G11) were purchased from Cell Signaling Technology (Beverly, MA). Antibodies to the phosphorylated forms of cyclin E (sc-12917-R), CDK (sc-28435-R), CDK-2 (sc-101656), total form of cyclin E (sc-247), and viral protein ICP0 (sc-56985) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), Us11 antibody was kindly provided by Professor Bernard Roizman. ERK1/2 (137F5) was purchased from Cell Signaling Technology. Monoclonal antibody to FLAG FITC-conjugate was purchased from SIGMA. Secondary antibodies anti-rabbit and anti-mouse IgG conjugated to peroxidase were also from Santa Cruz Biotechnology. Protein bands were visualized using SuperSignal West Pico as a chemiluminescent substrate (Thermo Scientific, Rockford, IL).
Colao I., Pennisi R., Venuti A., Nygårdas M., Heikkilä O., Hukkanen V, & Sciortino M.T. (2017). The ERK-1 function is required for HSV-1-mediated G1/S progression in HEP-2 cells and contributes to virus growth. Scientific Reports, 7, 9176.
+ Open protocol
+ Expand
7

Protein Immunoblot Analysis Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Specific proteins or human serum[23 (link)] depleted as above were resolved via SDS-PAGE and either stained with colloidal Coomassie brilliant blue (Colloidal Blue Staining Kit, Thermo Fisher) or transferred PVDF membranes for immunoblot analysis. IgM, IgG, or kininogen was detected using polyclonal antibodies (Abcam, Cambridge, MA and AbBiotec, San Diego, CA, GenScript, Piscataway, NJ). Bound antibody was visualized using IRDye® 800-conjugated anti-mouse-800, IRDye®-conjugated anti-mouse-antibody, IRDye® 800-conjugated anti-goat antibody.
Wang M., Shen J., Herrera H., Singal A., Swindell C., Renquan L, & Mehta A. (2018). Biomarker analysis of fucosylated kininogen through depletion of lectin reactive heterophilic antibodies in hepatocellular carcinoma.. Journal of immunological methods, 462, 59-64.
+ Open protocol
+ Expand
8

Immunolocalization of Occludin and ZO-1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence was used to determine the localization of occludin and, zona occludens-1 (ZO-1) proteins. The intestinal section was washed with PBS, mounted in embedding medium, and stored at −80 °C until use. Frozen sections were cut at 10 μm and were mounted on the slides. The polyclonal antibodies against occludin and, ZO-1 (Abcam, Cambridge, UK) were incubated according to the manufacturer’s instructions. The sections were probed with their respective FITC-conjugated secondary IgG antibodies. The nuclei were counterstained with DAPI (4, 6-diamidino-2-phenylindole). Slides incubated without any primary antibody were using as negative controls. Confocal analysis was performed with a confocal scanning microscope (Leica Microsystems, Heidelberg GmbH, Mannheim, Germany).
Hu Q., Ren H., Ren J., Liu Q., Wu J., Wu X., Li G., Wang G., Gu G., Guo K., Hong Z., Liu S, & Li J. (2018). Released Mitochondrial DNA Following Intestinal Ischemia Reperfusion Induces the Inflammatory Response and Gut Barrier Dysfunction. Scientific Reports, 8, 7350.
+ Open protocol
+ Expand
9

Immunohistochemical Analysis of Dystrophin and Sarcoglycan Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Histopathological studies were performed as before (33 (link), 34 (link)). Polyclonal antibodies against DMD (Abcam, Cambridge, UK) and SGCD (R214), and monoclonal antibodies against α-DG (IIH6), β-DG (AP83), SGCA (20A6), and SGCG (21B5) were used for immunohistochemistry.
Turk R., Hsiao J.J., Smits M.M., Ng B.H., Pospisil T.C., Jones K.S., Campbell K.P, & Wright M.E. (2016). Molecular Signatures of Membrane Protein Complexes Underlying Muscular Dystrophy. Molecular & Cellular Proteomics : MCP, 15(6), 2169-2185.
+ Open protocol
+ Expand
10

Quantification of VEGFA Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in PhosphoSafe (MERCK Life Sciences, Billerica, MA, USA). Equal amounts of protein isolates were separated on SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA). For VEGFA protein expression, we used 1 mg/mL polyclonal antibodies (Abcam, Cambridge, UK). Vimentin (Sigma-Aldrich, Saint Louis, MS, USA) served as loading control. For visualization, we used horseradish peroxidase-coupled secondary antibodies (Abcam and Agilent) and the ECL Plus kit (GE Healthcare, Chalfont St. Giles, UK). iBright FL1000 (Thermo Fisher, Waltham, MS, USA) was used for processing and visualization. All western blots were repeated three times showing comparable results.
Krebs M., Kotlyar M.J., Fahl J., Janaki Raman S., Röhrig F., Marquardt A., Kübler H., Kneitz B., Schulze A, & Kalogirou C. (2023). Metformin Regulates the miR-205/VEGFA Axis in Renal Cell Carcinoma Cells: Exploring a Clinical Synergism with Tyrosine Kinase Inhibitors. Urologia Internationalis, 108(1), 49-59.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!