Quantikine elisa human c reactive protein crp immunoassay kit

Frozen plasma was thawed and used to measure Ox-LDL, ET-1, VEGF-A and creatinine using commercial kits. Each assay was performed according to the manufacturer’s instructions. Ox-LDL was used as a marker of oxidative stress, using a competitive ELISA kit, (Mercodia, Diagenics, Milton Keynes, UK) [21 (link)]. ET-1, assayed with a solid phase Quantikine ELISA kit (R&D systems, Abingdon, UK) and VEGF-A, measured using a Platinum ELISA, (eBioscience, Hatfield, UK) were used as markers of endothelial dysfunction. Plasma CRP levels were measured using the Quantikine ELISA Human C reactive protein/CRP immunoassay kit (R&D systems), according to the manufacturer’s instructions.
Samples were corrected for serum creatinine level using a colorimetric assay, (Cayman Medical, Cambridge Bioscience, UK).

To measure the inflammation level, 5 cc of blood was drawn from each participant’s forearm between 8 A.M. and 10 A.M. after an 8 h fast. The collected blood specimen was immediately put in the EDTA tube treated with citric acid, shaken over 20 times to prevent clotting, and transported in an icebox to a laboratory. Then, it was centrifuged for 15 min at 3000 rpm, and the separated plasma supernatant was moved into 2.0 mL micro-tubes and kept at −70℃. IL-6 and CRP were measured using Microplate Reader (VERSA Max, USA) following the method of Enzyme-Linked Immunosorbent Assay (ELISA). The reagents used at the time of analysis were Quantikine ELISA Human IL-6 Immunoassay kit (R&D, Torrance, CA, USA) and Quantikine ELISA Human C-Reactive Protein/CRP Immunoassay kit (R&D, USA).