Procyanidin b2

Manufactured by Cayman Chemical

Sourced in United States

Procyanidin B2 is a polyphenolic compound used in biochemical research. It functions as a standard reference material for the quantification and identification of similar compounds.

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Lab products found in correlation

4 protocols using procyanidin b2

Comprehensive Anticancer Drug Protocol

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Chemotherapeutics, 5-FU (S1224), gemcitabine (S1149) and irinotecan (S2217), were purchased from Selleckchem (Houston, TX, USA). Targeted cancer therapy drugs, gefitinib (S1025) and selumetinib (S1008) were purchased from Selleckchem, while lapatinib (11493) was purchased from Cayman Chemical (Ann Harbor, MI, USA). Fulvestrant (S1191, Selleckchem) and metformin (13118, Cayman Chemical) were used as repurposed agents. Natural products, apigenin (S2262), curcumin (S1848), fisetin (S2298), and forskolin (S2449) were purchased from Selleckchem, while (-)- epigallocatechin gallate (70935), procyanidin B2 (19865), resveratrol (70675) and urolithin A (22607) were purchased from Cayman Chemical. All chemicals had more than 98% purity. Stock solutions were prepared according to the manufacturers’ instructions.

Kucukkaraduman B., Cicek E.G., Akbar M.W., Demirkol Canli S., Vural B, & Gure A.O. (2021). Epithelial-to-Mesenchymal Transition Is Not a Major Modulating Factor in the Cytotoxic Response to Natural Products in Cancer Cell Lines. Molecules, 26(19), 5858.

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Procyanidin B2 Hydrolysis Protocol

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Procyanidin B2 (CAS number 29 106 – 49 – 8) with purity ≥ 98% was purchased from Cayman Chemical Company. The reference standard Procyanidin B2 was hydrolyzed in the reference solution to form the reference peak cyanidin
in situ(see below).

Kulić Ž., Ritter T., Röck B., Elsäßer J., Schneider H, & Germer S. (2021). A Detailed View on the Proanthocyanidins in Ginkgo Extract EGb 761. Planta Medica, 88(5), 398-404.

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Phytochemicals for Cancer Research

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The reference phytochemicals, including isorhamnetin 3-O-glucoside, kaempferol 3-O-glucuronide, and quercetin 3-O-glucuronide were obtained from Extrasynthese (Genay, France). Gallic acid, catechin, and epicatechin were obtained from Carl Roth (Karlsruhe, Germany). Procyanidin B1, procyanidin B2, procyanidin B3, and procyanidin C1 were purchased from Cayman Chemical (Ann Arbor, MI, USA), while agrimoniin, apigenin, 3-O-caffeoylquinic acid, ellagic acid, astragalin (kaempferol 3-O-glucoside), pedunculagin, avicularin (quercetin 3-O-arabinoside), hyperoside (quercetin 3-O-galactoside), isoquercitrin (quercetin 3-O-glucoside), and tiliroside (purity > 96%) were previously isolated in the Department of Pharmacognosy of Medical University of Białystok (Białystok, Poland) [22 (link),48 ,49 (link),50 (link),51 (link)]. All other analytical grade chemicals used in the study were obtained from Sigma-Aldrich (St. Louis, MO, USA). To obtain ultra-pure water, a POLWATER DL3-100 Labopol (Kraków, Poland) system was used. Investigated extracts (100 mg/mL) and 5-fluorouracil (50 mM) were dissolved in dimethyl sulfoxide (DMSO) to prepare stock solutions. Working solutions were prepared by dissolving stock solutions in a culture medium. The final concentration of DMSO in all working solutions used in the studies was 0.25%.

Augustynowicz D., Lemieszek M.K., Strawa J.W., Wiater A, & Tomczyk M. (2023). Phytochemical Profiling of Extracts from Rare Potentilla Species and Evaluation of Their Anticancer Potential. International Journal of Molecular Sciences, 24(5), 4836.

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Phytochemical Analysis of Antioxidant Compounds

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2,2-Diphenyl-1-picrylhydrazyl (DPPH), Folin–Ciocalteu reagent, formic acid, and sorbitol were purchased from Sigma Aldrich (St. Louis, MO). Spherical silica particles (surface area: 270–300 m²/g, particle size: 75–200 μm, total pore volume: 0.7–0.9 cm³/g, as specified by the manufacturer) were also obtained from Sigma Aldrich (St. Louis, MO, USA). Sodium carbonate, sodium chloride, HPLC-grade acetonitrile and methanol, hydrochloric acid, sulfuric acid, sodium hydroxide, and hydrogen peroxide were purchased from Fischer Scientific Co. (Pittsburgh, PA, USA). Methoxy polyethylene glycol Silane-2000 and -5000 (mPEG–silane) were purchased from Laysan Bio (Arab, AL). Analytical reference materials were obtained as follows: Gallic acid, (+)-catechin, delphinidin chloride, p-coumaric acid, quercetin, and kaempferol were supplied from Millipore Sigma (St. Louis, MO, USA); procyanidin B2, isoquercetin, malvidin chloride, and myricetin were purchased from Cayman Chemical (Ann Arbor, MI, USA). Water was purified using a Barnstead E-Pure system (ThermoFisher Scientific, Waltham, MA, USA) with a resistivity of 18 MΩ-cm unless otherwise specified.

Seker A., Arslan B, & Chen S. (2019). Recovery of Polyphenols from Grape Pomace Using Polyethylene Glycol (PEG)-Grafted Silica Particles and PEG-Assisted Cosolvent Elution. Molecules, 24(12), 2199.

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