IHC and IF analyses were performed as described previously (49 (link)). Primary antibodies for IHC and IF were as followed: anti-MUC2 (GB14110; 1:500; Servicebio); anti-MPO (GB12224; 1:200; Servicebio); anti-Occludin (GB111401">GB111401 ; 1:200; Servicebio). Data were analyzed by Image J.
Gb14110
Manufactured by Wuhan Servicebio Technology
Sourced in China
The GB14110 is a laboratory equipment designed for general use in scientific research and analysis. It serves as a versatile tool for various experimental purposes. The core function of the GB14110 is to provide a controlled environment for various laboratory procedures and experiments. No further details on its intended use can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Gb12224, by Wuhan Servicebio Technology (2 mentions)
Anti muc2, by Wuhan Servicebio Technology (2 mentions)
Anti mpo, by Wuhan Servicebio Technology (2 mentions)
Anti occludin, by Wuhan Servicebio Technology (2 mentions)
Gb111401, by Wuhan Servicebio Technology (2 mentions)
Gb111402, by Wuhan Servicebio Technology (2 mentions)
Gb11027, by Wuhan Servicebio Technology (1 mentions)
Gb11108, by Wuhan Servicebio Technology (1 mentions)
Gb113497, by Wuhan Servicebio Technology (1 mentions)
Fd0129, by Fdbio (1 mentions)
Bs 20747r, by Bioss Antibodies (1 mentions)
Pannoramic desk, by 3DHISTECH (1 mentions)
4 protocols using gb14110
1
Immunohistochemical and Immunofluorescence Analysis
2
Immunohistochemical and Immunofluorescence Analysis
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IHC and IF analyses were performed as described previously (49 (link)). Primary antibodies for IHC and IF were as followed: anti-MUC2 (GB14110; 1:500; Servicebio); anti-MPO (GB12224; 1:200; Servicebio); anti-Occludin (GB111401">GB111401 ; 1:200; Servicebio). Data were analyzed by Image J.
3
Pathological and Immunohistochemical Analysis of Colon Tissues
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Colon tissues were formalin fixed overnight at room temperature and then embedded in paraffin. 5 µm sections were stained with hematoxylin and eosin (HE) for pathological analysis. The pathological score was assessed by the severity of inflammation, the degree of inflammatory involvement, and the degree of epithelial/crypt damage as previously reported.58 (link) Calculate each parameter and sum to get the total score.
For immunohistochemistry, paraffin-embedded tissue sections were stained with anti-MUC2 antibody (GB14110, 1:1000, Servicebio, China), anti-ZO-1 antibody (GB111402, 1:1000, Servicebio, China) and visualized by DAB staining according to manufacturer’s instructions.
For immunofluorescence, paraffin-embedded tissues were stained with anti-F4/80 antibody (GB11027, 1:1000, Servicebio, China), anti-Il-10 antibody (GB11108, 1:1000, Servicebio, China) and anti-CD206 antibody (GB113497, 1:500, Servicebio, China). The slides were independently assessed by two researchers who were unaware of the nature of the samples.
For immunohistochemistry, paraffin-embedded tissue sections were stained with anti-MUC2 antibody (GB14110, 1:1000, Servicebio, China), anti-ZO-1 antibody (GB111402, 1:1000, Servicebio, China) and visualized by DAB staining according to manufacturer’s instructions.
For immunofluorescence, paraffin-embedded tissues were stained with anti-F4/80 antibody (GB11027, 1:1000, Servicebio, China), anti-Il-10 antibody (GB11108, 1:1000, Servicebio, China) and anti-CD206 antibody (GB113497, 1:500, Servicebio, China). The slides were independently assessed by two researchers who were unaware of the nature of the samples.
4
Colon Tissue Analysis: Morphological and Molecular Insights
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Colon tissues were fixed in 4% paraformaldehyde. Hematoxylin and eosin (H&E) staining and Periodic acid Schiff (PAS) staining were conducted to assess morphological changes. The paraffin-embedded colon tissues were deparaffinized, rehydrated, retrieved, and antigen retrieval. Tissues were sealed with 3% bovine serum albumin and incubated with primary antibodies: anti-Muc2 antibody (Servicebio, GB14110, 1:500), anti-ZO1 antibody (Servicebio, GB111402, 1:500), anti-Occludin antibody (Servicebio, GB111401, 1:500), anti-Lgr5 (Bioss, bs-20747R,1:100). The slices were then incubated with secondary antibodies (Fdbio, FD0129, 1:500; Fdbio, FD0136,1:500). The images were scanned with Pannoramic Scanner (Pannoramic DESK, 3DHISTECH) and observed with Caseviewer C.V 2.3.
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