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Digital just for mouse stereotaxic instrument

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The Digital Just for Mouse Stereotaxic Instrument is a precision instrument designed for neuroscience research on mice. It provides a stable platform for accurately positioning and securing the mouse's head during procedures.

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Lab products found in correlation

Model s48 stimulator, by Natus (3 mentions)

Close spelling variants of this product

Digital Mouse Stereotaxic Instrument Digital stereotaxic instrument Mouse stereotaxic instrument Stereotaxic instrument

4 protocols using digital just for mouse stereotaxic instrument

1

Anesthesia and Implantation of Telemetry Devices in Mice

Cited 1 time
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Mice were anesthetized with ketamine/xylazine (80/10 mg/kg, i.p.) and placed in a stereotaxic instrument (Digital Just for Mouse Stereotaxic Instrument, Stoelting). Ophthalmic ointment (Puralube, Dechra) was applied. Post-surgery mice received subcutaneous sterile saline injections to prevent dehydration and an analgesic (buprenorphine; 0.1 mg/kg, i.p.). G2 E-Mitters were implanted intraperitoneally as described [35 (link)].
Province H.S., Xiao C., Mogul A.S., Sahoo A., Jacobson K.A., Piñol R.A., Gavrilova O, & Reitman M.L. (2020). Activation of neuronal adenosine A1 receptors causes hypothermia through central and peripheral mechanisms. PLoS ONE, 15(12), e0243986.
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2

Stereotaxic Injection and Anesthesia Protocol

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Mice were anesthetized with 0.5–1.5% isoflurane (1 L per minute of oxygen) or a ketamine/xylazine mix (80/10 mg/kg) and placed in a stereotaxic instrument (Digital Just for Mouse Stereotaxic Instrument, Stoelting). Ophthalmic ointment (Puralube, Dechra) was applied. All injections were done with pulled-glass pipettes (pulled 20–40 m tip diameter; 0.275 ID, 1 mm OD, Wilmad Lab Glass ) at a visually controlled rate of 50 nl per min with an air pressure system regulator (Grass Technologies, Model S48 Stimulator). The pipette was kept in place for 5 min after injection. Post-surgery mice received subcutaneous sterile saline injections to prevent dehydration and analgesic (buprenorphine; 0.1 mg/kg).
Piñol R.A., Zahler S.H., Li C., Saha A., Tan B.K., Škop V., Gavrilova O., Xiao C., Krashes M.J, & Reitman M.L. (2018). Brs3 neurons in the mouse dorsomedial hypothalamus regulate body temperature, energy expenditure and heart rate, but not food intake. Nature neuroscience, 21(11), 1530-1540.
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3

Stereotaxic Injection and Anesthesia Protocol

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Mice were anesthetized with 0.5–1.5% isoflurane (1 L per minute of oxygen) or a ketamine/xylazine mix (80/10 mg/kg) and placed in a stereotaxic instrument (Digital Just for Mouse Stereotaxic Instrument, Stoelting). Ophthalmic ointment (Puralube, Dechra) was applied. All injections were done with pulled-glass pipettes (pulled 20–40 m tip diameter; 0.275 ID, 1 mm OD, Wilmad Lab Glass ) at a visually controlled rate of 50 nl per min with an air pressure system regulator (Grass Technologies, Model S48 Stimulator). The pipette was kept in place for 5 min after injection. Post-surgery mice received subcutaneous sterile saline injections to prevent dehydration and analgesic (buprenorphine; 0.1 mg/kg).
Piñol R.A., Zahler S.H., Li C., Saha A., Tan B.K., Škop V., Gavrilova O., Xiao C., Krashes M.J, & Reitman M.L. (2018). Brs3 neurons in the mouse dorsomedial hypothalamus regulate body temperature, energy expenditure and heart rate, but not food intake. Nature neuroscience, 21(11), 1530-1540.
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4

Stereotaxic Brain Injections in Mice

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Mice were anesthetized with 0.5–1.5% isoflurane (1 L/min oxygen) or a ketamine/xylazine mix (80/10 mg/kg, i.p.), placed in a stereotaxic instrument (Digital Just for Mouse Stereotaxic Instrument, Stoelting), and ophthalmic ointment (Puralube, Dechra) was applied. Brain injections were done with pulled-glass pipettes (pulled 20–40 μm tip diameter; 0.275 mm ID, 1 mm OD, Wilmad Lab Glass) at a visually controlled rate of ~50 nl per min with an air MAP system regulator (Grass Technologies, Model S48 Stimulator). The pipette was kept in place for 5 min after injection. Post-surgery, mice received subcutaneous saline injections to prevent dehydration and analgesic (buprenorphine, 0.1 mg/kg, s.c.).
Piñol R.A., Mogul A.S., Hadley C.K., Saha A., Li C., Škop V., Province H.S., Xiao C., Gavrilova O., Krashes M.J, & Reitman M.L. (2021). Preoptic BRS3 neurons increase body temperature and heart rate via multiple pathways. Cell metabolism, 33(7), 1389-1403.e6.
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