In vivo NJP ganglion imaging was performed as described previously (Williams et al., 2016 (link)). Briefly, Piezo2-GCaMP* and P2ry1-GCaMP* mice were anesthetized by isoflurane inhalation and the left ganglion was surgically exposed after severing central connections and immobilized on a stable platform for confocal imaging (Leica SP5 II) of calcium transients. Stretch of the lower airways was achieved by gas infusion of (1 liter/min, 15 sec) through a tracheal cannula (PE10 tubing, Braintree Scientific) inserted below the thyroid cartilage and advanced to the level of the carina. The baseline activity for each neuron was defined as the average GCaMP3 fluorescence intensity over a three-minute period preceding stimulus delivery, and cells were excluded if they failed to display maximal responses to electrical stimulation greater than seven standard deviations above their baseline means at the conclusion of the experiment. Cells were identified as responsive if (1) maximum GCaMP3 fluorescence exceeded seven standard deviations above the baseline mean, or (2) mean GCaMP3 fluorescence during the stimulation period exceeded three standard deviations above the baseline mean. Response analysis was performed blind to neuron identity.
Pe10 tubing
Manufactured by Braintree Scientific
PE10 tubing is a type of polyethylene tubing commonly used in various scientific and laboratory applications. It is a thin, flexible, and transparent tubing with an inner diameter of approximately 0.28 mm and an outer diameter of approximately 0.61 mm. PE10 tubing is resistant to many chemicals and can withstand a range of temperatures, making it suitable for a variety of laboratory tasks.
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Lab products found in correlation
4 protocols using pe10 tubing
1
In vivo NJP Ganglion Calcium Imaging
2
FeCl3-Induced Carotid Thrombosis Lysis
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Thrombolysis was assessed in mice subjected to FeCl3 carotid artery thrombosis. After 5 consecutive minutes of vessel occlusion, mice were infused with Tenecteplase (5 mg/kg, generous gift of Genentech, CA) through a saphenous vein intravenous catheter constructed of pulled PE-10 tubing (Braintree Scientific, Braintree, MA) with a 3·0-mil (0·076-mm diameter) cleaning wire (Hamilton Company, Reno NV) placed into the lumen as a stylet, as described (Machlus et al., 2011a (link)), while continuously monitoring carotid blood flow. Blood was sampled from the IVC 5 min after the return of blood flow or 30 min after Tenecteplase infusion if the clot did not lyse.
3
Intracerebral Contrast Agent Infusion
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Just prior to imaging, rats were anaesthetized with 2–3% isoflurane and received a subcutaneous injection of the analgesic Metacam (Meloxicam, 5 mg/mL solution) in a dose of 1 mg/kg. The scalp was incised, and a burr hole made in the skull for implantation of sterile PE10 tubing (Braintree Scientific) aimed at the right lateral cerebroventricle using the stereotaxic coordinates: 1.0 mm posterior to the bregma, 2.0 mm lateral to the midline, and 4.0 mm in depth from dura as previously described.19 (link) Tubing prefilled with gadobenate dimeglumine (MultiHance®) 1.06 kD, contrast agent diluted at 1:20, was fixed in place with cyanoacrylic cement allowing for infusion outside the bore of the magnet. This injection method has been used in previous studies to deliver drugs directly to the brain during awake imaging.33 (link),34 (link)
4
Intracerebroventricular Contrast Delivery for Imaging
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Just prior to imaging, rats were anesthetized with 2 to 3% isoflurane and received an s.c. injection of the analgesic Metacam (meloxicam, 5 mg/mL solution) at a dose of 1 mg/kg. The scalp was incised and a burr hole was made in the skull for implantation of sterile PE10 tubing (Braintree Scientific) aimed at the right lateral cerebroventricle using the stereotaxic coordinates 1.0 mm posterior to the bregma, 2.0 mm lateral to the midline, and 4.0 mm in depth from dura. The tubing, ca 60 cm in length and prefilled with gadobenate dimeglumine (MultiHance) 1.06-kDa contrast agent (CA) diluted 1:20, was fixed in place with cyanoacrylic cement and connected to a 0.3-mL syringe needle filled with the contrast agent that could be positioned just outside the bore of the magnet. This injection method has been used in previous studies to deliver drugs directly to the brain during awake imaging (22 (link), 23 (link)). The surgery on rats maintained on the reversed light–dark cycle was performed under red-light illumination.
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