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2 protocols using glyceryl trilinolenate

1

Comprehensive Fatty Acid Profiling Protocol

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A fatty acid methyl ester (FAME) standard mixture containing all relevant fatty acid methyl esters was obtained from USP (Rockville, MD). Ammonium acetate (LC-MS grade) was purchased from Honeywell (Muskegon, MI). Glyceryl tricaprylate, glyceryl tricaprate, glyceryl trimyristate, glyceryl trilaurate, glyceryl tripalmitate, and glyceryl tripalmitoleate were acquired from Santa Cruz biotechnology (Dallas, TX). Glyceryl tristearate, glyceryl trioleinate, glyceryl trilinoleate, glyceryl trilinolenate, sodium hydroxide, 2-dimethylaminoethanol (DMAE), butylated hydroxytoluene (BHT), toluene, and certified analytical standards of cottonseed oil, sunflower seed oil, palm oil, coconut oil, canola oil, linseed oil, olive oil, corn oil, and soybean oil were obtained from Sigma-Aldrich (St. Louis, MO). Optima LC/MS grade acetonitrile and water were purchased from Fisher Scientific (Pittsburgh, PA).
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2

Comparative Analysis of Edible Oils

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Linseed oil (Lo), sunflower oil (So) and olive oil (Oo), were purchased from a local market (Tuscany, Italy). Methyl linolenate (Ln), methyl linoleate (L), methyl oleate (O), glyceryl trioleate (OOO), glyceryl trilinoleate (LLL), and glyceryl trilinolenate (LnLnLn) were purchased from Sigma-Aldrich (purity ≥ 98%).
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