Anti complex 2 70 kda fp subunit

Manufactured by Thermo Fisher Scientific

The Anti-Complex II 70 kDa Fp Subunit is a laboratory product used for research purposes. It functions as an antibody that specifically targets the 70 kDa Fp subunit of Mitochondrial Complex II, also known as Succinate Dehydrogenase. This antibody can be utilized in various analytical techniques to detect and study the 70 kDa Fp subunit of Mitochondrial Complex II.

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Lab products found in correlation

Wb blocking reagent, by Roche (2 mentions) Anti ndufa9, by Thermo Fisher Scientific (2 mentions) Anti ndufb8, by Thermo Fisher Scientific (2 mentions) Anti uqcrc1, by Thermo Fisher Scientific (2 mentions) Anti complex 4, by Thermo Fisher Scientific (2 mentions) Anti calnexin, by Merck Group (2 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti pakt s473, by Cell Signaling Technology (1 mentions) Anti glp 1r, by Novus Biologicals (1 mentions) Anti glut1, by Merck Group (1 mentions) Anti fgf21, by R&D Systems (1 mentions) Anti β actin, by Merck Group (1 mentions)

2 protocols using anti complex 2 70 kda fp subunit

Western Blotting Procedure for Protein Analysis

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Western blot analysis was conducted as previously described (Timper et al., 2017 (link)). In brief, membranes were blocked with 10% WB-blocking reagent (Roche, Switzerland) in Tris-buffered saline containing 0.1% Tween-20 (TBS-T), and incubated with primary antibodies, made up in TBS-T (0.1% Tween-20) and 5% Western Blotting-Reagent, at 4°C overnight. The following primary antibodies and dilutions were used: anti-GLP-1R (1:1000, #NBP1-97308, Novus Biologicals), anti-Akt (#4686, Cell Signaling), anti-p-Akt(S473) (#4060, Cell Signaling), anti-NDUFA9 (1:1000, #459100, Invitrogen), anti-NDUFB8 (1:1000, #459210, Invitrogen), anti-Complex II 70 kDa Fp Subunit (1:1000, #459200, Invitrogen), anti-UQCRC1 (1:1000, #459140, Invitrogen), anti-Complex IV (1:1000, #459110, Invitrogen), anti-ATP synthase subunit β (1:1000, #21351, Invitrogen), anti-p-Ser-226-GLUT-1 (#ABN991, Merck Millipore), anti-GLUT-1 (#07-1401, Merck Millipore), anti-FGF21 (#AF3057, R&D Systems) and, as loading control: anti-calnexin (1:4000, #208880, Merck Milipore). Quantification of chemiluminescent signals was performed with the ImageJ (Fiji)-software package (Schneider et al., 2012 (link)).

Timper K., del Río-Martín A., Cremer A.L., Bremser S., Alber J., Giavalisco P., Varela L., Heilinger C., Nolte H., Trifunovic A., Horvath T.L., Kloppenburg P., Backes H, & Brüning J.C. (2020). GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function. Cell Metabolism, 31(6), 1189-1205.e13.

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Western Blot Analysis of Mitochondrial Proteins

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Western blot analysis was conducted as previously described (Timper et al., 2017 (link)). In brief, membranes were blocked with 10% WB-blocking reagent (Roche, Switzerland) in Tris-buffered saline containing 0.1% Tween-20 (TBS-T), and incubated with primary antibodies, made up in TBS-T (0.1% Tween 20) and 5% Western Blotting-Reagent, at 4°C overnight. The following primary antibodies and dilutions were used: anti-AIF (1:1000, #ab32516, Abcam), anti-NDUFA9 (1:1000, #459100, Invitrogen), anti-NDUFB8 (1:1000, #459210, Invitrogen), anti-Complex II 70 kDa Fp Subunit (1:1000, #459200, Invitrogen), anti-UQCRC1 (1:1000, #459140, Invitrogen), anti-Complex IV (1:1000, #459110, Invitrogen), anti-ATP synthase subunit β (1:1000, #21351, Invitrogen) and, as loading control: anti-β-actin (1:2000, #A2228, Sigma Aldrich) or anti-calnexin (1:4000, #208880, Merck Milipore). Quantification of chemiluminescent signals was performed with the ImageJ (Fiji)-software package (Schneider et al., 2012 (link)).

Timper K., Paeger L., Sánchez-Lasheras C., Varela L., Jais A., Nolte H., Vogt M.C., Hausen A.C., Heilinger C., Evers N., Pospisilik J.A., Penninger J.M., Taylor E.B., Horvath T.L., Kloppenburg P, & Brüning J.C. (2018). Mild Impairment of Mitochondrial OXPHOS Promotes Fatty Acid Utilization in POMC Neurons and Improves Glucose Homeostasis in Obesity. Cell reports, 25(2), 383-397.e10.

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