For the small RNA sequencing, total RNA was isolated from tissue samples by using miRVana RNA isolation kit (ThermoFisher Scientific, Waltham, MA) following the total RNA procedure. RNA was eluted using RNase-free water and stored at − 80 °C. RNA from serum was isolated using the Qiagen Plasma/Serum kit (Qiagen, Hilden, Germany) using 200 μL of serum.
For the miRNA and mRNA microarray experiments, total RNA was isolated with TRIzol reagent according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA).
Exosomes were isolated from serum using size exclusion chromatography following the protocol of Böing et al. [32 ]. RNA was subsequently isolated using the above mentioned Qiagen Plasma/Serum kit.
For the miRNA and mRNA microarray experiments, total RNA was isolated with TRIzol reagent according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA).
Exosomes were isolated from serum using size exclusion chromatography following the protocol of Böing et al. [32 ]. RNA was subsequently isolated using the above mentioned Qiagen Plasma/Serum kit.