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B6.129 trpv1tm1 cre bbm j

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B6.129-Trpv1tm1(cre)Bbm/J is a transgenic mouse strain. It expresses Cre recombinase under the control of the Trpv1 gene promoter.

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Lab products found in correlation

B6 129s gt rosa 26sortm32 cag cop4 h134r eyfp hze j, by Jackson ImmunoResearch (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Ai95d, by Jackson ImmunoResearch (1 mentions)

3 protocols using b6.129 trpv1tm1 cre bbm j

1

Genetically Modified TRPV1 Sensory Neurons

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C57BL/6 mice were obtained from Jackson laboratories (Bar Harbor, ME). We generated mice expressing Ai32 (CHr2-eYFP) in TRPV1 sensory neurons (TRPV1-Ai32) by crossing homozygous Ai32 mice (B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J) with homozygous TRPV1-cre mice (B6.129-Trpv1tm1(cre)Bbm/J) both of which were purchased from Jackson laboratories. All experiments were performed using 7-to 12-week-old male mice. Mice were housed in microisolator cages and fed irradiated food and acidified water. The University of Pittsburgh Institutional Animal Care and Use Committee approved all mouse protocols.
Cohen J.A., Edwards T.N., Liu A.W., Hirai T., Jones M.R., Wu J., Li Y., Zhang S., Ho J., Davis B.M., Albers K.M, & Kaplan D.H. (2019). Cutaneous TRPV1+ Neurons Trigger Protective Innate Type-17 Anticipatory Immunity. Cell, 178(4), 919-932.e14.
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2

Transgenic Mouse Lines for TRPV1 and Ca2+ Imaging

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All animal care and experimental procedures were performed in accordance with a protocol approved by the National Institute of Health Animal Care and Use Committee. TRPV1-Cre or B6.129-TRPV1tm1(cre)Bbm/J (Stock number 017769); Rosa-LSL-tdTomato or B6.129S-Gt(ROSA)tm66.1(CAG-tdTomato)Hze/J or Ai9 (Stock number 005975); Rosa-LSL-GCaMP6f or B6J.Cg-Gt(ROSA)26Sortm95.1(CAG-GCaMP6f) Hze/MwarJ or Ai95D (Stock number 028865) strains were purchased from Jackson Laboratories (Cavanaugh et al., 2011 (link); Madisen et al., 2015 (link); Madisen et al., 2010 (link)). Calca-creERT2 was generously provided by Dr. Pao-Tien Chuang (Cardiovascular Research Institute, University of California, San Francisco, CA) (Song et al., 2012 (link)). Mice of both sexes were used in all experiments. Genotyping was performed according to information provided by Jackson Laboratories.
Ghitani N., Barik A., Szczot M., Thompson J.H., Li C., Le Pichon C.E., Krashes M.J, & Chesler A.T. (2017). Specialized mechanosensory nociceptors mediating rapid responses to hair-pull. Neuron, 95(4), 944-954.e4.
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3

Optogenetic Manipulation of TRPV1 Neurons

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All testing procedures were performed as per protocols approved by the Institutional Animal Care and Use Committee, and all efforts were made to minimize animal suffering in accordance with policies and recommendations established by the International Association for the Study of Pain. Homozygous B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J (THY1-ChR2-YFP, Stock No: 007612), B6.129-Trpv1 tm1(cre)Bbm /J (TRPV1cre, stock no: 017769) and B6.Cg-Gt(ROSA)26Sor tm32(CAG-COP4 * H134R/EYFP)Hze /J (Ai32, stock no: 012569) mice were purchased from The Jackson Laboratory. An in-house breeding program was used to generate TRPV1-ChR2-EYFP mice by crossing the TRPV1cre driver mouse line with Ai32 conditional allele mice. Only male mice, aged 6-12 weeks old were used in all experiments. Animals were housed at a maximum of five per cage (30 3 20 3 15 cm) with ad libitum access to food and water. They were kept in controlled temperature of 23 ± 1 C on a 12-hrlight/dark cycle (lights on at 7:00 a.m.). Experiments were performed between 10 a.m. and 4 p.m. Different cohorts of mice were used for each test, except when stated.
Stemkowski P., García-Caballero A., Gadotti V.M., M'Dahoma S., Huang S., Black S.A.G., Chen L., Souza I.A., Zhang Z, & Zamponi G.W. (2016). TRPV1 Nociceptor Activity Initiates USP5/T-type Channel-Mediated Plasticity. Cell reports, 17(11).
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