One percent PMSF added to RIPA lysis buffer (Beyotime, China) was used to extract total proteins. The same amounts of protein were separated by 10% SDS-PAGE, transferred to PVDF membranes (Millipore, USA), and then sealed with skim milk. The blots were incubated with the primary antibody at 4 °C for 12 h and then with the secondary antibody at room temperature for 1 h. An electrochemiluminescence kit (Millipore, USA) was used for the visualization of protein signals. β-Actin was the internal control. Primary antibodies were anti-E-cadherin, anti-N-cadherin, anti-Claudin-1, anti-Vimentin, anti-Snail, anti-OGN, anti-MMP2 and anti-EIF4A3 from Abcam (Cambridge, UK).
Anti ogn
Manufactured by Abcam
Sourced in United Kingdom
Anti-OGN is a laboratory reagent used for the detection and quantification of the OGN protein in biological samples. It is a specific antibody that binds to the OGN protein, allowing researchers to measure its levels or localize its expression within cells or tissues.
Automatically generated - may contain errors
Lab products found in correlation
Anti e cadherin, by Abcam (2 mentions)
Anti n cadherin, by Abcam (2 mentions)
Anti mmp2, by Abcam (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Electrochemiluminescence kit, by Merck Group (1 mentions)
Anti vimentin, by Abcam (1 mentions)
Anti snail, by Abcam (1 mentions)
Anti claudin 1, by Abcam (1 mentions)
Anti eif4a3, by Abcam (1 mentions)
Anti ki67, by Abcam (1 mentions)
Anti cd31, by Abcam (1 mentions)
2 protocols using anti ogn
1
Epithelial-Mesenchymal Transition Protein Profiling
2
Immunohistochemical Analysis of Tissue Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissue sections were deparaffinized with xylene and dehydrated with a graded alcohol series. Afterwards, the slices were treated with 3% hydrogen peroxide and pressure-cooked for antigen retrieval. Then, the slices were incubated with anti-OGN, anti-E-cadherin, anti-N-cadherin, anti-CD31 and anti-Ki67 primary antibodies from Abcam (Cambridge, UK) at 4 °C overnight and then incubated with the appropriate secondary antibodies for 1 h at room temperature. Finally, the sections were treated with 3'-diaminobenzidine tetrahydrochloride and counterstained with haematoxylin.
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