Naringenin extract (NR) from whole citrus sinensis oranges (purity ≥ 30%) was purchased from GE Nutrients, Inc. (Gencor, Irvine, CA). BC, lycopene, and lutein were from Cayman Chemical Co. Protease and phosphatase inhibitors were purchased from Cell Signaling Technology (Danvers, MA), TGX protein gels from BIO-RAD (Hercules, CA). Type 1 collagenase, glycerol standard solution, adenosine, estradiol, human pituitary growth hormone, dobutamine hydrochloride, human atrial natriuretic peptide, ACTH, menthol, 8-CPT-cAMP were purchased from Sigma-Aldrich. Isoproterenol, human parathyroid hormone (1–34), CDCA, were purchased from Cayman Chemicals. Glycerol reagent A was from ZenBio (Durham, NC). All other chemicals were purchased from Sigma (St. Louis, Mo) unless otherwise indicated.
Primary antibodies used were UCP1 (#MAB6158, R&D Systems), GLUT4 (Ab654 Abcam), PGC-1α (ST1202, Sigma) and β-Actin (A5316, Sigma), or monoclonals from Santa Cruz against ATGL (sc-365278), adiponectin (sc-136131), PPARα (398394), PPARγ (sc-7273), NAMPT (sc-393444), RXRγ (sc-514134), RXRα (sc-515929). HRP-linked anti-rabbit (12-348, Sigma), anti-mouse (AP130P, Sigma) and anti-IgG kappa light chain (sc-516102, Santa Cruz) were used to detect specific antibody-antigen complexes. Western Lightning Plus-ECL was from PerkinElmer (Waltham, MA).
Primary antibodies used were UCP1 (#MAB6158, R&D Systems), GLUT4 (Ab654 Abcam), PGC-1α (ST1202, Sigma) and β-Actin (A5316, Sigma), or monoclonals from Santa Cruz against ATGL (sc-365278), adiponectin (sc-136131), PPARα (398394), PPARγ (sc-7273), NAMPT (sc-393444), RXRγ (sc-514134), RXRα (sc-515929). HRP-linked anti-rabbit (12-348, Sigma), anti-mouse (AP130P, Sigma) and anti-IgG kappa light chain (sc-516102, Santa Cruz) were used to detect specific antibody-antigen complexes. Western Lightning Plus-ECL was from PerkinElmer (Waltham, MA).