Paramagnetic particle chemiluminescent immunoassay

Testosterone was measured with a chemiluminescent immunoassay for quantitative determination of total testosterone level in human serum and plasma using the Access Immunoassay System (Beckman Coulter). The detection rate range was between 0.35–55.5 nmol/L). The intra- and inter-assay CV for testosterone were less than 5 percent in both cases. Sexual hormone binding globulin (SHBG) and prolactin were measured with a paramagnetic particle chemiluminescent immunoassay (Beckman Coulter, Inc). The intra-and inter-assay variation for SHBG was 4.0 and 5.5 percent respectively, and for prolactin 3.5 and 5.0 percent. Total cholesterol, High-density lipoprotein (HDL)-cholesterol and HbA1c were measured by the routine chemistry accredited laboratory at the Karolinska University Hospital. LDL-cholesterol was calculated according to the Friedewald formula [10 (link)]. All samples were analyzed according to the manufacturer’s advice at the Department of clinical chemistry, Karolinska University Hospital.