Western blot analysis was done, as explained below [16 (link),22 (link)]. The primary antibodies were treated during 24 hours for TNF-α (1:1,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), IL-1β (1:1,000; Santa Cruz Biotechnology), NF-κB (1:1,000; Abcam, Cambridge, UK), IκBα (1:1,000; Santa Cruz Biotechnology), phosphorylated IκBα (p-IκBα) (1:1,000; Santa Cruz Biotechnology), extracellular signal-regulated kinase (ERK) (1:1,000; Cell Signaling Technology), phosphorylated ERK (1:1,000; Cell Signaling Technology), c-Jun N-terminal kinases (JNK) (1:1,000; Cell Signaling Technology), phosphorylated JNK (1:1,000; Cell Signaling Technology), p38 (1:1,000; Cell Signaling Technology), and phosphorylated p38 (1:1,000; Cell Signaling Technology). The secondary antibodies were applied to the membrane, and then the bands were calculated by enhanced chemiluminescent kit.
Phosphorylated iκbα p iκbα
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom, United States
Phosphorylated IκBα (p-IκBα) is a laboratory reagent that represents the phosphorylated form of the inhibitor of NF-κB (IκBα) protein. IκBα is a key regulator of the NF-κB signaling pathway, and its phosphorylation is a crucial step in the activation of NF-κB. p-IκBα is a valuable tool for studying the dynamics and regulation of the NF-κB signaling cascade.
Automatically generated - may contain errors
Lab products found in correlation
Phosphorylated p38, by Cell Signaling Technology (1 mentions)
Extracellular signal regulated kinase erk, by Cell Signaling Technology (1 mentions)
Phosphorylated jnk, by Cell Signaling Technology (1 mentions)
Il 1β, by Santa Cruz Biotechnology (1 mentions)
Tnf α, by Santa Cruz Biotechnology (1 mentions)
C jun n terminal kinases jnk, by Cell Signaling Technology (1 mentions)
Phosphorylated erk, by Cell Signaling Technology (1 mentions)
Nf κb, by Abcam (1 mentions)
Cerulein, by Merck Group (1 mentions)
Horseradish peroxidase conjugated, by Santa Cruz Biotechnology (1 mentions)
Nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit antibody, by Santa Cruz Biotechnology (1 mentions)
Tnf α, by R&D Systems (1 mentions)
2 protocols using phosphorylated iκbα p iκbα
1
Western Blot Analysis of Inflammatory Markers
2
Cerulein-Induced Pancreatitis Model
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Cerulein was purchased from Sigma (St. Louis, MO, USA). Antibodies against the p65 subunit of nuclear factor-κB (NF-κB p65), I-κBα, phosphorylated-I-κBα (p-I-κBα), thioredoxin-interacting protein (TXNIP), NLRP3, p20 subunit of caspase-1 (casp-1-p20), β-actin, and horseradish peroxidase conjugated goat anti-rabbit antibody were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Enzyme-linked immunosorbent assay (ELISA) kits for IL-1β and tumor necrosis factor-α (TNF-α) were obtained from R&D system (Minneapolis, MN, USA).
HRS was prepared as described previously [15 (link)]. In brief, H2 gas (0.4 MPa) was dissolved in normal saline (NS) for 6 hours to achieve a concentration of 0.6 mmol/L, and the concentration of H2 in NS was detected using a dissolved hydrogen analyzer (DH-35A; DKKTOA, Tokyo, Japan). The HRS was freshly prepared every week and then stored under atmospheric pressure at 4°C in an aluminum bag with no dead volume and sterilized by gamma radiation before use.
HRS was prepared as described previously [15 (link)]. In brief, H2 gas (0.4 MPa) was dissolved in normal saline (NS) for 6 hours to achieve a concentration of 0.6 mmol/L, and the concentration of H2 in NS was detected using a dissolved hydrogen analyzer (DH-35A; DKKTOA, Tokyo, Japan). The HRS was freshly prepared every week and then stored under atmospheric pressure at 4°C in an aluminum bag with no dead volume and sterilized by gamma radiation before use.
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