Log In Sign up
The largest database of trusted experimental protocols

Pbmn gfp

Manufactured by Addgene
Visit Supplier
Sourced in United States

The PBMN-GFP is a plasmid vector that expresses the green fluorescent protein (GFP) under the control of a constitutive promoter. This vector is commonly used for the expression and detection of GFP in various cell lines and organisms.

Automatically generated - may contain errors

Lab products found in correlation

Plvx ires mcherry, by Takara Bio (2 mentions) Pcdna3 flag fkhrl1, by Addgene (2 mentions) Plvx ires mcherry, by Addgene (2 mentions)

Spelling variants of this product

PBMN-I-GFP (9 citations) PBMN-IRES-GFP (2 citations) PBMN-I-GFP retroviral expressing vector (2 citations)

2 protocols using pbmn gfp

1

Establishing HNSCC Cell Lines and Genetic Manipulations

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The STR-verified HNSCC cell lines UM-SCC-1, UMSCC-17B, Detroit 562, PCI-13, MDA1586, PCI-15B, TR146, and Ca9-22 were described previously [46 (link)], and STAR cells was established in our laboratory. Cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS. Stable cell lines expressing control vector pBabe; wild-type p53; mutant p53s G245D, C238F, R175H, and E336X; and short hairpin RNAs (shRNAs) for p53 (shp53) and AMPKα1 (shAMPK α1) were established as described previously [9 (link), 16 (link)]. pBMN-GFP (Addgene, Cambridge, MA, USA), pLVX-IRES-mCherry (Takara Bio USA, Mountain View, CA, USA), and the PCR product from pcDNA3-FLAG-FKHRL1 (Addgene) were used to generate retroviral pBMN-GFP-FOXO3a and lentiviral pLVX-IRES-mCherry-FOXO3a expression. Lentiviral pGIPz shRNA constructs shFOXO3A-A (V3LHS_375381), shFOXO3A-B (V3LHS_375386), shFOXM1-A (V2LHS_283849), shFOXM1-C (V3LHS_396939), shp53-A (V3LHS_333920), and shp53-C (V3LHS_333919) were from Dharmacon (Lafayette, CO, USA). AMPKα1 and FOXO3a CRISPR/Cas9 knockout (KO) plasmids sc-400104 and sc-400308 were from Santa Cruz Technologies (Dallas, TX, USA).
Tanaka N., Zhao M., Tang L., Patel A.A., Xi Q., Van H.T., Takahashi H., Osman A.A., Zhang J., Wang J., Myers J.N, & Zhou G. (2017). Gain-of-function mutant p53 promotes the oncogenic potential of head and neck squamous cell carcinoma cells by targeting the transcription factors FOXO3a and FOXM1. Oncogene, 37(10), 1279-1292.
+ Open protocol
+ Expand
2

Cell Line Generation and Manipulation Protocols

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The STR-verified HNSCC cell lines UM-SCC-1, UMSCC-17B, Detroit 562, PCI-13, MDA1586, PCI-15B, TR146, and Ca9-22 were described previously,46 (link) and STAR cells was established in our laboratory. Cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS. Stable cell lines expressing control vector pBabe; wild-type p53; mutant p53s G245D, C238F, R175H, and E336X; and short hairpin RNAs (shRNAs) for p53 (shp53) and AMPKα1 (shAMPK α1) were established as described previously.9 (link),16 (link) pBMN-GFP (Orbigen), pLVX-IRES-mCherry (Clontech), and the PCR product from pcDNA3-FLAG-FKHRL1 (Addgene) were used to generate retroviral pBMN-GFP-FOXO3a and lentiviral pLVX-IRES-mCherry-FOXO3a expression. Lentiviral pGIPz shRNA constructs shFOXO3A-A (V3LHS_375381), shFOXO3A-B (V3LHS_375386), shFOXM1-A (V2LHS_283849), shFOXM1-C (V3LHS_396939), shp53-A (V3LHS_333920), and shp53-C (V3LHS_333919) were from Open Biosystems. AMPKα1 and FOXO3a CRISPR/Cas9 knockout (KO) plasmids sc-400104 and sc-400308 were from Santa Cruz Technologies.
Tanaka N., Zhao M., Tang L., Patel A.A., Xi Q., Van H.T., Takahashi H., Osman A.A., Zhang J., Wang J., Myers J.N, & Zhou G. (2017). Gain-of-function mutant p53 promotes the oncogenic potential of head and neck squamous cell carcinoma cells by targeting the transcription factors FOXO3a and FOXM1. Oncogene, 37(10), 1279-1292.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!