Af3169

We performed protein extraction and western blot analyses as described previously [29 (link)]. The utilized antibodies included goat antibody to HO-1 (AF3169, 1:500; R&D System), GAPDH (AF5718, 1:500; R&D System), goat anti-mouse IgG (sc-2039, 1:5,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA). The proteins were visualized using an enhanced chemiluminescence detection kit (Bio-Rad, USA) following the manufacturer’s protocol. Images were analyzed using the Kodak Image Station 2000R (Eastman Kodak Company, Rochester, NY, USA). Protein bands intensity were normalized to GAPDH, and the data expressed in terms of percent relative to controls.

We performed protein extraction and western blot analyses as described previously^{20 (link)}. The utilized antibodies included mouse antibody to NQO1 (sc-376023, 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), Nrf2 (MAB3925, 1:500; R&D System), NFκB (p65) (sc-8008, 1:200; Santa Cruz Biotechnology), goat antibody to HO-1 (AF3169, 1:500; R&D System), β-actin (MAB8929, 1:500; R&D System), goat anti-mouse IgG (sc-2039, 1:5,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and donkey anti-goat IgG (sc-2042, 1:5,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA). The proteins were visualized using an enhanced chemiluminescence detection kit (Bio-Rad, USA) following the manufacturer’s protocol. Images were analyzed using the Kodak Image Station 2000R (Eastman Kodak Company, Rochester, NY, USA). Protein bands intensity were normalized to β-actin, and the data expressed in terms of percent relative to controls.